FastQCFastQC Report
Thu 26 May 2016
SRR805396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13515618
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1237090.9153040578684599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT736850.042.1491471
TCAACGC756950.041.3745312
CAACGCA757550.041.3572713
GTGGTAT752900.041.0664756
GTATCAA757950.040.9757929
GGTATCA758600.040.9375848
ATCAACG763550.040.93071711
AGTGGTA756150.040.917945
AACGCAG766250.040.906114
ACGCAGA766450.040.86783215
TGGTATC757950.040.830077
CGCAGAG774050.040.4847916
TATCAAC771600.040.26004410
CAGTGGT776350.039.953184
AGAGTAC770300.039.76054819
GCAGTGG791350.039.573013
AGCAGTG792300.039.561152
CAGAGTA790950.039.08793318
GCAGAGT798600.038.7929517
GAGTACT311750.038.233220
Produced by FastQC (version 0.11.5)