FastQCFastQC Report
Thu 26 May 2016
SRR805393_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805393_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17187137
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2413611.4043118408842612No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG396340.2306026885105995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG358380.20851640386644965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1566950.044.550941
TCAACGC1629800.043.13716512
CAACGCA1633400.043.05935313
AGTGGTA1618800.042.984625
GGTATCA1627050.042.9602058
GTATCAA1628300.042.934449
ATCAACG1637050.042.93320511
AGCAGTG1634200.042.8915982
GTGGTAT1622550.042.8794826
ACGCAGA1642750.042.7885215
AACGCAG1644350.042.78547314
TGGTATC1630050.042.736977
GCAGTGG1646550.042.636973
TATCAAC1641450.042.60909310
CGCAGAG1654950.042.50433316
AGAGTAC1628700.042.1865919
CAGTGGT1654800.042.1034554
CAGAGTA1660300.041.8040418
GAGTACT553500.041.6291820
GCAGAGT1675700.041.56710417