FastQCFastQC Report
Thu 26 May 2016
SRR805399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15203092
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT218710.14385889396709564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT173000.028.7161871
CAACGCA240500.021.74116313
TCAACGC240250.021.73444412
AACGCAG246850.021.2199714
AGTGGTA248250.021.0908365
GTATCAA249000.021.07459
GGTATCA248850.021.0494278
ACGCAGA250000.021.00915
GTGGTAT248750.020.9728646
ATCAACG250500.020.92015811
TGGTATC254250.020.574637
AGCAGTG256650.020.4921112
CGCAGAG261050.020.12871216
GCAGTGG262600.019.9741043
TATCAAC264650.019.84602210
CAGTGGT274000.019.185954
AGAGTAC262950.019.03593819
CAGAGTA278250.018.1412418
GCAGAGT286250.017.86410517
ACATGGG202400.017.25345824
Produced by FastQC (version 0.11.5)