##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805394_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14207239
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.76749845624474	35.0	35.0	37.0	32.0	37.0
2	35.415106833917555	37.0	35.0	37.0	33.0	37.0
3	37.4965649553724	39.0	37.0	39.0	35.0	39.0
4	37.659064720456946	39.0	37.0	39.0	35.0	39.0
5	37.65009499734607	39.0	37.0	39.0	35.0	39.0
6	37.515868494927126	39.0	37.0	39.0	35.0	39.0
7	37.55816172304837	39.0	37.0	39.0	35.0	39.0
8	38.56760662645289	40.0	39.0	40.0	36.0	40.0
9	38.58775860672154	40.0	39.0	40.0	36.0	40.0
10	38.432994264402815	40.0	38.0	40.0	35.0	40.0
11	38.52508119276377	40.0	39.0	40.0	36.0	40.0
12	38.49984321373069	40.0	38.0	40.0	36.0	40.0
13	38.526626742887906	40.0	39.0	40.0	35.0	40.0
14	38.464792209098476	40.0	39.0	40.0	35.0	40.0
15	38.472224758096914	40.0	39.0	40.0	35.0	40.0
16	38.42416974895685	40.0	38.0	40.0	35.0	40.0
17	38.185418433518294	40.0	38.0	40.0	34.0	40.0
18	38.22824885257438	40.0	38.0	40.0	34.0	40.0
19	38.16613354642658	40.0	38.0	40.0	34.0	40.0
20	38.09133280576191	40.0	38.0	40.0	34.0	40.0
21	38.03215325651944	40.0	38.0	40.0	34.0	40.0
22	37.960208595069034	40.0	38.0	40.0	34.0	40.0
23	37.80592217812342	40.0	38.0	40.0	34.0	40.0
24	37.79724603774174	40.0	38.0	40.0	34.0	40.0
25	37.474036440155615	40.0	37.0	40.0	33.0	40.0
26	37.58134884617623	40.0	37.0	40.0	33.0	40.0
27	37.56482607211718	40.0	37.0	40.0	33.0	40.0
28	37.47682874906236	40.0	37.0	40.0	33.0	40.0
29	37.38824665369535	40.0	37.0	40.0	33.0	40.0
30	37.28054233479144	40.0	37.0	40.0	32.0	40.0
31	37.184318642066906	39.0	37.0	40.0	32.0	40.0
32	37.10070675942032	39.0	37.0	40.0	32.0	40.0
33	32.578841392053725	38.0	33.0	40.0	10.0	40.0
34	33.779227617695454	38.0	33.0	40.0	16.0	40.0
35	35.46123493804813	38.0	33.0	40.0	28.0	40.0
36	36.18404026285473	38.0	35.0	40.0	30.0	40.0
37	36.348442931100124	38.0	35.0	40.0	30.0	40.0
38	36.32530155929664	38.0	35.0	40.0	30.0	40.0
39	31.20947616915574	38.0	31.0	40.0	3.0	40.0
40	32.82624027089289	38.0	31.0	40.0	15.0	40.0
41	34.38663409547767	37.0	31.0	40.0	24.0	40.0
42	35.15415599047781	37.0	34.0	40.0	28.0	40.0
43	35.41630594093617	38.0	34.0	39.0	29.0	40.0
44	35.47827477245931	38.0	34.0	39.0	29.0	40.0
45	30.632705974749914	37.0	30.0	39.0	3.0	40.0
46	31.87008658051012	36.0	30.0	39.0	12.0	40.0
47	33.05367707265289	36.0	30.0	39.0	22.0	40.0
48	34.01766740180833	36.0	32.0	39.0	26.0	40.0
49	34.223901491345366	36.0	33.0	38.0	26.0	40.0
50	34.34860214570896	36.0	33.0	38.0	26.0	40.0
51	34.2815525240337	36.0	33.0	38.0	27.0	40.0
52	34.09095222512974	36.0	33.0	38.0	26.0	40.0
53	34.068116049853174	36.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	19.0
10	38.0
11	62.0
12	118.0
13	299.0
14	544.0
15	1173.0
16	2026.0
17	3489.0
18	6208.0
19	10024.0
20	16166.0
21	25113.0
22	37241.0
23	51915.0
24	69376.0
25	90917.0
26	115600.0
27	144060.0
28	177134.0
29	219678.0
30	272440.0
31	348502.0
32	472459.0
33	753421.0
34	1265554.0
35	859605.0
36	910423.0
37	1637581.0
38	4015452.0
39	2700601.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.34142833804654	29.933191100677618	19.585212862259866	36.140167699015976
2	24.260639241727404	25.903470758815278	27.703236357183826	22.132653642273493
3	18.272536979211797	24.157726916538817	30.86556085950268	26.704175244746708
4	17.711703167659813	27.098945826138348	30.29682966549658	24.892521340705258
5	22.99925411263934	27.99645307578763	28.743938213469907	20.260354598103124
