##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805391_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20562019
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.940173919691446	37.0	35.0	37.0	35.0	37.0
2	36.10752387691112	37.0	35.0	37.0	35.0	37.0
3	37.96976099477391	39.0	38.0	39.0	35.0	39.0
4	37.931438979800575	39.0	38.0	39.0	35.0	39.0
5	37.85132554346925	39.0	38.0	39.0	35.0	39.0
6	37.77265243262347	39.0	38.0	39.0	35.0	39.0
7	37.64630535551981	39.0	37.0	39.0	35.0	39.0
8	38.70063217041089	40.0	39.0	40.0	36.0	40.0
9	38.697400775672854	40.0	39.0	40.0	36.0	40.0
10	38.32478746372134	40.0	38.0	40.0	34.0	40.0
11	38.55729245265263	40.0	38.0	40.0	36.0	40.0
12	38.69459521460417	40.0	39.0	40.0	36.0	40.0
13	38.77203157919463	40.0	39.0	40.0	36.0	40.0
14	38.76364154706792	40.0	39.0	40.0	36.0	40.0
15	38.736005934047625	40.0	39.0	40.0	36.0	40.0
16	38.68090220128675	40.0	39.0	40.0	36.0	40.0
17	38.631474321660725	40.0	39.0	40.0	36.0	40.0
18	38.60486516426232	40.0	39.0	40.0	36.0	40.0
19	38.55948873503132	40.0	39.0	40.0	36.0	40.0
20	38.44580189328684	40.0	39.0	40.0	35.0	40.0
21	38.35668997290587	40.0	38.0	40.0	35.0	40.0
22	38.388347078173595	40.0	39.0	40.0	35.0	40.0
23	37.87189837729456	40.0	38.0	40.0	34.0	40.0
24	38.00018636302204	40.0	38.0	40.0	34.0	40.0
25	38.15965562525742	40.0	38.0	40.0	34.0	40.0
26	38.19224649096959	40.0	38.0	40.0	34.0	40.0
27	38.2337567142604	40.0	38.0	40.0	35.0	40.0
28	38.1821112022122	40.0	38.0	40.0	34.0	40.0
29	38.124548664214345	40.0	38.0	40.0	34.0	40.0
30	38.08454777714192	40.0	38.0	40.0	34.0	40.0
31	38.102366990323276	40.0	38.0	40.0	34.0	40.0
32	38.02702959276519	40.0	38.0	40.0	34.0	40.0
33	38.0006288292993	40.0	38.0	40.0	34.0	40.0
34	38.006378556502646	40.0	38.0	40.0	34.0	40.0
35	37.987876044662734	40.0	38.0	40.0	34.0	40.0
36	37.9591659748977	40.0	38.0	40.0	34.0	40.0
37	37.83013146714824	40.0	38.0	40.0	33.0	40.0
38	37.7008592395523	40.0	37.0	40.0	33.0	40.0
39	37.721746098960416	40.0	37.0	40.0	33.0	40.0
40	37.65275700795724	40.0	37.0	40.0	33.0	40.0
41	37.58232053963183	40.0	37.0	40.0	33.0	40.0
42	37.53406793369854	40.0	37.0	40.0	33.0	40.0
43	37.4948672112403	40.0	37.0	40.0	33.0	40.0
44	37.412012069437345	40.0	37.0	40.0	33.0	40.0
45	37.31619035076273	40.0	36.0	40.0	33.0	40.0
46	37.244690854531356	40.0	36.0	40.0	33.0	40.0
47	37.116876460429296	40.0	36.0	40.0	32.0	40.0
48	36.95408933334805	40.0	36.0	40.0	32.0	40.0
49	36.87056139769154	39.0	35.0	40.0	32.0	40.0
50	36.78580367034969	39.0	35.0	40.0	31.0	40.0
51	36.64012391001098	39.0	35.0	40.0	31.0	40.0
52	36.46232244995007	39.0	35.0	40.0	31.0	40.0
53	35.91598577941203	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	21.0
14	87.0
15	214.0
16	621.0
17	1421.0
18	2885.0
19	5327.0
20	9169.0
21	14907.0
22	23070.0
23	33140.0
24	47662.0
25	65524.0
26	86731.0
27	113035.0
28	143936.0
29	180519.0
30	223804.0
31	275156.0
32	341347.0
33	428948.0
34	556753.0
35	766922.0
36	1134513.0
37	1989445.0
38	3255457.0
39	1.0861402E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.015302631516876	36.514668136431546	29.401713907569093	21.068315324482484
2	27.187860297181903	42.36375328706777	12.203986388690721	18.244400027059598
3	36.164400976382716	25.206999371024803	17.402230782881777	21.2263688697107
4	22.360746772970106	22.796170940217493	19.362797009379285	35.48028527743312
5	20.809206527822	33.902716459896276	22.938608314679605	22.349468697602116
6	32.84837447139797	22.91909661205935	21.24741738639576	22.985111530146916
