##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805399_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15203092
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.320791389014815	35.0	35.0	37.0	35.0	37.0
2	35.77831371407869	37.0	35.0	37.0	35.0	37.0
3	37.73087047029644	39.0	38.0	39.0	35.0	39.0
4	37.79117228258568	39.0	38.0	39.0	35.0	39.0
5	37.741992549936555	39.0	38.0	39.0	35.0	39.0
6	37.64939276826056	39.0	38.0	39.0	35.0	39.0
7	37.66054714396256	39.0	38.0	39.0	35.0	39.0
8	38.6679889196224	40.0	39.0	40.0	36.0	40.0
9	38.64387237806625	40.0	39.0	40.0	36.0	40.0
10	38.64679599386756	40.0	39.0	40.0	36.0	40.0
11	38.62955667176124	40.0	39.0	40.0	36.0	40.0
12	38.58259609295267	40.0	39.0	40.0	36.0	40.0
13	38.639921537013656	40.0	39.0	40.0	36.0	40.0
14	38.63497076778855	40.0	39.0	40.0	36.0	40.0
15	38.64285817648147	40.0	39.0	40.0	36.0	40.0
16	38.614627340280514	40.0	39.0	40.0	36.0	40.0
17	38.55249958363733	40.0	39.0	40.0	36.0	40.0
18	38.46449307811858	40.0	39.0	40.0	35.0	40.0
19	38.46116119010527	40.0	39.0	40.0	35.0	40.0
20	38.392720638670085	40.0	39.0	40.0	35.0	40.0
21	38.36656115742771	40.0	39.0	40.0	35.0	40.0
22	38.34664165684191	40.0	39.0	40.0	35.0	40.0
23	37.41976105913192	40.0	38.0	40.0	34.0	40.0
24	37.741064975466834	40.0	38.0	40.0	33.0	40.0
25	37.99483434027762	40.0	38.0	40.0	33.0	40.0
26	37.97710090815737	40.0	38.0	40.0	34.0	40.0
27	38.0326124448895	40.0	38.0	40.0	34.0	40.0
28	38.071963650552135	40.0	38.0	40.0	34.0	40.0
29	37.86027434419262	40.0	38.0	40.0	34.0	40.0
30	37.90108808129294	40.0	38.0	40.0	34.0	40.0
31	37.75488920280164	40.0	38.0	40.0	33.0	40.0
32	37.905987676717345	40.0	38.0	40.0	34.0	40.0
33	37.74161585024941	40.0	38.0	40.0	33.0	40.0
34	37.9079579995964	40.0	38.0	40.0	34.0	40.0
35	37.967167007869186	40.0	38.0	40.0	34.0	40.0
36	37.99690556368402	40.0	38.0	40.0	34.0	40.0
37	37.795493706148726	40.0	38.0	40.0	33.0	40.0
38	37.81992432855106	40.0	38.0	40.0	33.0	40.0
39	37.87815623295577	40.0	38.0	40.0	33.0	40.0
40	37.8627478541865	40.0	38.0	40.0	33.0	40.0
41	37.77934435968683	40.0	38.0	40.0	33.0	40.0
42	37.731316169105604	40.0	38.0	40.0	33.0	40.0
43	37.114152765766335	40.0	37.0	40.0	31.0	40.0
44	37.13594491173243	40.0	37.0	40.0	31.0	40.0
45	37.314062889312254	40.0	37.0	40.0	32.0	40.0
46	37.329592098765175	40.0	37.0	40.0	32.0	40.0
47	37.339942690605305	40.0	37.0	40.0	33.0	40.0
48	37.34774702409221	40.0	37.0	40.0	33.0	40.0
49	37.17838936974137	40.0	37.0	40.0	32.0	40.0
50	37.09560989304018	40.0	37.0	40.0	32.0	40.0
51	36.93621869814377	40.0	36.0	40.0	31.0	40.0
52	36.911946859231	40.0	36.0	40.0	31.0	40.0
53	36.53590684052954	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	10.0
11	37.0
12	83.0
13	250.0
14	420.0
15	712.0
16	1123.0
17	1941.0
18	3353.0
19	5550.0
20	8876.0
21	13751.0
22	20319.0
23	28278.0
24	39022.0
25	51586.0
26	66874.0
27	85016.0
28	107520.0
29	133778.0
30	166334.0
31	206123.0
32	255930.0
33	320801.0
34	414607.0
35	563681.0
36	829993.0
37	1375186.0
38	2573457.0
39	7928478.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.72052474588722	28.04849829232106	27.293493981355898	30.93748298043582
2	24.968203836430117	25.01556920131773	29.91889413022035	20.0973328320318
3	16.81394153241985	25.595359154571977	32.04971725488473	25.54098205812344
4	17.69601210069636	28.273268358831217	31.53226330538551	22.498456235086913
