##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805392_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20851197
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.796161294720875	37.0	35.0	37.0	35.0	37.0
2	36.015733245434305	37.0	35.0	37.0	35.0	37.0
3	37.868216678399804	39.0	38.0	39.0	35.0	39.0
4	37.87964690947958	39.0	38.0	39.0	35.0	39.0
5	37.8429309358115	39.0	38.0	39.0	35.0	39.0
6	37.70470644922687	39.0	38.0	39.0	35.0	39.0
7	37.733385809936955	39.0	38.0	39.0	35.0	39.0
8	38.72428959354228	40.0	39.0	40.0	36.0	40.0
9	38.667422210820796	40.0	39.0	40.0	36.0	40.0
10	38.58534399727747	40.0	39.0	40.0	36.0	40.0
11	38.63040697375791	40.0	39.0	40.0	36.0	40.0
12	38.662681763545756	40.0	39.0	40.0	36.0	40.0
13	38.706228280323664	40.0	39.0	40.0	36.0	40.0
14	38.68034861499798	40.0	39.0	40.0	36.0	40.0
15	38.667454870816286	40.0	39.0	40.0	36.0	40.0
16	38.62852310109583	40.0	39.0	40.0	36.0	40.0
17	38.579012274451195	40.0	39.0	40.0	36.0	40.0
18	38.542651196475674	40.0	39.0	40.0	35.0	40.0
19	38.511848504428784	40.0	39.0	40.0	35.0	40.0
20	38.360910455164756	40.0	39.0	40.0	35.0	40.0
21	38.34188785420808	40.0	38.0	40.0	35.0	40.0
22	38.29661313928404	40.0	38.0	40.0	35.0	40.0
23	38.193157400028404	40.0	38.0	40.0	34.0	40.0
24	38.04106670710559	40.0	38.0	40.0	34.0	40.0
25	38.120875554530514	40.0	38.0	40.0	34.0	40.0
26	38.16145269741588	40.0	38.0	40.0	34.0	40.0
27	38.16715275386828	40.0	38.0	40.0	34.0	40.0
28	38.0963924996728	40.0	38.0	40.0	34.0	40.0
29	38.05478836538737	40.0	38.0	40.0	34.0	40.0
30	38.023878053619654	40.0	38.0	40.0	34.0	40.0
31	38.07653411936015	40.0	38.0	40.0	34.0	40.0
32	38.007104771970646	40.0	38.0	40.0	34.0	40.0
33	38.04206012729149	40.0	38.0	40.0	34.0	40.0
34	38.0519669925904	40.0	38.0	40.0	34.0	40.0
35	38.030516473466726	40.0	38.0	40.0	34.0	40.0
36	38.0174324764185	40.0	38.0	40.0	34.0	40.0
37	37.93245505282023	40.0	38.0	40.0	34.0	40.0
38	37.86372609687588	40.0	38.0	40.0	33.0	40.0
39	37.87985581834942	40.0	38.0	40.0	33.0	40.0
40	37.80103137484146	40.0	38.0	40.0	33.0	40.0
41	37.74783788192112	40.0	38.0	40.0	33.0	40.0
42	37.703142989824514	40.0	38.0	40.0	33.0	40.0
43	37.6750041736213	40.0	38.0	40.0	33.0	40.0
44	37.594625526774315	40.0	37.0	40.0	33.0	40.0
45	37.503119940788054	40.0	37.0	40.0	33.0	40.0
46	37.44495320820191	40.0	37.0	40.0	33.0	40.0
47	37.328343835608095	40.0	37.0	40.0	33.0	40.0
48	37.188273172039	40.0	37.0	40.0	32.0	40.0
49	37.141797374990034	40.0	36.0	40.0	32.0	40.0
50	37.09865519950725	40.0	36.0	40.0	32.0	40.0
51	37.018178956344805	40.0	36.0	40.0	32.0	40.0
52	36.91366040040771	39.0	36.0	40.0	31.0	40.0
53	36.45446508418677	39.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	14.0
13	33.0
14	115.0
15	307.0
16	773.0
17	1862.0
18	3573.0
19	6434.0
20	10824.0
21	17248.0
22	25545.0
23	36720.0
24	50811.0
25	67885.0
26	88556.0
27	113786.0
28	144085.0
29	180650.0
30	223289.0
31	276719.0
32	341769.0
33	424371.0
34	543561.0
35	728466.0
36	1040908.0
37	1691096.0
38	3239191.0
39	1.1592599E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.168095097849775	38.22711473111112	14.60547804521726	18.99931212582184
2	29.899904547446365	29.068590162953235	25.98158753188126	15.049917757719136
3	24.423801664719775	25.24691987706989	25.98508373404174	24.344194724168595
4	22.203852373559176	30.599202530195267	23.74574946464704	23.451195631598512
5	22.732579813043827	30.359662325381127	22.983093009000875	23.92466485257417