7	22.650100654026243	22.164564676260635	33.08725179176228	22.098082877950848
8	32.2597017345427	24.2873425999655	22.303296188958875	21.14965947653292
9	32.24177547934374	23.68761550118206	22.65796466776925	21.412644351704955
10	21.30120101532831	23.96495694318734	33.28617680977729	21.44766523170706
11	21.19953298360438	34.46723787192298	22.883569945149844	21.44965919932279
12	20.988984593390366	23.682085888550148	33.6252339811572	21.703695536902284
13	21.05638556213765	23.819791237426635	23.07301632198667	32.050806878449045
14	21.585176047157624	34.556708657841426	22.68138162891494	21.176733666086
15	21.278367654460393	34.18558751453347	22.764004838240837	21.7720399927653
16	21.518237095296914	23.35001733049658	23.016732938530986	32.11501263567552
17	32.22029412578599	23.306913586647305	22.922977553906552	21.549814733660153
18	21.35267942316365	23.552550943562498	22.919830975742215	32.17493865753164
19	21.6518280622151	34.03725577726584	22.988496411758007	21.322419748761053
20	32.357313744336096	23.510327463465526	22.96941268267479	21.162946109523585
21	21.59654652590293	34.01651851406226	22.983774112843687	21.403160847191124
22	31.980614355039744	23.180831609969818	23.372758287987185	21.46579574700325
23	21.56970577646096	23.55588232848146	33.48581673813257	21.388595156925007
24	21.643049741370245	33.71589628430943	22.951539924167953	21.68951405015237
25	21.389144713853245	23.437499012134946	23.150615705588056	32.02274056842375
26	21.752460203445974	30.481038851291792	26.449922062614572	21.31657888264766
27	21.91338798004223	23.22175657944874	33.47541406318125	21.38944137732778
28	28.943500149474623	23.18359398461795	26.457260836107583	21.415645029799848
29	29.01321120265476	23.358620571258104	26.23232183571078	21.395846390376352
30	28.849433511368705	23.313036526228284	26.276335023326258	21.56119493907675
31	26.224384871933054	24.282761337784972	27.10220236641159	22.390651423870388
32	24.903862796741898	25.338202440139757	27.259122754433797	22.498812008684553
33	24.539910210179265	24.748970419684955	27.660430622109626	23.050688748026154
34	23.683992316124208	24.602506203306202	28.093282084799164	23.620219395770427
35	23.458917142329263	24.781759028624574	28.359398948128582	23.39992488091758
36	23.71114431904766	24.856814887681995	28.2647389830736	23.167301810196754
37	24.18048538910503	24.64017760123653	28.210016730360966	22.969320279297474
38	24.149024470797347	24.80891103154802	28.15588294126175	22.88618155639288
39	24.166080188915302	24.774979538731095	28.10319356285003	22.955746709503575
40	23.583501211627127	25.304518977440882	27.869962575173187	23.2420172357588
41	24.058517794385853	25.47315027770376	27.60049487358221	22.867837054328177
42	23.85835748911622	25.528125423870097	27.75671494127109	22.856802145742595
43	23.615326880108416	25.083193435430633	28.532630963914585	22.768848720546362
44	24.11403763414478	24.82344754180025	27.928872159878853	23.133642664176122
45	24.236797952574598	24.47657012669816	27.914646903108103	23.371985017619135
46	23.76046826919088	24.568973504012423	27.776421177317268	23.89413704947943
47	23.670749453154382	24.796825642462444	27.965964820867057	23.566460083516116
48	23.478769278444883	24.804203322640642	27.875540821161582	23.841486577752896
49	23.243014219566668	25.425154018192476	27.781406096356587	23.55042566588427
50	24.15175766543159	25.149587693698756	27.481070803406997	23.217583837462655
51	23.872845366011965	24.877469474179552	27.878774939367577	23.3709102204409
