##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805400_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15701452
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36364452153852	35.0	28.0	37.0	28.0	37.0
2	34.826976065653035	35.0	35.0	37.0	32.0	37.0
3	37.448420311701106	39.0	37.0	39.0	35.0	39.0
4	37.77927977616338	39.0	37.0	39.0	35.0	39.0
5	37.796003070289295	39.0	37.0	39.0	35.0	39.0
6	37.831739765214074	39.0	38.0	39.0	35.0	39.0
7	37.82759874691844	39.0	38.0	39.0	35.0	39.0
8	38.84376833429163	40.0	39.0	40.0	36.0	40.0
9	38.84581585193522	40.0	39.0	40.0	36.0	40.0
10	38.7449974053355	40.0	39.0	40.0	36.0	40.0
11	38.76326170343991	40.0	39.0	40.0	36.0	40.0
12	38.702331032824226	40.0	39.0	40.0	36.0	40.0
13	38.80827881395937	40.0	39.0	40.0	36.0	40.0
14	38.74584688091267	40.0	39.0	40.0	36.0	40.0
15	38.78078021064549	40.0	39.0	40.0	36.0	40.0
16	38.771214662185386	40.0	39.0	40.0	36.0	40.0
17	38.58697526827455	40.0	39.0	40.0	36.0	40.0
18	38.59119156623222	40.0	39.0	40.0	36.0	40.0
19	38.598442742747615	40.0	39.0	40.0	36.0	40.0
20	38.44195746992062	40.0	39.0	40.0	35.0	40.0
21	38.421059593724195	40.0	39.0	40.0	35.0	40.0
22	38.34976134691237	40.0	39.0	40.0	35.0	40.0
23	38.38016312121962	40.0	38.0	40.0	35.0	40.0
24	37.85063464194267	40.0	38.0	40.0	34.0	40.0
25	38.09040826287913	40.0	38.0	40.0	34.0	40.0
26	38.1095147123973	40.0	38.0	40.0	34.0	40.0
27	38.07622180420002	40.0	38.0	40.0	34.0	40.0
28	38.07211046468824	40.0	38.0	40.0	34.0	40.0
29	38.03278028044795	40.0	38.0	40.0	34.0	40.0
30	37.89880585566227	40.0	38.0	40.0	34.0	40.0
31	37.75755923719666	40.0	38.0	40.0	33.0	40.0
32	37.76285333356431	40.0	38.0	40.0	33.0	40.0
33	37.76720420506333	40.0	38.0	40.0	33.0	40.0
34	37.74763486841854	40.0	38.0	40.0	33.0	40.0
35	37.67159928903391	40.0	38.0	40.0	33.0	40.0
36	37.623951402711036	40.0	38.0	40.0	33.0	40.0
37	37.513492446431066	40.0	37.0	40.0	33.0	40.0
38	37.38745671419433	40.0	37.0	40.0	33.0	40.0
39	30.609193340845167	38.0	29.0	40.0	3.0	40.0
40	32.651452999378655	38.0	29.0	40.0	15.0	40.0
41	34.93056845952846	38.0	30.0	40.0	25.0	40.0
42	36.15072835302111	38.0	35.0	40.0	30.0	40.0
43	36.57474538023617	39.0	36.0	40.0	31.0	40.0
44	36.67889116242243	39.0	36.0	40.0	31.0	40.0
45	36.6892347281003	39.0	36.0	40.0	31.0	40.0
46	36.47245063704936	39.0	36.0	40.0	30.0	40.0
47	36.376742736913755	39.0	35.0	40.0	30.0	40.0
48	36.208503774045866	39.0	35.0	40.0	30.0	40.0
49	36.52766693169524	39.0	36.0	40.0	30.0	40.0
50	36.60684871692121	39.0	36.0	40.0	31.0	40.0
51	36.61410537063706	39.0	35.0	40.0	31.0	40.0
52	36.521618255432685	39.0	35.0	40.0	31.0	40.0
53	36.326955430618774	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	31.0
14	89.0
15	255.0
16	641.0
17	1260.0
18	2399.0
19	4195.0
20	7379.0
21	12272.0
22	18586.0
23	27532.0
24	38564.0
25	52910.0
26	70224.0
27	91755.0
28	118692.0
29	151606.0
30	192574.0
31	241223.0
32	306210.0
33	398979.0
34	541383.0
35	777237.0
36	1248867.0
37	2498248.0
38	3804493.0
39	5093840.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.388290458742286	28.828072715822717	24.641045936388558	33.14259088904644
2	24.331565004306608	24.625849889551617	30.305744971866293	20.736840134275482
3	16.492735831055626	25.83182752779807	31.540942837643293	26.134493803503013
4	17.88019350057562	27.860366034937407	31.207661558943723	23.05177890554326