6	24.182424634902254	25.416761445398073	28.210107074428386	22.190706845271283
7	21.938481517392024	26.644901968937322	27.05622607661325	24.360390437057404
8	23.4363763384903	25.39353016519867	27.211449779118198	23.95864371719283
9	24.64186108835862	25.940481978085	26.48221586511316	22.935441068443215
10	22.329245654338216	27.1410509430226	28.46384790283263	22.06585549980656
11	22.281166879771938	29.01073257329064	26.766386601210474	21.941713945726953
12	22.302211235163142	25.9562652446284	28.66946199779322	23.072061522415236
13	22.59097163582503	26.651031113465574	26.017575873461844	24.74042137724755
14	22.558431537527557	28.65353005872996	26.138748773032074	22.64928963071041
15	22.70267745300186	27.96632250896675	25.937983320573878	23.393016717457517
16	23.450202882836894	26.086646248654215	25.506746687012743	24.95640418149615
17	25.146666639809695	26.018041074572363	25.761911894074952	23.07338039154299
18	22.9136101874631	26.096779959443094	25.676842437391006	25.3127674157028
19	23.610126555324378	28.166833779374873	25.400843893998026	22.822195771302724
20	25.205176470204567	26.277834313301053	25.73657521915888	22.7804139973355
21	23.498085026005942	27.793905548923643	25.657802763073985	23.05020666199643
22	25.369003995310198	26.03554606481345	25.751509613572782	22.843940326303567
23	23.055055304498826	26.258156785914977	27.94754660847528	22.739241301110916
24	23.16584510711783	27.748493287939297	25.636734428244097	23.448927176698778
25	23.224805750960005	26.179720041971695	25.500895703973253	25.09457850309505
26	23.093360059856515	27.30043268019577	26.88183800671012	22.724369253237594
27	23.389611637164045	25.49447401029303	27.961977434676772	23.153936917866154
28	24.99036386256386	25.94099034218515	26.136293278510582	22.932352516740405
29	24.850165676339827	25.86436164791882	26.458279589416378	22.827193086324975
30	24.756147093138107	25.60337902903128	26.464974648697627	23.175499229132985
31	24.520170232912765	26.147966469263135	26.168166748412574	23.163696549411526
32	23.926578411781346	26.308302588096023	26.590013033784103	23.175105966338528
33	23.84336016776399	25.870673036181092	26.61030923068829	23.67565756536663
34	23.950457136825285	26.230503697221796	26.379502337443743	23.439536828509176
35	23.635098742772417	26.211128310763165	26.811631006124014	23.3421419403404
36	23.74094878102202	25.852597335299265	26.74846436873624	23.657989514942475
37	24.072896150758154	26.247874402606243	26.332190904915436	23.34703854172017
38	23.833111355669413	26.235822336722443	26.731760291747282	23.199306015860863
39	23.791953047108038	25.935259256339094	26.692189422026946	23.58059827452592
40	23.94310024503629	26.383382210623207	26.27512463672949	23.39839290761101
41	23.737778699227675	26.38359322968365	26.657246583973095	23.221381487115583
42	23.820114499901372	26.033397507107146	26.651472335137406	23.49501565785408
43	23.99738010244688	26.36511947011963	26.26384950465913	23.37365092277436
44	23.847005042444323	26.162920047227985	26.724245135662954	23.265829774664734
45	23.901102656120894	25.80193837313033	26.664469190905443	23.632489779843336
46	23.989318215160502	26.152532154389025	26.267820499705604	23.59032913074487
47	23.663821314431015	26.137477862781687	26.72686848625525	23.471832336532046
48	23.641438906361106	25.91726028966107	26.663759399520327	23.7775414044575
49	23.91731755256065	26.302087117588503	26.230120026202812	23.550475303648035
