FastQCFastQC Report
Thu 26 May 2016
SRR805389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18211796
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8031224.409900045003798No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG326310.17917507971207233No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA303190.16648001108731944No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG287550.15789217054704544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC272950.14987538845701984No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG272410.14957887733862163No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC231880.12732407061884504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3144600.045.828961
AGCAGTG3250300.044.38272
GCAGTGG3278350.044.0008053
AGTGGTA3293500.043.5722165
GAGTACT1559500.043.44975720
GTGGTAT3296650.043.429366
TGGTATC3305100.043.3026857
TCAACGC3324850.043.29990812
CAACGCA3333100.043.2921413
GGTATCA3316450.043.2756548
GTATCAA3320250.043.2678839
ATCAACG3333100.043.21599611
AACGCAG3336350.043.2091214
CAGTGGT3322150.043.2035264
ACGCAGA3335200.043.19724315
CGCAGAG3335800.043.11197716
GTACTTT1566650.042.97545622
TATCAAC3345450.042.95390310
AGTACTT1572600.042.9309121
AGAGTAC3284250.042.8684919