5	23.682854299080113	27.513984057015872	29.0916534343448	19.711508209559216
6	22.186292070312987	26.823442825542504	29.373512717167817	21.616752386976696
7	23.45216862746197	27.58238537429532	28.383445046993106	20.582000951249604
8	21.488554052198484	25.826044623134216	29.395746329702497	23.2896549949648
9	21.240596092641624	25.24642943850034	30.909962976672478	22.603011492185562
10	22.397992236641553	26.350957860457747	28.286250214311394	22.96479968858931
11	22.051890487580383	27.10548680465985	27.97662916779926	22.86599353996051
12	22.190909477671237	26.71419178302746	28.131850481089266	22.963048258212044
13	22.39535553781905	26.956997352856284	27.009788648845976	23.637858460478686
14	23.041257585604185	27.279317861813034	26.842421961994344	22.837002590588437
15	22.515732939858047	27.190689115885586	27.109027878440795	23.184550065815568
16	23.00854086615684	26.95541151226014	26.560467146605294	23.47558047497773
17	24.002079552897403	26.366160276132423	26.74348843661083	22.888271734359343
18	22.97995752239984	26.664311045882886	26.619353420307878	23.73637801140939
19	23.200720544826044	26.972817545791305	26.515420357301988	23.31104155208066
20	23.859857037425584	26.481620935439604	26.587356379524646	23.07116564761017
21	23.34175208764132	26.922369982088277	26.751169254919866	22.984708675350536
22	23.531409706567267	26.345245013008988	26.879208368754686	23.24413691166906
23	23.054657620199713	26.62172899678323	27.299628085351596	23.023985297665465
24	22.824666151894743	26.786987598344407	27.192357751372292	23.195988498388555
25	22.88027884300127	26.49387457924274	26.9200963070167	23.705750270739294
26	23.050670727777277	26.637370862261655	27.26003938998763	23.05191901997344
27	23.25381117618931	26.167904726263536	27.56569901942827	23.012585078118892
28	23.657410792326722	26.308254803441113	26.76677927620961	23.267555128022554
29	23.672836117322145	26.329666835907915	26.869833439608005	23.127663607161935
30	23.377825184575286	26.29111626109483	27.100843921950656	23.230214632379223
31	23.5295691124617	26.350371927386078	26.82467201122546	23.29538694892676
32	23.681841653880163	26.49842829822363	26.749105751493556	23.07062429640265
33	23.24407959212944	26.61471053759869	26.79133114568003	23.34987872459184
34	23.35776334570841	26.659362458962395	26.543933643843893	23.438940551485302
35	23.47940177761904	26.631307728737447	26.712898908967148	23.176391584676374
36	23.24615583323122	26.612959107221425	26.755984096247914	23.384900963299447
37	23.363208701972276	26.688640006032564	26.470666534534516	23.477484757460648
38	23.56802415470875	26.725114339743865	26.655152657219215	23.05170884832817
39	20.16066412201878	39.257694129179896	21.878715420713956	18.70292632808736
40	23.402905667577752	26.640810034638836	26.550213317851114	23.406070979932302
41	23.481134101483097	26.63353045310714	26.7496152585124	23.135720186897366
42	23.313085948993763	26.4963074752577	26.842332798266042	23.34827377748249
43	23.36829103448522	26.53590253945941	26.589088703388708	23.506717722666668
44	23.51859560504341	26.54844278096064	26.709663539397503	23.22329807459845
45	23.392358872287737	26.378356600395936	26.84772083499029	23.381563692326036
46	23.594989813680925	26.4032396494286	26.571854628476398	23.429915908414074
47	23.58205470424009	26.52248976718841	26.64918505626104	23.24627047231046