6	23.46724089036582	25.444739825943664	29.351811425147417	21.7362078585431
7	23.500576009173916	26.547262279461897	28.98290793869238	20.969253772671806
8	22.897862139153148	25.11501355048648	28.810326904474543	23.17679740588583
9	22.83814610284236	24.542228085273994	30.09109651776816	22.528529294115486
10	22.505477665294432	25.7152286943297	28.813311298557025	22.965982341818844
11	22.192679379856987	27.956149678343557	27.035013629319533	22.816157312479927
12	22.197233396298888	26.080056793582486	28.58848929056518	23.13422051955345
13	22.510010565740465	26.446201123244283	26.162458448119303	24.881329862895953
14	22.850717159048283	28.393342295431225	26.0288153102795	22.72712523524099
15	22.46427331869338	28.122987161685675	26.185545270266797	23.22719424935415
16	22.9964949558461	26.181202413783566	25.93646098302422	24.885841647346115
17	25.42248356630025	25.803866606312457	25.90474475723256	22.868905070154728
18	22.762649378953924	26.159685213995488	26.002955254008185	25.0747101530424
19	23.366925832668827	27.809407584401164	25.812854981886346	23.010811601043667
20	24.98890882317106	26.118311939427496	25.997697371037397	22.89508186636404
21	23.190508725868554	27.81219489585556	26.096653966333644	22.90064241194225
22	24.808831610420576	25.768947787814366	26.20497198646408	23.217248615300974
23	23.1042709987493	26.10341812367625	28.05726714388348	22.735043733690972
24	22.768836365742846	27.77957772090693	26.17294605939972	23.278639853950512
25	23.111316702703462	25.86135138572667	26.02132617041214	25.00600574115773
26	23.021102129695993	26.99514662912336	27.170669825431947	22.81308141574869
27	23.172025190819976	25.651704740097635	28.209696479379282	22.966573589703106
28	24.378572078642442	25.707465046516077	26.702204418465826	23.211758456375655
29	24.565293791425624	25.77180548592165	26.755487114702582	22.907413607950144
30	24.21103776743673	25.722936032821014	26.912773129247704	23.153253070494557
31	23.938887774042513	25.88061621262231	26.697333662085924	23.48316235124925
32	23.77901153067109	26.192985139477138	26.81088844919129	23.217114880660485
33	25.428135614527214	29.02239485096295	24.81565911575078	20.733810418759056
34	23.524493393825498	26.02211450092449	26.76187822278488	23.691513882465127
35	23.580211468252205	26.11161816873778	26.919192391991153	23.38897797101886
36	23.42814110468614	26.010697785825943	27.019437063035262	23.541724046452657
37	23.603467218366635	26.0334960226966	26.736025205178855	23.627011553757914
38	23.84417549391546	26.141898506810506	26.843076265557297	23.170849733716732
39	19.884018281103035	37.54341712700124	22.78532091984938	19.787243672046344
40	23.625322274088585	26.108098836093347	26.708982653139007	23.557596236679064
41	23.645319122174264	26.217944246591472	26.85503495788309	23.281701673351172
42	23.607627069552358	25.988624531480042	27.04914726921959	23.354601129748012
43	23.604368167523614	26.045954460257903	26.79659995865488	23.553077413563607
44	23.67000372134234	26.084484114049182	26.890439444286113	23.355072720322365
45	21.195659480353644	35.493356590960424	23.247057362799346	20.06392656588659
46	23.566823926872775	25.968859959348894	26.712558295105755	23.75175781867258
47	23.74189664860287	26.04983276483207	26.79506552962191	23.413205056943156
48	23.432026447925598	25.964348174898728	26.976810906045856	23.626814471129823
49	23.646466424616353	26.053359136141797	26.618761041466254	23.6814133977756
50	23.72542617182691	26.155011540243674	26.699818310932898	23.419743976996514
