##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805395_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14343375
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.22153691164039	37.0	35.0	37.0	32.0	37.0
2	35.73717970840196	37.0	35.0	37.0	35.0	37.0
3	37.76688192283894	39.0	37.0	39.0	35.0	39.0
4	37.79701848414338	39.0	38.0	39.0	35.0	39.0
5	37.76217508083	39.0	37.0	39.0	35.0	39.0
6	37.669544789842	39.0	37.0	39.0	35.0	39.0
7	37.55730314518026	39.0	37.0	39.0	35.0	39.0
8	38.60020699451837	40.0	39.0	40.0	36.0	40.0
9	38.646986012706215	40.0	39.0	40.0	36.0	40.0
10	38.33156059853417	40.0	38.0	40.0	35.0	40.0
11	38.51233199996514	40.0	38.0	40.0	36.0	40.0
12	38.568326004165684	40.0	39.0	40.0	36.0	40.0
13	38.62833851865408	40.0	39.0	40.0	36.0	40.0
14	38.628965707164454	40.0	39.0	40.0	36.0	40.0
15	38.61600557748786	40.0	39.0	40.0	36.0	40.0
16	38.5740020044097	40.0	39.0	40.0	36.0	40.0
17	38.35864962046938	40.0	38.0	40.0	35.0	40.0
18	38.41729816030049	40.0	38.0	40.0	35.0	40.0
19	38.35876061247789	40.0	38.0	40.0	35.0	40.0
20	38.27612301846671	40.0	38.0	40.0	35.0	40.0
21	38.14171288138252	40.0	38.0	40.0	34.0	40.0
22	38.17883189974466	40.0	38.0	40.0	34.0	40.0
23	37.80029630404281	40.0	38.0	40.0	34.0	40.0
24	37.93528705761371	40.0	38.0	40.0	34.0	40.0
25	37.571458948817835	40.0	38.0	40.0	33.0	40.0
26	37.77526474766225	40.0	38.0	40.0	34.0	40.0
27	37.804417649263165	40.0	38.0	40.0	34.0	40.0
28	37.6690544589401	40.0	38.0	40.0	33.0	40.0
29	37.59929835202663	40.0	37.0	40.0	33.0	40.0
30	37.48350963423881	40.0	37.0	40.0	33.0	40.0
31	37.35818069317716	40.0	37.0	40.0	33.0	40.0
32	37.267232433091934	40.0	37.0	40.0	32.0	40.0
33	32.94276953645847	38.0	33.0	40.0	10.0	40.0
34	34.02545488770948	38.0	33.0	40.0	15.0	40.0
35	35.67559490008453	38.0	33.0	40.0	29.0	40.0
36	36.39662875717884	38.0	35.0	40.0	30.0	40.0
37	36.50308069056272	38.0	35.0	40.0	31.0	40.0
38	36.39145061744534	38.0	35.0	40.0	31.0	40.0
39	31.541702284155576	38.0	32.0	40.0	3.0	40.0
40	33.034343939275104	38.0	32.0	40.0	15.0	40.0
41	34.50927093518785	38.0	31.0	40.0	24.0	40.0
42	35.31222609741431	37.0	34.0	40.0	29.0	40.0
43	35.65006422825869	38.0	34.0	40.0	30.0	40.0
44	35.646322431087526	38.0	34.0	40.0	30.0	40.0
45	30.912256494723174	37.0	30.0	39.0	3.0	40.0
46	32.065360907038965	36.0	30.0	39.0	12.0	40.0
47	33.217699948582535	36.0	30.0	39.0	22.0	40.0
48	34.095717291083865	36.0	33.0	39.0	26.0	40.0
49	34.321241967110254	36.0	33.0	39.0	26.0	40.0
50	34.42637552180013	36.0	33.0	39.0	27.0	40.0
51	34.305057212824735	36.0	33.0	38.0	27.0	40.0
52	34.0497342501329	36.0	33.0	38.0	26.0	40.0
53	33.99856979267432	36.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	8.0
12	11.0
13	52.0
14	151.0
15	412.0
16	950.0
17	1907.0
18	3662.0
19	6549.0
20	11899.0
21	19192.0
22	29448.0
23	42673.0
24	58952.0
25	78098.0
26	101329.0
27	128721.0
28	162184.0
29	202882.0
30	256794.0
31	335610.0
32	468467.0
33	751469.0
34	1250686.0
35	880117.0
36	970344.0
37	1725715.0
38	3888373.0
39	2966720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.694095706205827	37.0285933401309	29.733511115759022	21.54379983790426
2	28.29684087601419	41.91279249130696	13.584947754625393	16.205418878053457
3	34.269681996043474	25.890747470522108	19.101536423610202	20.73803410982422
4	21.862162845215995	24.4000453170889	20.531192972365293	33.20659886532981
5	20.713263091845537	33.23132108028968	23.878097030859195	22.177318797005587
