FastQCFastQC Report
Thu 26 May 2016
SRR805390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21006320
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT305610.1454847874354004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT233250.028.985851
TCAACGC330600.020.43632912
CAACGCA331600.020.39595813
AGCAGTG343150.019.819032
ACGCAGA341150.019.81122815
AGTGGTA341450.019.786945
AACGCAG342150.019.7739314
GGTATCA346600.019.4929318
GTATCAA347300.019.440119
ATCAACG349050.019.37630811
GTGGTAT349700.019.3201316
TGGTATC354350.019.0533377
TATCAAC360300.018.77782610
CGCAGAG360650.018.77263316
GCAGTGG372800.018.2238463
AGAGTAC366700.018.20657219
CAGTGGT389550.017.434224
CAGAGTA390500.017.16312418
GCAGAGT410500.016.45286217
GTACATG282850.016.22609322
Produced by FastQC (version 0.11.5)