48	23.352617324818112	26.542965580508092	26.67769197396521	23.426725120708582
49	23.482649884864152	26.490919438533457	26.51133156347579	23.51509911312661
50	23.62309549460776	26.48380544678288	26.677806613044446	23.215292445564906
51	23.434259455749697	26.413939296824267	26.73083355603036	23.420967691395674
52	23.532772637842665	26.484773510118682	26.37920365581476	23.60325019622389
53	23.7316459649719	26.458782283320037	26.531552623286046	23.27801912842201
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3718.0
1	2521.0
2	1324.0
3	1153.5
4	983.0
5	1021.5
6	1060.0
7	1298.5
8	1951.5
9	2366.0
10	3444.0
11	4522.0
12	6438.0
13	8354.0
14	10971.0
15	13588.0
16	19301.0
17	25014.0
18	34457.5
19	43901.0
20	59216.0
21	74531.0
22	107853.0
23	141175.0
24	153477.0
25	195938.5
26	226098.0
27	260204.0
28	294310.0
29	332232.5
30	370155.0
31	407434.5
32	444714.0
33	492510.5
34	540307.0
35	598152.0
36	655997.0
37	709996.0
38	763995.0
39	812974.0
40	861953.0
41	905512.5
42	987196.5
43	1025321.0
44	1047073.0
45	1068825.0
46	1080185.0
47	1091545.0
48	1092952.0
49	1094359.0
50	1083066.5
51	1071774.0
52	1044493.0
53	1017212.0
54	967035.5
55	916859.0
56	851672.5
57	786486.0
58	713768.5
59	563626.0
60	486201.0
61	417330.0
62	348459.0
63	291340.5
64	234222.0
65	189644.5
66	145067.0
67	115518.5
68	85970.0
69	66036.0
70	46102.0
71	34990.0
72	23878.0
73	17905.0
74	11932.0
75	8995.5
76	4512.0
77	2965.0
78	2182.5
79	1400.0
80	1050.5
81	701.0
82	516.0
83	331.0
84	232.0
85	133.0
86	101.0
87	69.0
88	48.0
89	27.0
90	27.5
91	28.0
92	20.0
93	7.5
94	3.0
95	4.5
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.5701452E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.00995768156903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.81123665014907	43.97806629862562
2	13.940507368407676	16.17376485001861
3	4.3570801874932235	7.5826211187505415
4	1.9389292606631243	4.4990881743449505
5	1.0189603807556227	2.9554924283414565
6	0.6569748796069304	2.2866650978311163
7	0.43245956498424687	1.7560872744608138
8	0.300163025724895	1.39299555358983
9	0.22909380354481268	1.1960749663869845
>10	1.2050164541544297	12.331531174346713
>50	0.08013533804708403	3.2436990372666887
>100	0.029309217576522127	2.4033602968841667
>500	6.737568868531179E-5	0.026791284069809396
>1k	4.433542197665914E-5	0.044132680782795475
>5k	1.1078890870899665E-5	0.036099672090856745
>10k+	1.1078890870899662E-5	0.09353009220905076
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5028607545340394E-4	0.0	0.0	6.368837735516435E-6	1.273767547103287E-5
2	4.012367773375354E-4	0.0	0.0	6.368837735516435E-6	1.9106513206549306E-5
3	4.012367773375354E-4	0.0	0.0	6.368837735516435E-6	2.547535094206574E-5
4	4.0760561507305186E-4	0.0	0.0	6.368837735516435E-6	3.821302641309861E-5
5	5.986707471385449E-4	0.0	0.0	6.368837735516435E-6	3.821302641309861E-5
6	5.986707471385449E-4	0.0	0.0	6.368837735516435E-6	5.7319539619647914E-5
7	6.050395848740613E-4	0.0	0.0	6.368837735516435E-6	5.7319539619647914E-5
8	6.114084226095778E-4	0.0	0.0	1.9106513206549306E-5	7.642605282619722E-5
9	6.177772603450942E-4	0.0	0.0	1.9106513206549306E-5	7.642605282619722E-5
10	6.30514935816127E-4	0.0	0.0	1.9106513206549306E-5	7.642605282619722E-5
11	6.623591244937092E-4	0.0	0.0	1.9106513206549306E-5	7.642605282619722E-5