6	30.49903526889592	23.903188754390094	22.345835620974842	23.251940355739148
7	22.908422878158035	23.327536231884057	31.6229618203526	22.141079069605304
8	29.730729343686548	25.09907884301986	23.583647502766958	21.586544310526637
9	30.06766538558742	24.259388045003355	23.99603998361613	21.676906585793095
10	21.788735217478454	24.829002936895954	31.796073099950327	21.58618874567527
11	21.483597828265662	33.08081257026328	23.90169677638631	21.533892825084752
12	21.260644722737847	24.534734677159314	32.28008749684088	21.924533103261957
13	21.330886210532736	24.765384715940286	23.98299563387278	29.920733439654196
14	21.799318500701542	33.24856248965114	23.54339895596399	21.408720053683318
15	21.51251710284365	32.863667023974486	23.605818017028767	22.0179978561531
16	21.82200493259083	24.290454652409213	23.80201312452613	30.085527290473824
17	30.365036122948748	24.12268381744185	23.784590446809066	21.727689612800337
18	21.646209486958266	24.387579631711503	23.834404385299834	30.1318064960304
19	21.96823969254098	32.65856885147324	23.73727243413771	21.635919021848064
20	30.35549861870027	24.54114181634378	23.720372645907954	21.38298691904799
21	22.04539029342815	32.550525939675985	23.749598682318492	21.654485084577374
22	29.976382824823304	24.16663442181495	24.16746407313481	21.689518680226936
23	22.08013804282465	24.381144605087716	32.07724123506497	21.461476117022666
24	21.810069108560572	32.30916015233514	23.83500396524528	22.04576677385901
25	21.901128569810105	24.312262629959825	23.892751880223447	29.893856920006623
26	21.969759557984087	29.70616748150278	26.721563090974055	21.602509869539073
27	22.252817067112865	23.991605880763768	32.09093396777258	21.66464308435079
28	27.513461789850712	24.02739243727505	26.719687660679583	21.739458112194654
29	27.762942822034564	24.225818539918258	26.419277192432045	21.591961445615134
30	27.577303110320965	24.076474330483585	26.530687512527564	21.815535046667886
31	25.465812613837397	24.874933549460987	27.159054267213957	22.500199569487656
32	24.560683939449397	25.70480099697596	27.313369412707956	22.421145650866688
33	26.32627955414956	27.715122835455393	25.525721805363105	20.432875805031937
34	23.507312609479985	25.185481101902447	27.89083461876999	23.416371669847578
35	23.378751514200808	25.394629924965358	27.998884502427078	23.227734058406757
36	23.51265305411035	25.323851603963504	28.07100142051644	23.09249392140971
37	23.831782966003466	25.21486051922926	27.978164135010065	22.975192379757207
38	24.03135942551875	25.343100908956224	27.905252424899995	22.72028724062503
39	20.349617854933026	36.26542567561679	23.84707225461232	19.53788421483786
40	23.375600233557304	25.717573444185902	27.76326352758678	23.143562794670014
41	23.730983816570365	25.92476317463637	27.530403409239458	22.8138495995538
42	23.776558864284034	25.79029691408054	27.643814653106396	22.789329568529023
43	23.493877835586115	25.60107366641394	28.208814173791037	22.696234324208913
44	23.847846131053537	25.352617497625214	27.778985071505137	23.020551299816116
45	21.733281044384604	34.01717517669307	24.227652139053742	20.02189163986858
46	23.593484796988157	25.162404245862636	27.67232258795437	23.571788369194838
47	23.683003477215088	25.31261296591632	27.712710571953952	23.291672984914637
48	23.40966474069039	25.16941793685238	27.813223875133993	23.607693447323243
49	23.202398319781782	25.72454530401666	27.655464630883596	23.41759174531796
