FastQCFastQC Report
Thu 26 May 2016
SRR805391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20562019
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4695852.2837494703219563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG819490.3985454930277032No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG476700.23183521034583227No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC308210.14989286801067542No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA211780.10299572235586399No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2290500.045.2301861
TCAACGC2352400.044.08197812
CAACGCA2355000.044.0233313
GTGGTAT2340650.043.9428376
GTATCAA2353150.043.8861739
AGTGGTA2346000.043.862665
ATCAACG2365200.043.85434311
GGTATCA2356050.043.826178
AACGCAG2366550.043.81741714
ACGCAGA2364650.043.807915
TGGTATC2351550.043.768137
TATCAAC2367400.043.64285710
AGCAGTG2376400.043.6071172
CGCAGAG2384050.043.44254316
GCAGTGG2387550.043.334573
AGAGTAC2339300.043.29814519
CAGTGGT2394850.042.995434
CAGAGTA2369000.042.91502818
GTACTTT758550.042.71854422
GCAGAGT2400050.042.6888217