FastQCFastQC Report
Thu 26 May 2016
SRR805392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20851197
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1038680.49813926749624976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT541650.039.1037561
CAACGCA615050.034.3148513
TCAACGC616500.034.24176812
AGTGGTA617700.034.0192645
GTGGTAT621700.033.7550286
GGTATCA623650.033.754998
TGGTATC624100.033.6591077
AACGCAG628400.033.6045514
GTATCAA626750.033.5655339
ACGCAGA629500.033.52716415
ATCAACG630400.033.50166311
CGCAGAG644050.032.7587916
TATCAAC648700.032.48774310
AGAGTAC640650.032.19901719
CAGTGGT660750.031.8774854
AGCAGTG671350.031.5386892
GCAGTGG672550.031.4439813
CAGAGTA667100.031.10200518
GCAGAGT683650.030.56563817
GAGTACT230950.029.86468920
Produced by FastQC (version 0.11.5)