##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805389_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18211796
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8330348637773	35.0	32.0	37.0	28.0	37.0
2	35.016560365600405	35.0	35.0	37.0	33.0	37.0
3	37.61031295320901	39.0	37.0	39.0	35.0	39.0
4	37.806372529101466	39.0	37.0	39.0	35.0	39.0
5	37.76979338007081	39.0	37.0	39.0	35.0	39.0
6	37.865782649882526	39.0	38.0	39.0	35.0	39.0
7	37.65002770731673	39.0	37.0	39.0	35.0	39.0
8	38.79945047704246	40.0	39.0	40.0	36.0	40.0
9	38.861395767885824	40.0	39.0	40.0	36.0	40.0
10	38.23124605612758	40.0	38.0	40.0	34.0	40.0
11	38.59470894578437	40.0	38.0	40.0	36.0	40.0
12	38.70908294821664	40.0	39.0	40.0	36.0	40.0
13	38.86618513627102	40.0	39.0	40.0	36.0	40.0
14	38.84476429452647	40.0	39.0	40.0	36.0	40.0
15	38.887827263165036	40.0	39.0	40.0	36.0	40.0
16	38.825790877516965	40.0	39.0	40.0	36.0	40.0
17	38.634794723156354	40.0	39.0	40.0	36.0	40.0
18	38.643227719001466	40.0	39.0	40.0	36.0	40.0
19	38.63154408274725	40.0	39.0	40.0	36.0	40.0
20	38.52816734823957	40.0	39.0	40.0	36.0	40.0
21	38.35375220543872	40.0	38.0	40.0	35.0	40.0
22	38.41800649425241	40.0	39.0	40.0	35.0	40.0
23	37.700922138596326	40.0	37.0	40.0	34.0	40.0
24	37.652002251727396	40.0	38.0	40.0	34.0	40.0
25	38.06292158115542	40.0	38.0	40.0	34.0	40.0
26	38.15485600651358	40.0	38.0	40.0	34.0	40.0
27	38.18420610465876	40.0	38.0	40.0	35.0	40.0
28	38.21192539165275	40.0	38.0	40.0	35.0	40.0
29	38.1858090767105	40.0	38.0	40.0	35.0	40.0
30	38.06239357172681	40.0	38.0	40.0	34.0	40.0
31	37.83037395103701	40.0	38.0	40.0	33.0	40.0
32	37.81780638219317	40.0	38.0	40.0	33.0	40.0
33	37.76077686132658	40.0	38.0	40.0	33.0	40.0
34	37.72560092370901	40.0	38.0	40.0	33.0	40.0
35	37.61415853768624	40.0	38.0	40.0	33.0	40.0
36	37.47815339025322	40.0	37.0	40.0	33.0	40.0
37	37.231495949109025	40.0	36.0	40.0	33.0	40.0
38	37.01869678311793	40.0	35.0	40.0	32.0	40.0
39	30.306760080115108	38.0	29.0	40.0	3.0	40.0
40	32.279457171604605	38.0	29.0	40.0	15.0	40.0
41	34.61403361865024	38.0	30.0	40.0	25.0	40.0
42	35.81115272760578	38.0	34.0	40.0	30.0	40.0
43	36.22355334970807	38.0	35.0	40.0	30.0	40.0
44	36.32330666343945	38.0	35.0	40.0	31.0	40.0
45	36.3451967614836	38.0	35.0	40.0	31.0	40.0
46	36.12123532462147	38.0	35.0	40.0	30.0	40.0
47	35.992281650859695	38.0	35.0	40.0	30.0	40.0
48	35.79686297825871	38.0	35.0	40.0	29.0	40.0
49	35.963724555227834	38.0	35.0	40.0	30.0	40.0
50	35.920207704940246	38.0	35.0	40.0	30.0	40.0
51	35.759351631217484	38.0	35.0	40.0	29.0	40.0
52	35.45724144944299	38.0	34.0	40.0	28.0	40.0
53	35.05169391310994	38.0	34.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	8.0
13	33.0
14	76.0
15	264.0
16	637.0
17	1357.0
18	2665.0
19	4866.0
20	8608.0
21	13745.0
22	21285.0
23	31368.0
24	45332.0
25	62729.0
26	83194.0
27	108784.0
28	140004.0
29	178902.0
30	226915.0
31	289240.0
32	376526.0
33	507796.0
34	726087.0
35	1100184.0
36	1788334.0
37	3145832.0
38	3971536.0
39	5375487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.709657850329535	42.04269584394642	25.65495462391518	20.592691681808866
2	21.884151348938897	43.47229674657019	17.99653367520699	16.647018229283923
3	37.561957096378634	23.60268586360181	19.685246858684337	19.15011018133522
4	18.820197634544115	22.160757785777964	21.64901803204912	37.3700265476288