5	23.565074788733764	27.83706103995161	29.129317904542052	19.468546266772574
6	22.373126466642443	26.737975406581764	29.591033192458482	21.29786493431731
7	23.268944238448334	27.42944658889126	28.90760642637695	20.394002746283455
8	21.635263405628276	25.88279410530437	29.339176530668894	23.142765958398464
9	21.373514019385002	25.193815836936327	30.975146371540735	22.45752377213793
10	22.112528162034405	26.590360697679127	28.516600438910718	22.780510701375746
11	21.9693664946578	27.39572976339287	28.099849688471267	22.535054053478067
12	22.21953928845527	26.808033523706886	28.14198585393024	22.830441333907604
13	22.31966365789275	27.113076734653713	27.134204015867297	23.43305559158624
14	22.69267330619324	27.643751678934787	27.004315964147292	22.659259050724682
15	22.390866279043763	27.398295031037108	27.01447837058409	23.196360319335042
16	22.891330263606903	26.84920935820161	26.766140729793648	23.49331964839784
17	23.814195165036164	26.479449048917154	26.706915935258436	22.999439850788246
18	22.882489956648293	26.816413398011406	26.65823504850198	23.642861596838326
19	23.24375857226938	27.209096675860412	26.630352562491893	22.916792189378317
20	23.667915710830403	26.709093123951366	26.765923668685293	22.85706749653294
21	23.211824278903265	27.14979295001306	26.82912791687375	22.80925485420992
22	23.481716745514664	26.434313493597223	26.994456127740328	23.089513633147785
23	22.47032379992175	28.49352618533125	26.889747164589938	22.14640285015706
24	22.763882504953596	27.104335091835267	26.901475042050656	23.23030736116048
25	22.836400647973452	26.62249231932557	26.826667890979017	23.71443914172196
26	23.189342010164772	26.73341054569689	27.40702351863687	22.67022392550147
27	23.18880264619855	26.22252762793253	27.67212090803634	22.916548817832584
28	23.616518271414787	26.32457923690786	26.977084661462285	23.081817830215066
29	23.73909202154404	26.327183970208168	27.0080520462548	22.925671961992993
30	23.52495137173412	26.29867003370104	27.079300710671223	23.09707788389362
31	23.406363652867455	26.424519433283706	26.925332031142084	23.243784882706755
32	23.431720336889363	26.59293254293271	26.972026479876593	23.00332064030133
33	23.25828193370138	26.48324432950876	27.016083307264076	23.242390429525784
34	23.261248435515615	26.53126087772145	26.844999688221314	23.362490998541613
35	23.360682155971958	26.51652045518109	26.975177154752465	23.147620234094486
36	23.238812210042536	26.446968813975474	26.992443379281006	23.321775596700984
37	23.503350502647752	26.49779400137814	26.77044248630476	23.22841300966935
38	23.50283087150956	26.61136958192452	26.869764387402245	23.016035159163675
39	23.273055244288464	26.554611390893378	26.94326917182373	23.229064192994425
40	23.345343170981273	26.58397383900591	26.752663208247373	23.318019781765447
41	23.38279607858717	26.61154059976747	26.950964974756452	23.05469834688891
42	23.256894058129753	26.475305155030306	27.035572763750952	23.23222802308899
43	23.324097492799492	26.40059009048949	26.865192948908025	23.410119467802996
44	23.59518050670219	26.466912125507104	26.858944220031027	23.07896314775968
45	23.334259899236287	26.379087885543285	27.007519259897922	23.27913295532251
46	23.29140019674945	26.439075682762425	26.83819844015941	23.43132568032871
47	23.422366976401907	26.50284560535449	26.8545503769891	23.220237041254503
48	23.25561800191698	26.374483559002336	26.981333797098646	23.388564641982036