51	23.583181785004108	25.969577903208357	26.876707008307527	23.57053330348001
52	23.782981337894014	26.011450923011854	26.482837376072858	23.722730363021274
53	23.87611695699636	26.162810381383743	26.469949579928937	23.491123081690958
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3244.0
1	2366.0
2	1488.0
3	1259.5
4	1031.0
5	1021.0
6	1011.0
7	1197.5
8	1689.5
9	1995.0
10	2880.0
11	3765.0
12	5664.0
13	7563.0
14	9888.5
15	12214.0
16	17226.0
17	22238.0
18	30940.0
19	39642.0
20	53631.0
21	67620.0
22	119885.5
23	172151.0
24	168692.0
25	184970.5
26	204708.0
27	229168.5
28	253629.0
29	281952.0
30	310275.0
31	339399.5
32	368524.0
33	405299.5
34	442075.0
35	486819.0
36	531563.0
37	575277.0
38	618991.0
39	662789.0
40	706587.0
41	750316.0
42	832501.5
43	870958.0
44	905609.5
45	940261.0
46	964088.0
47	987915.0
48	1000023.5
49	1012132.0
50	1012073.5
51	1012015.0
52	994112.0
53	976209.0
54	933245.5
55	890282.0
56	833949.5
57	777617.0
58	705987.0
59	558194.5
60	482032.0
61	414676.0
62	347320.0
63	289788.0
64	232256.0
65	188121.5
66	143987.0
67	113690.5
68	83394.0
69	64088.5
70	44783.0
71	33228.0
72	21673.0
73	15953.5
74	10234.0
75	7557.5
76	3576.0
77	2271.0
78	1614.0
79	957.0
80	702.5
81	448.0
82	325.5
83	203.0
84	125.5
85	48.0
86	30.5
87	13.0
88	10.5
89	8.0
90	7.5
91	7.0
92	3.5
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.4207239E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.843224777019365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3394568651205	39.83825325189401
2	16.807097129711522	18.771250057273548
3	5.1457672945457515	8.620687190386596
4	2.255247942596871	5.037612711453901
5	1.2065720904881794	3.3689438229404773
6	0.7393761835198772	2.477349024664514
7	0.5021816653810013	1.9630410533138423
8	0.3525213151947824	1.574874163449018
9	0.25536661646573056	1.2834445827458416
>10	1.3217472647337098	12.853076791440433
>50	0.0602138405434545	2.2441650257960637
>100	0.014108738824357422	1.2310043984227677
>500	1.8630688881886883E-4	0.07418815061909376
>1k	1.1399755192907974E-4	0.15680099723221957
>5k	2.8498955649444297E-5	0.10299221313366552
>10k+	1.4249477824722149E-5	0.402316565234103
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50559	0.355867878340049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.771705536874547E-4	2.1115995866614196E-5	0.0	0.0	1.4077330577742796E-5
2	6.334798759984259E-4	2.1115995866614196E-5	0.0	0.0	1.4077330577742796E-5
3	6.405185412872973E-4	3.519332644435699E-5	0.0	0.0	1.4077330577742796E-5
4	6.405185412872973E-4	3.519332644435699E-5	0.0	0.0	1.4077330577742796E-5
5	0.001273998417285723	4.223199173322839E-5	0.0	0.0	2.8154661155485593E-5
6	0.0012880757478634659	4.927065702209979E-5	0.0	0.0	7.038665288871399E-5
7	0.0012880757478634659	5.6309322310971186E-5	0.0	0.0	7.038665288871399E-5
8	0.0012880757478634659	5.6309322310971186E-5	0.0	0.0	7.742531817758538E-5
9	0.0012880757478634659	5.6309322310971186E-5	0.0	0.0	7.742531817758538E-5
10	0.0013021530784412087	5.6309322310971186E-5	0.0	0.0	8.446398346645679E-5
11	0.0013303077395966944	5.6309322310971186E-5	0.0	0.0	8.446398346645679E-5
12	0.0013514237354633086	6.334798759984259E-5	0.0	0.0	9.854131404419958E-5
13	0.0014640423800852509	6.334798759984259E-5	0.0	0.0	1.0557997933307098E-4
14	0.0014781197106629937	6.334798759984259E-5	0.0	0.0	1.0557997933307098E-4
15	0.001520351702396222	7.038665288871399E-5	0.0	0.0	1.0557997933307098E-4
16	0.0015273903676850935	7.038665288871399E-5	0.0	0.0	1.1261864462194237E-4