5	19.26547497017867	39.234290786037796	23.034932963228886	18.465301280554648
6	36.4161118431153	22.425377486108456	21.745202944289517	19.41330772648672
7	20.13547702818547	22.045338087468146	39.35003994114584	18.46914494320055
8	35.790072544190586	23.225490775319468	22.46287516069255	18.521561519797388
9	35.37837234724132	22.670647090490142	23.459476484362114	18.491504077906427
10	18.58009501094785	23.231333142541242	39.32035039267956	18.868221453831353
11	18.416041998274086	39.837334000446745	22.934712205210296	18.811911796068877
12	18.409513262722687	23.169109735250714	39.2730953059215	19.148281696105094
13	18.362104429458796	23.378358729693655	22.545025213328767	35.71451162751878
14	18.97543767786549	40.037286822233234	22.15862180753617	18.8286536923651
15	18.807370783200074	39.617119585569704	22.141918347866405	19.433591283363818
16	19.176944437550254	22.819413307726485	22.29827305335509	35.70536920136817
17	36.2242142400453	22.569355597877333	22.254900065869396	18.951530096207975
18	18.988204128796525	23.109055251881802	22.314004615470104	35.588736003851565
19	19.469106726211958	39.5764755985626	22.24456061335192	18.709857061873524
20	36.12858391341524	22.947764185366452	22.391871729729456	18.53178017148885
21	19.256574145680087	39.56078247307405	22.472325079854837	18.710318301391034
22	35.7005810959007	22.509251695988688	22.851892257084362	18.938274951026244
23	18.99690178826954	23.059636732148768	39.1478413221848	18.795620157396886
24	18.970896664996687	38.96593724199415	22.821302193369615	19.24186389963955
25	18.881926856637314	22.76983005959434	22.877622833025367	35.47062025074298
26	19.203866548911485	31.050962793565223	31.064393649039335	18.68077700848395
27	19.67054759453708	22.389093310731134	39.25752298125896	18.682836113472828
28	27.96859793509657	22.405796770400897	30.82365407563318	18.801951218869352
29	28.01318991273568	22.543355965551118	30.68121342892266	18.76224069279054
30	27.75797071304774	22.506797242841948	30.70431933237117	19.03091271173914
31	24.30027219720669	23.65460825500132	31.6781826460169	20.36693690177509
32	22.762658883286417	25.227550319584076	32.09105790554649	19.918732891583016
33	22.317409002385048	24.66515109218223	32.31041573274816	20.70702417268456
34	21.54281763314283	24.434712534667092	32.83906211117234	21.18340772101774
35	21.117258286881754	24.642462500678132	32.97549566226198	21.264783550178137
36	21.180426136993844	24.862561605675793	33.00616809017628	20.950844167154077
37	21.78350778802925	24.608918307672674	32.902059741938686	20.705514162359385
38	21.852172075724987	24.685308357286672	32.87286437866974	20.589655188318602
39	18.88824144526987	37.772029732817124	26.758261513581637	16.58146730833137
40	21.3637084447904	25.011102694100025	32.65253465391332	20.97265420719626
41	21.549533060879885	25.340910912905017	32.46478271555425	20.64477331066085
42	21.573242968458466	25.195796175182284	32.6378408807127	20.593119975646555
43	21.43255942467179	24.80894800271209	33.136056432874604	20.622436139741517
44	21.481538668673863	24.68879510840117	32.89133592315662	20.93833029976835
45	21.6142328851037	24.45542987632851	32.82727853968933	21.103058698878463
46	21.69423048665821	24.541747557462205	32.50984691460414	21.254175041275445
47	21.45190952062059	24.79435855749757	32.472107638368016	21.28162428351383