52	24.201178882287774	24.8098691086707	27.631445141646836	23.357506867394683
53	24.701844697254682	24.966745726672073	26.996833336259442	23.334576239813803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3516.0
1	2098.0
2	680.0
3	602.5
4	525.0
5	580.5
6	636.0
7	725.0
8	1068.5
9	1323.0
10	1962.5
11	2602.0
12	4024.0
13	5446.0
14	7061.0
15	8676.0
16	12875.5
17	17075.0
18	23636.5
19	30198.0
20	45008.5
21	59819.0
22	338824.5
23	617830.0
24	418228.0
25	220671.5
26	222717.0
27	243079.5
28	263442.0
29	291339.5
30	319237.0
31	346038.0
32	372839.0
33	418769.5
34	464700.0
35	523565.5
36	582431.0
37	648551.0
38	714671.0
39	780511.0
40	846351.0
41	915975.0
42	1056199.5
43	1126800.0
44	1200832.0
45	1274864.0
46	1329719.0
47	1384574.0
48	1446771.0
49	1508968.0
50	1521628.5
51	1534289.0
52	1526015.5
53	1517742.0
54	1486850.5
55	1455959.0
56	1442158.5
57	1428358.0
58	1269259.5
59	969905.5
60	829650.0
61	722000.5
62	614351.0
63	521072.5
64	427794.0
65	348405.5
66	269017.0
67	215562.0
68	162107.0
69	126409.0
70	90711.0
71	67520.5
72	44330.0
73	32986.0
74	21642.0
75	16329.0
76	8079.5
77	5143.0
78	3755.0
79	2367.0
80	1862.0
81	1357.0
82	1015.5
83	674.0
84	449.5
85	225.0
86	156.0
87	87.0
88	60.5
89	34.0
90	25.0
91	16.0
92	17.0
93	13.5
94	9.0
95	5.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0562019E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.83244379699349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.60047045531792	34.000574522339626
2	14.563764159268636	13.64113332923233
3	4.862876010717971	6.8322110239109195
4	2.296898042803995	4.302773939881697
5	1.285876795820893	3.0110376385070023
6	0.79526797747841	2.2346605715283787
7	0.5618172222367079	1.841789143920854
8	0.4088555582924656	1.5318163963856233
9	0.3174975334359311	1.3382266851290074
>10	2.1208729351226823	17.892758973808522
>50	0.13021957958602567	4.179876096114048
>100	0.0531411666633809	4.241923079366894
>500	0.0014633138759668944	0.4571215771184675
>1k	8.303276480700679E-4	0.7952007745127881
>5k	6.382359915838488E-5	0.2090766259875902
>10k+	8.509813221117971E-5	3.4898196222559594
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	469585	2.2837494703219563	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	81949	0.3985454930277032	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	47670	0.23183521034583227	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	30821	0.14989286801067542	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	21178	0.10299572235586399	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.653534120360457E-4	0.0	0.0	0.0	9.726671296237981E-6
2	1.7994341898040265E-4	0.0	0.0	4.8633356481189906E-6	1.4590006944356973E-5
3	1.8967009027664065E-4	4.8633356481189906E-6	0.0	4.8633356481189906E-6	2.9180013888713945E-5
4	2.091234328691166E-4	1.4590006944356973E-5	0.0	4.8633356481189906E-6	3.4043349536832935E-5
5	2.1398676851723558E-4	1.4590006944356973E-5	0.0	4.8633356481189906E-6	4.8633356481189904E-5
6	2.1885010416535457E-4	1.4590006944356973E-5	0.0	4.8633356481189906E-6	5.3496692129308894E-5
7	2.2371343981347357E-4	1.4590006944356973E-5	0.0	4.8633356481189906E-6	5.3496692129308894E-5
8	2.3344011110971156E-4	1.4590006944356973E-5	0.0	4.8633356481189906E-6	5.836002777742789E-5
9	2.3344011110971156E-4	1.4590006944356973E-5	0.0	4.8633356481189906E-6	6.808669907366587E-5
10	2.3830344675783053E-4	1.4590006944356973E-5	0.0	4.8633356481189906E-6	7.295003472178487E-5
11	2.480301180540685E-4	1.4590006944356973E-5	0.0	1.4590006944356973E-5	8.754004166614183E-5