17	0.0015273903676850935	7.038665288871399E-5	7.038665288871398E-6	7.038665288871398E-6	1.2669597519968518E-4
18	0.0015485063635517077	7.038665288871399E-5	7.038665288871398E-6	7.038665288871398E-6	1.2669597519968518E-4
19	0.0015625836941294505	8.446398346645679E-5	7.038665288871398E-6	7.038665288871398E-6	1.4077330577742797E-4
20	0.0015766610247071934	8.446398346645679E-5	7.038665288871398E-6	7.038665288871398E-6	1.4077330577742797E-4
21	0.001590738355284936	8.446398346645679E-5	7.038665288871398E-6	1.4077330577742796E-5	1.4077330577742797E-4
22	0.001590738355284936	8.446398346645679E-5	7.038665288871398E-6	2.8154661155485593E-5	1.4077330577742797E-4
23	0.0016470476775959073	8.446398346645679E-5	7.038665288871398E-6	2.8154661155485593E-5	1.4077330577742797E-4
24	0.00166112500817365	9.854131404419958E-5	7.038665288871398E-6	4.223199173322839E-5	1.6892796693291357E-4
25	0.0016892796693291356	9.854131404419958E-5	7.038665288871398E-6	4.223199173322839E-5	1.6892796693291357E-4
26	0.0017103956651957498	9.854131404419958E-5	7.038665288871398E-6	4.223199173322839E-5	1.6892796693291357E-4
27	0.001766704987506721	9.854131404419958E-5	7.038665288871398E-6	4.223199173322839E-5	1.7596663222178496E-4
28	0.0017878209833733353	9.854131404419958E-5	7.038665288871398E-6	4.927065702209979E-5	1.7596663222178496E-4
29	0.0018652463015509206	1.1261864462194237E-4	7.038665288871398E-6	4.927065702209979E-5	1.8300529751065636E-4
30	0.0018863622974175348	1.1261864462194237E-4	7.038665288871398E-6	7.038665288871399E-5	1.9004396279952775E-4
31	0.0019708262808839916	1.1261864462194237E-4	7.038665288871398E-6	7.742531817758538E-5	1.9004396279952775E-4
32	0.002083444925505934	1.1965730991081378E-4	7.038665288871398E-6	7.742531817758538E-5	1.9004396279952775E-4
33	0.002189024904839005	1.1965730991081378E-4	7.038665288871398E-6	7.742531817758538E-5	2.0412129337727056E-4
34	0.002308682214749819	1.1965730991081378E-4	7.038665288871398E-6	7.742531817758538E-5	2.0412129337727056E-4
35	0.002421300859371761	1.1965730991081378E-4	7.038665288871398E-6	7.742531817758538E-5	2.1115995866614196E-4
36	0.0026465381486156458	1.1965730991081378E-4	7.038665288871398E-6	7.742531817758538E-5	2.4635328511049895E-4
37	0.0027873114543930737	1.1965730991081378E-4	7.038665288871398E-6	8.446398346645679E-5	2.4635328511049895E-4
38	0.002878814103148402	1.2669597519968518E-4	7.038665288871398E-6	8.446398346645679E-5	2.745079462659845E-4
39	0.0030336647395035727	1.3373464048855658E-4	7.038665288871398E-6	9.150264875532818E-5	2.745079462659845E-4
40	0.0031744380452810006	1.3373464048855658E-4	7.038665288871398E-6	9.150264875532818E-5	2.745079462659845E-4
41	0.0033433660122139143	1.4077330577742797E-4	7.038665288871398E-6	9.854131404419958E-5	2.745079462659845E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	34730	0.0	38.650158	1
TCAACGC	38440	0.0	34.797607	12
CAACGCA	38695	0.0	34.556145	13
ATCAACG	39185	0.0	34.16001	11
ACGCAGA	39230	0.0	34.138798	15
AACGCAG	39245	0.0	34.113773	14
GTGGTAT	39185	0.0	33.920124	6
GGTATCA	39340	0.0	33.888027	8
GTATCAA	39470	0.0	33.794273	9
AGTGGTA	39480	0.0	33.654762	5
TGGTATC	39830	0.0	33.400326	7
CGCAGAG	40355	0.0	33.192913	16
TATCAAC	40475	0.0	32.960964	10
GCAGTGG	41290	0.0	32.492493	3
AGCAGTG	41350	0.0	32.439663	2
AGAGTAC	40625	0.0	32.307076	19
CAGTGGT	41955	0.0	31.736624	4
CAGAGTA	42455	0.0	31.2909	18
GCAGAGT	43330	0.0	30.729517	17
GAGTACT	16765	0.0	29.912914	20
>>END_MODULE