##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805393_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17187137
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.00434516813359	36.0	35.0	37.0	32.0	37.0
2	35.49139056725969	37.0	35.0	37.0	33.0	37.0
3	37.621292888978545	39.0	37.0	39.0	35.0	39.0
4	37.64974812268035	39.0	37.0	39.0	35.0	39.0
5	37.66036856516592	39.0	37.0	39.0	35.0	39.0
6	37.60740477020693	39.0	37.0	39.0	35.0	39.0
7	37.49518788382265	39.0	37.0	39.0	35.0	39.0
8	38.55125754801396	40.0	39.0	40.0	36.0	40.0
9	38.59469509086941	40.0	39.0	40.0	36.0	40.0
10	38.26167447201939	40.0	38.0	40.0	34.0	40.0
11	38.44980260528557	40.0	38.0	40.0	35.0	40.0
12	38.476886464569404	40.0	38.0	40.0	35.0	40.0
13	38.56025997814528	40.0	39.0	40.0	36.0	40.0
14	38.55247467917432	40.0	39.0	40.0	36.0	40.0
15	38.49075282288144	40.0	39.0	40.0	35.0	40.0
16	38.479295417264666	40.0	39.0	40.0	35.0	40.0
17	38.24320461284506	40.0	38.0	40.0	34.0	40.0
18	38.304466241236106	40.0	38.0	40.0	34.0	40.0
19	38.284776923579535	40.0	38.0	40.0	34.0	40.0
20	38.20195643986546	40.0	38.0	40.0	34.0	40.0
21	38.086734166371045	40.0	38.0	40.0	34.0	40.0
22	38.113936544521636	40.0	38.0	40.0	34.0	40.0
23	37.72936248777211	40.0	38.0	40.0	34.0	40.0
24	37.86448790162085	40.0	38.0	40.0	34.0	40.0
25	37.52966477197453	40.0	38.0	40.0	33.0	40.0
26	37.699491020523084	40.0	38.0	40.0	33.0	40.0
27	37.72986431655255	40.0	38.0	40.0	33.0	40.0
28	37.60624076016849	40.0	37.0	40.0	33.0	40.0
29	37.52940958113035	40.0	37.0	40.0	33.0	40.0
30	37.40869948264216	40.0	37.0	40.0	33.0	40.0
31	37.289750294071666	40.0	37.0	40.0	32.0	40.0
32	37.11183165643004	39.0	37.0	40.0	32.0	40.0
33	32.800422083096215	38.0	33.0	40.0	10.0	40.0
34	33.90934220167094	38.0	33.0	40.0	16.0	40.0
35	35.54017460848773	38.0	33.0	40.0	28.0	40.0
36	36.27974763917923	38.0	35.0	40.0	30.0	40.0
37	36.411500938172544	38.0	35.0	40.0	31.0	40.0
38	36.27182421365467	38.0	35.0	40.0	30.0	40.0
39	31.340047210888002	38.0	31.0	40.0	3.0	40.0
40	32.90302730466395	38.0	31.0	40.0	15.0	40.0
41	34.39008015122007	37.0	31.0	40.0	24.0	40.0
42	35.209010203386406	37.0	34.0	40.0	28.0	40.0
43	35.507567258002304	38.0	34.0	40.0	29.0	40.0
44	35.49060067421351	38.0	34.0	39.0	29.0	40.0
45	30.641396179014574	36.0	30.0	39.0	3.0	40.0
46	31.903878406275577	36.0	30.0	39.0	12.0	40.0
47	33.10873893656634	36.0	30.0	39.0	22.0	40.0
48	33.98900282228506	36.0	32.0	39.0	26.0	40.0
49	34.23513468240813	36.0	33.0	38.0	26.0	40.0
50	34.31514603042962	36.0	33.0	38.0	26.0	40.0
51	34.20026831693958	36.0	33.0	38.0	26.0	40.0
52	33.96141015225514	36.0	33.0	38.0	26.0	40.0
53	33.87329832769704	36.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	17.0
13	46.0
14	174.0
15	529.0
16	1187.0
17	2359.0
18	4746.0
19	8901.0
20	15772.0
21	25376.0
22	39018.0
23	55940.0
24	77127.0
25	103035.0
26	132856.0
27	167328.0
28	208059.0
29	260050.0
30	326412.0
31	422702.0
32	580820.0
33	912270.0
34	1510974.0
35	1076717.0
36	1188005.0
37	2083885.0
38	4613687.0
39	3369140.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.132336293124329	34.4307664505147	29.35200318703458	22.08489406932638
2	25.590376105106976	36.646138330077896	18.687085580338366	19.07639998447676
3	32.51928462547311	24.666295497615458	20.861880602918333	21.952539273993104
4	21.778909425112513	23.795301102213827	22.171790450032486	32.253999022641175