12	2.626201249984255E-4	1.9453342592475962E-5	0.0	1.4590006944356973E-5	1.021300486104988E-4
13	2.8207346759090147E-4	2.4316678240594952E-5	0.0	1.4590006944356973E-5	1.021300486104988E-4
14	2.9180013888713946E-4	2.4316678240594952E-5	0.0	1.4590006944356973E-5	1.1185671990673678E-4
15	3.112534814796154E-4	2.9180013888713945E-5	0.0	1.4590006944356973E-5	1.1185671990673678E-4
16	3.209801527758534E-4	3.8906685184951925E-5	0.0	1.4590006944356973E-5	1.1672005555485578E-4
17	3.501601666645673E-4	3.8906685184951925E-5	0.0	1.4590006944356973E-5	1.3131006249921274E-4
18	3.696135092570433E-4	4.8633356481189904E-5	0.0	1.4590006944356973E-5	1.3617339814733174E-4
19	3.9393018749763826E-4	5.3496692129308894E-5	0.0	1.4590006944356973E-5	1.3617339814733174E-4
20	4.3770020833070914E-4	5.3496692129308894E-5	0.0	1.4590006944356973E-5	1.507634050916887E-4
21	4.96060236108137E-4	5.3496692129308894E-5	0.0	1.4590006944356973E-5	1.604900763879267E-4
22	5.44693592589327E-4	5.3496692129308894E-5	0.0	1.4590006944356973E-5	1.604900763879267E-4
23	5.884636134223978E-4	5.836002777742789E-5	0.0	1.9453342592475962E-5	1.7021674768416466E-4
24	6.419603055517068E-4	5.836002777742789E-5	0.0	1.9453342592475962E-5	1.7994341898040265E-4
25	7.003203333291346E-4	5.836002777742789E-5	0.0	2.4316678240594952E-5	1.7994341898040265E-4
26	7.684070324028005E-4	5.836002777742789E-5	0.0	2.4316678240594952E-5	1.8967009027664065E-4
27	8.316303958283474E-4	7.781337036990385E-5	0.0	2.4316678240594952E-5	2.042600972209976E-4
28	9.288971087907272E-4	7.781337036990385E-5	0.0	2.4316678240594952E-5	2.1885010416535457E-4
29	9.726671296237981E-4	7.781337036990385E-5	0.0	2.4316678240594952E-5	2.1885010416535457E-4
30	0.001084523849530535	7.781337036990385E-5	0.0	2.4316678240594952E-5	2.2857677546159256E-4
31	0.0012304239189741047	7.781337036990385E-5	0.0	2.4316678240594952E-5	2.3344011110971156E-4
32	0.0014249573448988642	7.781337036990385E-5	0.0	2.4316678240594952E-5	2.3830344675783053E-4
33	0.001565994078694315	7.781337036990385E-5	0.0	2.4316678240594952E-5	2.3830344675783053E-4
34	0.0017070308124897658	8.267670601802285E-5	0.0	2.9180013888713945E-5	2.4316678240594953E-4
35	0.002130141013876118	8.267670601802285E-5	0.0	3.4043349536832935E-5	2.4316678240594953E-4
36	0.0023733077962820675	8.267670601802285E-5	0.0	3.8906685184951925E-5	2.480301180540685E-4
37	0.002616474578688017	8.267670601802285E-5	0.0	3.8906685184951925E-5	2.480301180540685E-4
38	0.0029471814027601084	8.267670601802285E-5	0.0	4.8633356481189904E-5	2.480301180540685E-4
39	0.003224391534702891	8.754004166614183E-5	0.0	4.8633356481189904E-5	2.577567893503065E-4
40	0.00351619167359003	8.754004166614183E-5	0.0	4.8633356481189904E-5	2.577567893503065E-4
41	0.0038079918124771695	9.240337731426083E-5	0.0	4.8633356481189904E-5	2.7721013194278245E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	229050	0.0	45.230186	1
TCAACGC	235240	0.0	44.081978	12
CAACGCA	235500	0.0	44.02333	13
GTGGTAT	234065	0.0	43.942837	6
GTATCAA	235315	0.0	43.886173	9
AGTGGTA	234600	0.0	43.86266	5
ATCAACG	236520	0.0	43.854343	11
GGTATCA	235605	0.0	43.82617	8
AACGCAG	236655	0.0	43.817417	14
ACGCAGA	236465	0.0	43.8079	15
TGGTATC	235155	0.0	43.76813	7
TATCAAC	236740	0.0	43.642857	10
AGCAGTG	237640	0.0	43.607117	2
CGCAGAG	238405	0.0	43.442543	16
GCAGTGG	238755	0.0	43.33457	3
AGAGTAC	233930	0.0	43.298145	19
CAGTGGT	239485	0.0	42.99543	4
CAGAGTA	236900	0.0	42.915028	18
GTACTTT	75855	0.0	42.718544	22
GCAGAGT	240005	0.0	42.68882	17
>>END_MODULE