50	23.82325638143045	25.644097013429544	27.41038981411279	23.122256791027215
51	23.68355425414172	25.357351390450294	27.69946403827551	23.259630317132473
52	23.90023268582185	25.328453031451804	27.48064524562734	23.29066903709901
53	24.294707486905974	25.5370998806069	26.942633794347564	23.22555883813956
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3078.0
1	2010.0
2	942.0
3	782.0
4	622.0
5	635.5
6	649.0
7	725.0
8	992.0
9	1183.0
10	1779.5
11	2376.0
12	3582.5
13	4789.0
14	6375.5
15	7962.0
16	11502.0
17	15042.0
18	20880.5
19	26719.0
20	38923.0
21	51127.0
22	190480.0
23	329833.0
24	259558.5
25	190808.5
26	192333.0
27	207103.5
28	221874.0
29	243582.0
30	265290.0
31	285975.5
32	306661.0
33	342527.0
34	378393.0
35	423806.5
36	469220.0
37	517378.0
38	565536.0
39	612547.5
40	659559.0
41	705167.5
42	792906.0
43	835036.0
44	876044.5
45	917053.0
46	949688.0
47	982323.0
48	1011026.5
49	1039730.0
50	1045553.5
51	1051377.0
52	1038086.5
53	1024796.0
54	992376.0
55	959956.0
56	924277.0
57	888598.0
58	793272.0
59	610498.0
60	523050.0
61	450964.5
62	378879.0
63	317297.5
64	255716.0
65	207044.0
66	158372.0
67	125584.5
68	92797.0
69	71143.5
70	49490.0
71	36566.5
72	23643.0
73	17428.0
74	11213.0
75	8257.5
76	3740.0
77	2178.0
78	1561.5
79	945.0
80	768.0
81	591.0
82	400.5
83	210.0
84	142.5
85	75.0
86	49.5
87	24.0
88	18.5
89	13.0
90	10.0
91	7.0
92	5.5
93	2.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.4343375E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.81860734386925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.85382837433487	41.361660121514454
2	12.550120008779848	13.50859961741919
3	4.045456036275559	6.531624298296
4	1.9469197344374078	4.191220348708683
5	1.164131623069038	3.132597135926688
6	0.7211937534831668	2.3288186062537077
7	0.5233628545699481	1.971666197792661
8	0.36633896255647114	1.5772682224469714
9	0.27075055285266997	1.311427592290203
>10	1.4077529065352292	13.443863374290773
>50	0.10383979567728434	3.877002657400652
>100	0.04497652464721843	3.8204522419964233
>500	7.145120824994493E-4	0.2529091215875764
>1k	5.075198680341985E-4	0.5275658501051266
>5k	2.671020770834279E-5	0.0995401154845443
>10k+	8.013062312502836E-5	2.0637844984864717
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	200667	1.3990221966587362	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	30831	0.21494941044210306	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	19988	0.13935353429719294	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	14351	0.10005316043120954	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.788743932303241E-4	0.0	0.0	6.971859830758102E-6	2.0915579492274308E-5
2	0.007411087000095863	0.0	0.0	6.971859830758102E-6	4.880301881530672E-5
3	0.007432002579588137	0.0	0.0	6.971859830758102E-6	6.274673847682292E-5
4	0.007578411636034057	0.0	0.0	6.971859830758102E-6	9.063417779985534E-5
5	0.007578411636034057	0.0	0.0	6.971859830758102E-6	1.0457789746137153E-4
6	0.007578411636034057	0.0	0.0	6.971859830758102E-6	1.3246533678440395E-4
7	0.0075993272155263315	0.0	0.0	6.971859830758102E-6	1.4640905644592016E-4
8	0.00760629907535709	0.0	0.0	1.3943719661516205E-5	2.0218393509198498E-4
9	0.00760629907535709	0.0	0.0	1.3943719661516205E-5	2.788743932303241E-4
10	0.007620242795018606	0.0	0.0	1.3943719661516205E-5	2.858462530610822E-4
11	0.007627214654849364	6.971859830758102E-6	0.0	1.3943719661516205E-5	2.997899727225984E-4