5	22.289465662605703	32.58152884916202	23.765342651309524	21.363662836922753
6	31.409134633650737	23.80480239379019	22.480876250651868	22.3051867219072
7	22.782217887714516	23.440326332419414	32.42916490396277	21.3482908759033
8	30.47118318775256	24.64287682119483	23.520060380038863	21.36587961101375
9	30.160555536387474	24.10322324189305	24.162744498982	21.573476722737475
10	21.660349830224778	24.447213052412394	32.33394252922985	21.558494588132973
11	21.503523245320032	32.90517786644745	24.004626250433684	21.586672637798838
12	21.319304081884027	24.32926437951824	32.51573545960563	21.835696078992097
13	21.307900204670503	24.464295595013876	23.98131812180237	30.24648607851325
14	21.705843154680153	33.04762742043658	23.737368242308186	21.509161182575085
15	21.56472599246751	32.77494093402525	23.717679099200755	21.942653974306484
16	21.67249845044	24.09346594490985	23.961396246506908	30.272639358143245
17	30.688793601866326	23.897743993080407	23.825870475111707	21.58759192994156
18	21.723367888438894	24.150432966235154	23.845466525343927	30.280732619982025
19	21.944416920630818	32.68237752454059	23.840363872121344	21.53284168270725
20	30.507925781937967	24.112637258898907	23.903632117437592	21.47580484172553
21	21.85010220143122	32.59123378140292	23.87582643927258	21.68283757789328
22	30.221513914737514	23.911015546102877	24.199097266752457	21.66837327240715
23	21.94772753600556	24.076075032159224	32.458861531155534	21.517335900679676
24	21.745977820506116	32.410854699069425	23.90017604444533	21.942991435979128
25	21.841089647449717	24.071048016897752	24.004858982621712	30.08300335303082
26	22.00499710917531	29.588418361941258	26.85426898034268	21.552315548540747
27	22.195069487140294	23.832526615689396	32.35684337653211	21.615560520638198
28	27.67212479891212	23.88475171868357	26.825538191730246	21.617585290674064
29	27.834036582125343	23.95797508334285	26.625405964937617	21.582582369594192
30	27.628842430243033	23.908734770660175	26.616573778401836	21.845849020694956
31	25.484936787319494	24.76756309093248	27.267217338175637	22.480282783572388
32	24.609241201719634	25.473818007036307	27.433801220063586	22.483139571180473
33	26.406829712243525	27.492339183658103	25.665787152333746	20.435043951764627
34	23.603419231486896	25.000272005744762	28.03152148027912	23.364787282489225
35	23.379490138468086	25.069847293356656	28.26829739007724	23.282365178098015
36	23.466177060204966	25.15076827513506	28.294793949684582	23.08826071497539
37	23.958458002633016	25.039981935327564	28.088302315854	22.913257746185415
38	24.08570432643901	25.14333248172747	28.057686396518513	22.713276795315007
39	20.285682251790977	36.357649328099264	23.900484414594473	19.456184005515286
40	23.54076772646893	25.581549736875896	27.848948897073434	23.02873363958174
41	23.78952934395065	25.725593506353032	27.672502988717667	22.812374160978642
42	23.839491126416227	25.663680926032068	27.755757110681085	22.74107083687062
43	23.535793075949766	25.48565243879769	28.269973061831067	22.70858142342148
44	23.794695998524944	25.16745517301689	28.049593134679736	22.988255693778434
45	21.719696538172702	34.190266825708086	24.15935824564615	19.930678390473062
46	23.7434716439393	25.01467230987918	27.754034892489653	23.487821153691858
47	23.65352065326529	25.06682759321695	27.90657338682993	23.37307836668783
