FastQCFastQC Report
Thu 26 May 2016
SRR805395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14343375
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2006671.3990221966587362No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG308310.21494941044210306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG199880.13935353429719294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC143510.10005316043120954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1283950.044.340591
TCAACGC1324550.043.05775512
GTGGTAT1314750.043.0069246
CAACGCA1327100.042.98564513
AGTGGTA1317550.042.949415
GTATCAA1323150.042.9186069
GGTATCA1323300.042.8888788
TGGTATC1322300.042.8057947
ATCAACG1334200.042.74456411
AACGCAG1336700.042.6874814
ACGCAGA1335750.042.68440615
TATCAAC1336900.042.50179710
CGCAGAG1344200.042.42482416
AGCAGTG1347250.042.356692
GCAGTGG1352550.042.1959273
CAGTGGT1344200.042.1748244
AGAGTAC1326850.041.90628419
CAGAGTA1351600.041.67790218
GCAGAGT1361300.041.5293917
GTACATG895200.041.0068722