FastQCFastQC Report
Thu 26 May 2016
SRR805394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14207239
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT505590.355867878340049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT347300.038.6501581
TCAACGC384400.034.79760712
CAACGCA386950.034.55614513
ATCAACG391850.034.1600111
ACGCAGA392300.034.13879815
AACGCAG392450.034.11377314
GTGGTAT391850.033.9201246
GGTATCA393400.033.8880278
GTATCAA394700.033.7942739
AGTGGTA394800.033.6547625
TGGTATC398300.033.4003267
CGCAGAG403550.033.19291316
TATCAAC404750.032.96096410
GCAGTGG412900.032.4924933
AGCAGTG413500.032.4396632
AGAGTAC406250.032.30707619
CAGTGGT419550.031.7366244
CAGAGTA424550.031.290918
GCAGAGT433300.030.72951717
GAGTACT167650.029.91291420
Produced by FastQC (version 0.11.5)