FastQCFastQC Report
Thu 26 May 2016
SRR805397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16319771
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT234950.14396648090221364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT201350.029.6215551
TCAACGC267450.022.29182812
CAACGCA268950.022.16750513
AGCAGTG276900.021.658362
ATCAACG276150.021.60655411
AACGCAG276300.021.59482414
AGTGGTA276500.021.5452085
GTGGTAT275800.021.463566
GGTATCA277450.021.4544948
GTATCAA278950.021.2548839
TGGTATC279350.021.2328627
ACGCAGA280650.021.2098715
GCAGTGG291800.020.6812883
CGCAGAG289850.020.62584116
TATCAAC290500.020.4259910
AGAGTAC295000.019.72406819
CAGTGGT305450.019.5801284
GAGTACT123550.019.0396620
CAGAGTA322250.018.37703718
GTACTTT131300.017.91584222
Produced by FastQC (version 0.11.5)