48	23.36364107646317	25.089484071721778	27.966053915785977	23.580820936029077
49	23.23410234060507	25.605323329883273	27.728719448736577	23.431854880775084
50	23.858668258709987	25.534485470151314	27.47394170419425	23.132904566944454
51	23.784758334096015	25.25190204744397	27.728748540260078	23.234591078199934
52	23.967080730199566	25.227604807013527	27.59973345182505	23.205581010961858
53	24.436606282942876	25.342801421784213	27.045609748732442	23.174982546540473
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3332.0
1	2243.5
2	1155.0
3	969.5
4	784.0
5	756.0
6	728.0
7	829.0
8	1152.5
9	1375.0
10	1949.5
11	2524.0
12	4008.0
13	5492.0
14	7411.5
15	9331.0
16	13552.0
17	17773.0
18	24747.0
19	31721.0
20	45928.0
21	60135.0
22	226470.5
23	392806.0
24	310256.5
25	227936.0
26	228165.0
27	244215.0
28	260265.0
29	285341.0
30	310417.0
31	337485.0
32	364553.0
33	406662.5
34	448772.0
35	501887.0
36	555002.0
37	611056.0
38	667110.0
39	723582.5
40	780055.0
41	837809.5
42	950271.0
43	1004978.0
44	1058215.0
45	1111452.0
46	1150850.5
47	1190249.0
48	1219015.5
49	1247782.0
50	1257705.0
51	1267628.0
52	1252703.0
53	1237778.0
54	1198373.0
55	1158968.0
56	1117932.5
57	1076897.0
58	952976.0
59	722949.0
60	616843.0
61	533416.0
62	449989.0
63	378291.0
64	306593.0
65	248389.0
66	190185.0
67	151420.5
68	112656.0
69	86748.0
70	60840.0
71	45709.0
72	30578.0
73	22777.5
74	14977.0
75	11227.5
76	5535.0
77	3592.0
78	2567.0
79	1542.0
80	1206.0
81	870.0
82	621.0
83	372.0
84	235.0
85	98.0
86	59.5
87	21.0
88	16.5
89	12.0
90	7.5
91	3.0
92	2.5
93	2.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7187137E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.63532000616582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.24849006440505	41.658606546557245
2	13.1470779018449	14.365896166265744
3	4.232065143734142	6.936607002445649
4	1.7947861801019391	3.9223486917005363
5	1.0433430003862973	2.850168935114927
6	0.6473194522968666	2.121990325347318
7	0.48751561414566313	1.8644900110795535
8	0.35910086914821837	1.5695672720324152
9	0.2979056549434936	1.4648553710535765
>10	1.6423353178778952	15.52370017880923
>50	0.07698207009884817	2.856593147129355
>100	0.02196984187147846	1.9271143751550293
>500	5.756834798209882E-4	0.21680316459905627
>1k	4.4616887776341695E-4	0.4940329788713043
>5k	1.0879598448441698E-5	0.05538715573923379
>10k+	7.615718913909188E-5	2.17183867809981
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	241361	1.4043118408842612	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	39634	0.2306026885105995	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	35838	0.20851640386644965	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1527727392875264E-4	0.0	0.0	0.0	0.0
2	3.200067585427404E-4	0.0	0.0	0.0	5.818304700777099E-6
3	3.200067585427404E-4	0.0	0.0	0.0	1.1636609401554198E-5
4	3.2582506324351754E-4	0.0	0.0	0.0	2.3273218803108396E-5
5	3.4327997734584883E-4	0.0	0.0	0.0	3.490982820466259E-5
6	3.7237150084973433E-4	0.0	0.0	0.0	3.490982820466259E-5
7	3.781898055505114E-4	0.0	0.0	0.0	3.490982820466259E-5
8	3.840081102512885E-4	0.0	0.0	0.0	3.490982820466259E-5
9	3.898264149520656E-4	0.0	0.0	0.0	4.654643760621679E-5
10	3.898264149520656E-4	0.0	0.0	0.0	5.818304700777099E-5
11	4.014630243536198E-4	0.0	0.0	0.0	6.981965640932519E-5