50	23.789013167925084	26.276769626223377	26.579668303934785	23.354548901916758
51	23.836142356719378	25.87105670720007	26.631737257098475	23.661063678982075
52	24.044696330862926	26.190362116860726	26.232268583909114	23.532672968367237
53	23.97942909464622	26.16543788829006	26.43626646470224	23.41886655236148
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5582.0
1	3669.5
2	1757.0
3	1523.0
4	1289.0
5	1346.0
6	1403.0
7	1589.0
8	2179.0
9	2583.0
10	3621.5
11	4660.0
12	6695.0
13	8730.0
14	11633.5
15	14537.0
16	20391.5
17	26246.0
18	36301.5
19	46357.0
20	62054.0
21	77751.0
22	163852.0
23	249953.0
24	222171.5
25	221769.5
26	249149.0
27	286361.0
28	323573.0
29	366948.0
30	410323.0
31	449891.0
32	489459.0
33	544706.0
34	599953.0
35	670406.0
36	740859.0
37	811814.0
38	882769.0
39	948319.5
40	1013870.0
41	1080759.0
42	1209901.0
43	1272154.0
44	1326905.5
45	1381657.0
46	1419916.0
47	1458175.0
48	1477110.5
49	1496046.0
50	1504176.0
51	1512306.0
52	1492598.5
53	1472891.0
54	1419734.5
55	1366578.0
56	1292138.5
57	1217699.0
58	1103490.5
59	868619.0
60	747956.0
61	646954.0
62	545952.0
63	460673.0
64	375394.0
65	304401.0
66	233408.0
67	186150.5
68	138893.0
69	107006.5
70	75120.0
71	56256.0
72	37392.0
73	28066.0
74	18740.0
75	14016.5
76	6784.0
77	4275.0
78	3066.5
79	1858.0
80	1385.0
81	912.0
82	636.5
83	361.0
84	243.5
85	126.0
86	90.0
87	54.0
88	40.0
89	26.0
90	23.0
91	20.0
92	13.0
93	4.5
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0851197E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.215572613520685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.16035426954932	36.73789082253451
2	14.395212958277884	14.457277231869941
3	4.915367124020757	7.404839242151301
4	2.2410706123244766	4.50146576260828
5	1.2885408278041393	3.235240775204241
6	0.7826808661681471	2.358166072096792
7	0.5450801587509682	1.9160058604364034
8	0.38645242165738153	1.5524743713125724
9	0.3074148538559306	1.38933116246496
>10	1.797033229032627	16.906412884992786
>50	0.1311721776354026	4.499529069477594
>100	0.04888092198369141	3.9268172314394265
>500	4.972544900901016E-4	0.15848975598038054
>1k	2.03596838429408E-4	0.2333497893583869
>5k	0.0	0.0
>10k+	3.8727610631625644E-5	0.7227099680723131
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	103868	0.49813926749624976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.153009153383376E-5	0.0	0.0	4.795887737284339E-6	1.9183550949137355E-5
2	9.112186700840245E-5	4.795887737284339E-6	0.0	4.795887737284339E-6	3.836710189827471E-5
3	9.112186700840245E-5	4.795887737284339E-6	0.0	9.591775474568678E-6	5.275476511012773E-5
4	9.112186700840245E-5	9.591775474568678E-6	0.0	1.4387663211853018E-5	5.755065284741207E-5
5	9.112186700840245E-5	1.4387663211853018E-5	0.0	1.4387663211853018E-5	7.673420379654942E-5
6	9.112186700840245E-5	2.3979438686421694E-5	0.0	1.4387663211853018E-5	8.63259792711181E-5
7	9.591775474568678E-5	2.3979438686421694E-5	0.0	1.4387663211853018E-5	8.63259792711181E-5
8	1.0071364248297112E-4	2.3979438686421694E-5	0.0	1.4387663211853018E-5	1.1510130569482414E-4
9	1.0071364248297112E-4	2.8775326423706036E-5	0.0	1.4387663211853018E-5	1.1989719343210847E-4
10	1.0550953022025546E-4	2.8775326423706036E-5	0.0	2.3979438686421694E-5	1.3908074438124583E-4
11	1.0550953022025546E-4	2.8775326423706036E-5	0.0	2.3979438686421694E-5	1.486725198558145E-4
12	1.103054179575398E-4	3.3571214160990375E-5	0.0	2.3979438686421694E-5	1.726519585422362E-4
