FastQCFastQC Report
Thu 26 May 2016
SRR805398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16244286
Sequences flagged as poor quality0
Sequence length53
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1277950.786707399758906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT583050.041.0267561
TCAACGC661200.036.24160812
CAACGCA663100.036.1023213
AGTGGTA660000.035.9728055
TGGTATC661600.035.910677
ATCAACG668800.035.86490611
GGTATCA665100.035.8100288
AACGCAG670900.035.696614
GTGGTAT664550.035.694686
ACGCAGA673300.035.5693615
GTATCAA673000.035.3757069
CGCAGAG678250.035.3409516
AGCAGTG678250.035.2993742
GCAGTGG679800.035.288033
TATCAAC681950.034.95622610
CAGTGGT689300.034.535764
AGAGTAC683100.034.33662819
GAGTACT353000.033.69220720
CAGAGTA707100.033.57332618
GCAGAGT711300.033.46098717
Produced by FastQC (version 0.11.5)