12	4.189179384559511E-4	0.0	0.0	0.0	6.981965640932519E-5
13	6.283769076839267E-4	0.0	0.0	0.0	6.981965640932519E-5
14	6.749233452901434E-4	0.0	0.0	5.818304700777099E-6	6.981965640932519E-5
15	8.84382314518119E-4	0.0	0.0	5.818304700777099E-6	8.145626581087938E-5
16	9.483836662266671E-4	0.0	0.0	5.818304700777099E-6	9.891117991321067E-5
17	9.658385803289984E-4	0.0	0.0	5.818304700777099E-6	9.891117991321067E-5
18	0.0011054778931476488	0.0	0.0	5.818304700777099E-6	9.891117991321067E-5
19	0.0011578426354546425	5.818304700777099E-6	0.0	5.818304700777099E-6	1.0472948461398778E-4
20	0.0011985707683600823	5.818304700777099E-6	0.0	5.818304700777099E-6	1.1636609401554198E-4
21	0.0012509355106670762	5.818304700777099E-6	0.0	5.818304700777099E-6	1.1636609401554198E-4
22	0.001297481948273293	5.818304700777099E-6	0.0	1.7454914102331297E-5	1.1636609401554198E-4
23	0.0013265734717771785	5.818304700777099E-6	0.0	1.7454914102331297E-5	1.2218439871631908E-4
24	0.0014138480422888349	5.818304700777099E-6	0.0	7.563796111010228E-5	1.2800270341709616E-4
25	0.0014603944798950517	5.818304700777099E-6	0.0	7.563796111010228E-5	1.3382100811787327E-4
26	0.0015127592222020456	5.818304700777099E-6	0.0	7.563796111010228E-5	1.3963931281865037E-4
27	0.001716399886729244	5.818304700777099E-6	0.0	7.563796111010228E-5	1.3963931281865037E-4
28	0.0017978561525401236	5.818304700777099E-6	0.0	7.563796111010228E-5	1.4545761751942745E-4
29	0.001949132074760328	5.818304700777099E-6	0.0	7.563796111010228E-5	1.5709422692098166E-4
30	0.002071316473476647	5.818304700777099E-6	0.0	9.309287521243358E-5	1.6291253162175877E-4
31	0.0022749571380038455	5.818304700777099E-6	0.0	9.309287521243358E-5	1.6291253162175877E-4
32	0.002583327287145032	5.818304700777099E-6	0.0	9.309287521243358E-5	1.8036744572409006E-4
33	0.0028393326939792243	5.818304700777099E-6	0.0	9.309287521243358E-5	1.8036744572409006E-4
34	0.0030371550538056454	5.818304700777099E-6	0.0	9.309287521243358E-5	1.8618575042486716E-4
35	0.003234977413632067	5.818304700777099E-6	0.0	9.309287521243358E-5	1.9200405512564424E-4
36	0.003421163164056934	5.818304700777099E-6	0.0	9.891117991321067E-5	1.9782235982642135E-4
37	0.003944810587126873	5.818304700777099E-6	0.0	9.891117991321067E-5	2.0364066452719845E-4
38	0.004340455306779715	5.818304700777099E-6	0.0	9.891117991321067E-5	2.0364066452719845E-4
39	0.0045848241042123535	5.818304700777099E-6	0.0	9.891117991321067E-5	2.0364066452719845E-4
40	0.004858284425148877	5.818304700777099E-6	0.0	9.891117991321067E-5	2.0364066452719845E-4
41	0.005195746097793949	5.818304700777099E-6	0.0	9.891117991321067E-5	2.1527727392875264E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	156695	0.0	44.55094	1
TCAACGC	162980	0.0	43.137165	12
CAACGCA	163340	0.0	43.059353	13
AGTGGTA	161880	0.0	42.98462	5
GGTATCA	162705	0.0	42.960205	8
GTATCAA	162830	0.0	42.93444	9
ATCAACG	163705	0.0	42.933205	11
AGCAGTG	163420	0.0	42.891598	2
GTGGTAT	162255	0.0	42.879482	6
ACGCAGA	164275	0.0	42.78852	15
AACGCAG	164435	0.0	42.785473	14
TGGTATC	163005	0.0	42.73697	7
GCAGTGG	164655	0.0	42.63697	3
TATCAAC	164145	0.0	42.609093	10
CGCAGAG	165495	0.0	42.504333	16
AGAGTAC	162870	0.0	42.18659	19
CAGTGGT	165480	0.0	42.103455	4
CAGAGTA	166030	0.0	41.80404	18
GAGTACT	55350	0.0	41.62918	20
GCAGAGT	167570	0.0	41.567104	17
>>END_MODULE