49	23.38792003626631	26.519822415071882	26.689005105014164	23.40325244364765
50	23.544079059707066	26.568378327250798	26.770389865430005	23.11715274761213
51	23.385663916261244	26.455026385422126	26.880150432556743	23.279159265759887
52	23.465437162387754	26.4711678387528	26.61369805563237	23.449696943227075
53	23.56841621428062	26.530458409381456	26.642823709808503	23.258301666529412
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7854.0
1	5270.5
2	2687.0
3	2164.5
4	1642.0
5	1597.0
6	1552.0
7	1821.0
8	2459.5
9	2829.0
10	4094.0
11	5359.0
12	7744.0
13	10129.0
14	13115.0
15	16101.0
16	22557.5
17	29014.0
18	38900.0
19	48786.0
20	65143.5
21	81501.0
22	121108.0
23	160715.0
24	170305.0
25	209066.5
26	238238.0
27	272559.5
28	306881.0
29	341496.5
30	376112.0
31	406874.0
32	437636.0
33	479234.5
34	520833.0
35	571141.5
36	621450.0
37	670081.5
38	718713.0
39	763014.0
40	807315.0
41	847296.5
42	921816.5
43	956355.0
44	984274.5
45	1012194.0
46	1025500.0
47	1038806.0
48	1040893.0
49	1042980.0
50	1037287.0
51	1031594.0
52	1005841.5
53	980089.0
54	935932.0
55	891775.0
56	829306.5
57	766838.0
58	696347.0
59	550012.0
60	474168.0
61	410920.5
62	347673.0
63	293199.5
64	238726.0
65	193069.0
66	147412.0
67	117608.0
68	87804.0
69	67556.5
70	47309.0
71	35917.5
72	24526.0
73	18249.5
74	11973.0
75	9107.5
76	4762.0
77	3282.0
78	2382.5
79	1483.0
80	1131.5
81	780.0
82	576.0
83	372.0
84	249.5
85	127.0
86	94.5
87	62.0
88	50.0
89	38.0
90	24.0
91	10.0
92	7.5
93	4.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.5203092E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.57375740430588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6337402082109	39.63896117490279
2	15.542804760259093	16.964585127377408
3	5.178611246475895	8.478488215691566
4	2.219513545333354	4.845087751143733
5	1.137473504398168	3.1038101541425633
6	0.6805858957687595	2.228527774108589
7	0.4669484554474277	1.7838192209532275
8	0.3304694996961051	1.4427969844750062
9	0.2565763049539409	1.2602099720024618
>10	1.433389721598575	13.996247024112687
>50	0.08830039774618881	3.321235274375816
>100	0.031218165741270405	2.4874466244882645
>500	2.2996783911808773E-4	0.07876233823481243
>1k	1.0060234673339094E-4	0.10453276260948127
>5k	2.5149456360135875E-5	0.11539982776727141
>10k+	1.2574728180067937E-5	0.15008977361429546
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21871	0.14385889396709564	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.577609344204455E-5	0.0	0.0	1.3155218688408911E-5	6.5776093442044555E-6
2	9.208653081886238E-5	0.0	0.0	1.9732828032613366E-5	6.5776093442044555E-6
3	9.866414016306682E-5	0.0	0.0	3.946565606522673E-5	6.5776093442044555E-6
4	9.866414016306682E-5	0.0	0.0	4.604326540943119E-5	6.5776093442044555E-6
5	5.788296222899921E-4	0.0	0.0	6.577609344204455E-5	1.9732828032613366E-5
6	5.854072316341966E-4	0.0	0.0	7.235370278624901E-5	3.946565606522673E-5
7	5.91984840978401E-4	0.0	0.0	9.866414016306682E-5	3.946565606522673E-5
8	6.051400596668099E-4	0.0	0.0	1.183969681956802E-4	5.2620874753635644E-5
9	6.051400596668099E-4	0.0	0.0	1.183969681956802E-4	5.2620874753635644E-5
10	6.380281063878321E-4	0.0	0.0	1.2497457753988465E-4	5.91984840978401E-5
11	6.446057157320366E-4	0.0	0.0	1.2497457753988465E-4	6.577609344204455E-5
12	7.36692246550899E-4	0.0	0.0	1.4470740557249803E-4	7.235370278624901E-5