12	0.007801511150618317	6.971859830758102E-6	0.0	1.3943719661516205E-5	3.067618325533565E-4
13	0.007892145328418173	6.971859830758102E-6	0.0	1.3943719661516205E-5	3.276774120456308E-4
14	0.007947920207064236	6.971859830758102E-6	0.0	2.0915579492274308E-5	3.5556485136866323E-4
15	0.008031582525033333	6.971859830758102E-6	0.0	2.0915579492274308E-5	3.5556485136866323E-4
16	0.008073413684017882	6.971859830758102E-6	0.0	2.788743932303241E-5	3.8345229069169565E-4
17	0.00808038554384864	6.971859830758102E-6	0.0	2.788743932303241E-5	4.0436787018396995E-4
18	0.008184963441310013	6.971859830758102E-6	0.0	2.788743932303241E-5	4.601427488300348E-4
19	0.008219822740463803	1.3943719661516205E-5	0.0	2.788743932303241E-5	4.810583283223091E-4
20	0.008226794600294561	1.3943719661516205E-5	0.0	2.788743932303241E-5	5.019739078145833E-4
21	0.008282569478940625	1.3943719661516205E-5	0.0	2.788743932303241E-5	5.159176274760996E-4
22	0.008331372497755932	2.0915579492274308E-5	0.0	6.274673847682292E-5	5.298613471376158E-4
23	0.00841503481572503	2.0915579492274308E-5	0.0	6.274673847682292E-5	5.577487864606482E-4
24	0.00852658457301716	2.0915579492274308E-5	0.0	1.2549347695364583E-4	5.577487864606482E-4
25	0.008589331311493982	2.0915579492274308E-5	0.0	1.2549347695364583E-4	5.716925061221644E-4
26	0.008700881068786113	2.0915579492274308E-5	0.0	1.3246533678440395E-4	5.856362257836807E-4
27	0.008798487106416725	2.0915579492274308E-5	0.0	1.3246533678440395E-4	5.995799454451968E-4
28	0.008896093144047339	2.0915579492274308E-5	0.0	1.4640905644592016E-4	6.06551805275955E-4
29	0.009021586621000985	2.0915579492274308E-5	0.0	1.4640905644592016E-4	6.344392445989873E-4
30	0.009133136378293114	2.0915579492274308E-5	0.0	1.7429649576895256E-4	6.623266839220198E-4
31	0.009391095192031164	2.0915579492274308E-5	0.0	1.7429649576895256E-4	6.623266839220198E-4
32	0.009523560528815568	2.0915579492274308E-5	0.0	1.8126835559971067E-4	6.832422634142941E-4
33	0.009746660043399828	2.0915579492274308E-5	0.0	1.8126835559971067E-4	7.250734223988427E-4
34	0.009865181660522715	2.788743932303241E-5	0.0	1.9521207526122688E-4	7.320452822296008E-4
35	0.010011590716968635	2.788743932303241E-5	0.0	1.9521207526122688E-4	7.529608617218751E-4
36	0.010206802792229863	2.788743932303241E-5	0.0	1.9521207526122688E-4	8.017638805371818E-4
37	0.010457789746137154	2.788743932303241E-5	0.0	2.0218393509198498E-4	8.226794600294561E-4
38	0.01068786112055217	2.788743932303241E-5	0.0	2.0218393509198498E-4	8.296513198602142E-4
39	0.010883073195813399	2.788743932303241E-5	0.0	2.0915579492274307E-4	8.575387591832466E-4
40	0.011120116430059173	2.788743932303241E-5	0.0	2.1612765475350119E-4	8.923980583370371E-4
41	0.011405962683120255	2.788743932303241E-5	0.0	2.1612765475350119E-4	9.063417779985533E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	128395	0.0	44.34059	1
TCAACGC	132455	0.0	43.057755	12
GTGGTAT	131475	0.0	43.006924	6
CAACGCA	132710	0.0	42.985645	13
AGTGGTA	131755	0.0	42.94941	5
GTATCAA	132315	0.0	42.918606	9
GGTATCA	132330	0.0	42.888878	8
TGGTATC	132230	0.0	42.805794	7
ATCAACG	133420	0.0	42.744564	11
AACGCAG	133670	0.0	42.68748	14
ACGCAGA	133575	0.0	42.684406	15
TATCAAC	133690	0.0	42.501797	10
CGCAGAG	134420	0.0	42.424824	16
AGCAGTG	134725	0.0	42.35669	2
GCAGTGG	135255	0.0	42.195927	3
CAGTGGT	134420	0.0	42.174824	4
AGAGTAC	132685	0.0	41.906284	19
CAGAGTA	135160	0.0	41.677902	18
GCAGAGT	136130	0.0	41.52939	17
GTACATG	89520	0.0	41.00687	22
>>END_MODULE