13	1.2469308116939282E-4	3.3571214160990375E-5	0.0	2.3979438686421694E-5	2.0142728496594224E-4
14	1.3908074438124583E-4	3.3571214160990375E-5	0.0	2.3979438686421694E-5	2.1581494817779525E-4
15	1.582642953303832E-4	3.3571214160990375E-5	0.0	2.3979438686421694E-5	2.1581494817779525E-4
16	1.6306018306766753E-4	3.3571214160990375E-5	0.0	2.8775326423706036E-5	2.302026113896483E-4
17	1.726519585422362E-4	3.3571214160990375E-5	0.0	3.3571214160990375E-5	2.445902746015013E-4
18	2.206108359150796E-4	3.3571214160990375E-5	0.0	4.795887737284339E-5	2.589779378133543E-4
19	2.589779378133543E-4	3.3571214160990375E-5	0.0	5.275476511012773E-5	3.2612036613533505E-4
20	2.82957376499776E-4	3.3571214160990375E-5	0.0	5.275476511012773E-5	3.3571214160990376E-4
21	3.213244783980507E-4	3.3571214160990375E-5	0.0	6.234654058469641E-5	3.3571214160990376E-4
22	3.7407924350817845E-4	3.3571214160990375E-5	0.0	6.234654058469641E-5	3.405080293471881E-4
23	4.076504576691688E-4	3.3571214160990375E-5	0.0	6.234654058469641E-5	3.5969158029632546E-4
24	4.5081344730472787E-4	3.3571214160990375E-5	0.0	6.714242832198075E-5	3.692833557708941E-4
25	5.035682124148556E-4	3.3571214160990375E-5	0.0	6.714242832198075E-5	3.788751312454628E-4
26	5.94690079423258E-4	3.3571214160990375E-5	0.0	7.193831605926509E-5	3.932627944573158E-4
27	6.954037219062292E-4	3.3571214160990375E-5	0.0	7.673420379654942E-5	4.2203812088102184E-4
28	7.529543747536412E-4	3.3571214160990375E-5	0.0	8.63259792711181E-5	4.316298963555905E-4
29	8.584639049738967E-4	3.836710189827471E-5	0.0	8.63259792711181E-5	4.412216718301592E-4
30	9.927487616178583E-4	3.836710189827471E-5	0.0	8.63259792711181E-5	4.412216718301592E-4
31	0.001131829505999104	3.836710189827471E-5	0.0	8.63259792711181E-5	4.412216718301592E-4
32	0.0013236650154904775	3.836710189827471E-5	0.0	9.112186700840245E-5	4.795887737284339E-4
33	0.0015394799636682728	3.836710189827471E-5	0.0	9.591775474568678E-5	5.179558756267086E-4
34	0.0018176414524307646	3.836710189827471E-5	0.0	9.591775474568678E-5	5.179558756267086E-4
35	0.002095802941193256	3.836710189827471E-5	0.0	1.0550953022025546E-4	5.275476511012773E-4
36	0.0022828425629473456	3.836710189827471E-5	0.0	1.1989719343210847E-4	5.611188652622676E-4
37	0.0025514122762352685	3.836710189827471E-5	0.0	1.2948896890667714E-4	5.707106407368364E-4
38	0.0028343696527350446	3.836710189827471E-5	0.0	1.3908074438124583E-4	5.755065284741207E-4
39	0.0031221229169721048	4.316298963555905E-5	0.0	1.3908074438124583E-4	6.282612935842485E-4
40	0.003496202160480283	4.316298963555905E-5	0.0	1.4387663211853018E-4	6.426489567961014E-4
41	0.0038127307511410494	4.316298963555905E-5	0.0	1.4387663211853018E-4	6.666283954825231E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	54165	0.0	39.103756	1
CAACGCA	61505	0.0	34.31485	13
TCAACGC	61650	0.0	34.241768	12
AGTGGTA	61770	0.0	34.019264	5
GTGGTAT	62170	0.0	33.755028	6
GGTATCA	62365	0.0	33.75499	8
TGGTATC	62410	0.0	33.659107	7
AACGCAG	62840	0.0	33.60455	14
GTATCAA	62675	0.0	33.565533	9
ACGCAGA	62950	0.0	33.527164	15
ATCAACG	63040	0.0	33.501663	11
CGCAGAG	64405	0.0	32.75879	16
TATCAAC	64870	0.0	32.487743	10
AGAGTAC	64065	0.0	32.199017	19
CAGTGGT	66075	0.0	31.877485	4
AGCAGTG	67135	0.0	31.538689	2
GCAGTGG	67255	0.0	31.443981	3
CAGAGTA	66710	0.0	31.102005	18
GCAGAGT	68365	0.0	30.565638	17
GAGTACT	23095	0.0	29.864689	20
>>END_MODULE