12	8.024735546750708E-4	0.0	0.0	1.9106513206549306E-5	8.916372829723009E-5
13	0.0015348898942594608	0.0	0.0	1.9106513206549306E-5	8.916372829723009E-5
14	0.0017577992150025362	0.0	0.0	1.9106513206549306E-5	8.916372829723009E-5
15	0.0021144541281914566	0.0	0.0	2.547535094206574E-5	9.553256603274653E-5
16	0.002209986694224203	0.0	0.0	2.547535094206574E-5	1.0190140376826296E-4
17	0.002254568558372818	0.0	0.0	2.547535094206574E-5	1.2100791697481226E-4
18	0.0024520025281738276	0.0	0.0	2.547535094206574E-5	1.2100791697481226E-4
19	0.002522059743264508	6.368837735516435E-6	0.0	2.547535094206574E-5	1.2100791697481226E-4
20	0.0025666416074131234	1.9106513206549306E-5	0.0	2.547535094206574E-5	1.2100791697481226E-4
21	0.00266217417344587	1.9106513206549306E-5	0.0	2.547535094206574E-5	1.2100791697481226E-4
22	0.0027194937130655176	1.9106513206549306E-5	0.0	3.184418867758217E-5	1.4011443018136158E-4
23	0.002821395116833781	1.9106513206549306E-5	0.0	3.184418867758217E-5	1.5285210565239445E-4
24	0.002910558845131011	1.9106513206549306E-5	0.0	3.821302641309861E-5	1.5922094338791088E-4
25	0.0030251979243703067	1.9106513206549306E-5	0.0	4.458186414861504E-5	1.655897811234273E-4
26	0.003209894218700283	1.9106513206549306E-5	0.0	5.095070188413148E-5	1.655897811234273E-4
27	0.0034200658639723255	1.9106513206549306E-5	0.0	5.095070188413148E-5	1.655897811234273E-4
28	0.0034455412149143913	1.9106513206549306E-5	0.0	1.273767547103287E-4	1.655897811234273E-4
29	0.0037894584526322788	1.9106513206549306E-5	0.0	1.273767547103287E-4	1.655897811234273E-4
30	0.0038849910186650252	1.9106513206549306E-5	0.0	1.974339698010095E-4	1.7832745659446017E-4
31	0.004044211962052936	2.547535094206574E-5	0.0	1.974339698010095E-4	1.846962943299766E-4
32	0.004114269177143617	2.547535094206574E-5	0.0	1.974339698010095E-4	1.9106513206549306E-4
33	0.004235277094118429	2.547535094206574E-5	0.0	1.974339698010095E-4	1.9106513206549306E-4
34	0.004298965471473594	2.547535094206574E-5	0.0	1.974339698010095E-4	1.9106513206549306E-4
35	0.004432711063919439	2.547535094206574E-5	0.0	1.974339698010095E-4	2.356469962141081E-4
36	0.004623776195984932	2.547535094206574E-5	0.0	1.974339698010095E-4	2.547535094206574E-4
37	0.004802103652579392	2.547535094206574E-5	0.0	1.974339698010095E-4	2.547535094206574E-4
38	0.004904005056347655	2.547535094206574E-5	0.0	1.974339698010095E-4	2.6112234715617384E-4
39	0.005044119486529016	2.547535094206574E-5	0.0	1.974339698010095E-4	2.6749118489169025E-4
40	0.0051969715921814106	2.547535094206574E-5	0.0	1.974339698010095E-4	2.8659769809823957E-4
41	0.005311610671420707	2.547535094206574E-5	0.0	1.974339698010095E-4	2.9296653583375603E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGTA	4385	0.0	34.620296	10
TATGCCG	4450	0.0	33.95618	15
CTCGTAT	4580	0.0	32.88974	11
TCGTATG	4775	0.0	31.645025	12
CGTATGC	5145	0.0	29.689018	13
ATGCCGT	5040	0.0	29.375	16
CCGTCTT	5310	0.0	27.881355	19
TGCCGTC	5355	0.0	27.64706	17
GCCGTCT	5330	0.0	27.644464	18
GAGCTCG	5640	0.0	27.166666	8
ATCGGAA	5730	0.0	26.698954	1
AAGCAGT	15280	0.0	24.868782	1
AGCTCGT	6360	0.0	24.128145	9
GTATGCC	6740	0.0	22.977001	14
CAACGCA	17415	0.0	22.427217	13
TCAACGC	17390	0.0	22.418919	12
AACGCAG	17955	0.0	21.831245	14
ACGCAGA	18185	0.0	21.490513	15
ATCAACG	18460	0.0	21.23402	11
CGTCTTC	7140	0.0	20.866947	20
>>END_MODULE