13	8.287787773697614E-4	0.0	0.0	1.5786262426090692E-4	7.893131213045346E-5
14	8.81399652123397E-4	0.0	0.0	1.907506709819292E-4	8.550892147465791E-5
15	9.800637922864639E-4	6.5776093442044555E-6	0.0	1.907506709819292E-4	9.866414016306682E-5
16	0.001039262276384304	6.5776093442044555E-6	0.0	1.907506709819292E-4	1.1181935885147574E-4
17	0.0010655727137611217	6.5776093442044555E-6	0.0	2.1048349901454258E-4	1.1181935885147574E-4
18	0.0011181935885147573	6.5776093442044555E-6	0.0	2.2363871770295147E-4	1.1181935885147574E-4
19	0.0011642368539241887	6.5776093442044555E-6	0.0	2.2363871770295147E-4	1.2497457753988465E-4
20	0.001197124900645211	6.5776093442044555E-6	0.0	2.4337154573556485E-4	1.315521868840891E-4
21	0.0012629009940872554	6.5776093442044555E-6	0.0	2.4337154573556485E-4	1.315521868840891E-4
22	0.0012760562127756643	6.5776093442044555E-6	0.0	2.4337154573556485E-4	1.3812979622829355E-4
23	0.0012957890408082776	6.5776093442044555E-6	0.0	2.5652676442397375E-4	1.5786262426090692E-4
24	0.0013352546968735043	1.3155218688408911E-5	0.0	2.631043737681782E-4	1.5786262426090692E-4
25	0.0013944531809713446	1.3155218688408911E-5	0.0	2.631043737681782E-4	1.5786262426090692E-4
26	0.0014470740557249802	1.3155218688408911E-5	0.0	2.828372018007916E-4	1.5786262426090692E-4
27	0.0015128501491670247	1.9732828032613366E-5	0.0	2.828372018007916E-4	1.6444023360511137E-4
28	0.0015852038519532736	2.6310437376817822E-5	0.0	2.8941481114499605E-4	1.6444023360511137E-4
29	0.0016641351640837273	2.6310437376817822E-5	0.0	3.0257002983340495E-4	1.6444023360511137E-4
30	0.001703600820148954	2.6310437376817822E-5	0.0	3.091476391776094E-4	1.6444023360511137E-4
31	0.0017693769135909984	2.6310437376817822E-5	0.0	3.1572524852181385E-4	1.6444023360511137E-4
32	0.001835153007033043	3.2888046721022275E-5	0.0	3.2888046721022275E-4	1.9732828032613365E-4
33	0.00193381714719611	3.2888046721022275E-5	0.0	3.2888046721022275E-4	2.0390588967033813E-4
34	0.002019326068670768	3.2888046721022275E-5	0.0	3.4203568589863165E-4	2.1048349901454258E-4
35	0.0021771886929316748	4.604326540943119E-5	0.0	3.7492373261965395E-4	2.4337154573556485E-4
36	0.002354784145225195	4.604326540943119E-5	0.0	3.8807895130806285E-4	2.5652676442397375E-4
37	0.0024797587227650794	4.604326540943119E-5	0.0	3.946565606522673E-4	2.631043737681782E-4
38	0.0027099750498122354	4.604326540943119E-5	0.0	4.143893886848807E-4	2.959924204892005E-4
39	0.0028678376740731426	4.604326540943119E-5	0.0	4.2096699802908515E-4	2.959924204892005E-4
40	0.003058588345055072	5.2620874753635644E-5	0.0	4.275446073732896E-4	3.223028578660183E-4
41	0.003308537500134841	5.2620874753635644E-5	0.0	4.3412221671749405E-4	3.223028578660183E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	17300	0.0	28.716187	1
CAACGCA	24050	0.0	21.741163	13
TCAACGC	24025	0.0	21.734444	12
AACGCAG	24685	0.0	21.21997	14
AGTGGTA	24825	0.0	21.090836	5
GTATCAA	24900	0.0	21.0745	9
GGTATCA	24885	0.0	21.049427	8
ACGCAGA	25000	0.0	21.009	15
GTGGTAT	24875	0.0	20.972864	6
ATCAACG	25050	0.0	20.920158	11
TGGTATC	25425	0.0	20.57463	7
AGCAGTG	25665	0.0	20.492111	2
CGCAGAG	26105	0.0	20.128712	16
GCAGTGG	26260	0.0	19.974104	3
TATCAAC	26465	0.0	19.846022	10
CAGTGGT	27400	0.0	19.18595	4
AGAGTAC	26295	0.0	19.035938	19
CAGAGTA	27825	0.0	18.14124	18
GCAGAGT	28625	0.0	17.864105	17
ACATGGG	20240	0.0	17.253458	24
>>END_MODULE