48	21.26651868931543	24.850190502902624	32.48380884565147	21.39948196213048
49	20.978068280580345	25.304170988956827	32.39728250854556	21.320478221917266
50	21.739080538789253	25.166891832085092	32.08633569143867	21.00769193768698
51	21.694246959498116	25.098551510240945	32.033441402484414	21.173760127776525
52	21.987458018967487	25.125352820776158	31.59021218994546	21.29697697031089
53	22.55742926178176	25.399631096241137	30.743557637039203	21.299382004937897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15330.0
1	9337.0
2	3344.0
3	2637.5
4	1931.0
5	1946.0
6	1961.0
7	2388.0
8	3310.0
9	3805.0
10	5230.5
11	6656.0
12	9432.5
13	12209.0
14	15619.5
15	19030.0
16	26710.0
17	34390.0
18	46435.5
19	58481.0
20	85512.5
21	112544.0
22	691130.0
23	1269716.0
24	834103.5
25	378439.5
26	358388.0
27	372671.5
28	386955.0
29	407878.5
30	428802.0
31	452161.0
32	475520.0
33	513795.5
34	552071.0
35	601255.0
36	650439.0
37	701785.5
38	753132.0
39	803520.5
40	853909.0
41	897418.5
42	984407.5
43	1027887.0
44	1066802.0
45	1105717.0
46	1131105.5
47	1156494.0
48	1175137.0
49	1193780.0
50	1177687.0
51	1161594.0
52	1124711.0
53	1087828.0
54	1045746.0
55	1003664.0
56	960663.0
57	917662.0
58	805993.0
59	600305.0
60	506286.0
61	442365.5
62	378445.0
63	322256.0
64	266067.0
65	211882.5
66	157698.0
67	128213.0
68	98728.0
69	77328.0
70	55928.0
71	42135.5
72	28343.0
73	20711.0
74	13079.0
75	9847.0
76	5191.5
77	3768.0
78	3196.5
79	2625.0
80	2320.5
81	2016.0
82	1701.5
83	1387.0
84	958.0
85	529.0
86	362.5
87	196.0
88	169.5
89	143.0
90	113.0
91	83.0
92	61.0
93	26.0
94	13.0
95	10.0
96	7.0
97	5.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8211796E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.90873535463232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.7589961846755	30.429708275531326
2	18.336516953069765	16.46939574342278
3	6.452358941043143	8.693018402892063
4	2.6887036375546125	4.829851204239094
5	1.243237043058765	2.7916101674900844
6	0.7129846705988669	1.9211543930300525
7	0.4315320906232736	1.3565692318382263
8	0.29936517674960916	1.075528919763274
9	0.2499148114404087	1.0101022315362136
>10	1.6277973057695387	14.726462143214325
>50	0.130347285577029	4.0341858304344855
>100	0.06493475709286707	5.05387801686787
>500	0.002179407707893243	0.6444861553041317
>1k	9.05431491174594E-4	0.8067412049946001
>5k	6.286209656746717E-5	0.20770361474318602
>10k+	1.634414510754146E-4	5.949604464698447
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	803122	4.409900045003798	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	32631	0.17917507971207233	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	30319	0.16648001108731944	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	28755	0.15789217054704544	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	27295	0.14987538845701984	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	27241	0.14957887733862163	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	23188	0.12732407061884504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7021934574711908E-4	0.0	0.0	0.0	0.0
2	2.2512881211715746E-4	0.0	0.0	0.0	0.0
3	2.2512881211715746E-4	0.0	0.0	0.0	0.0
4	2.306197587541613E-4	0.0	0.0	0.0	0.0
5	2.4160165202816899E-4	0.0	0.0	0.0	0.0
6	2.690563852131882E-4	0.0	0.0	0.0	0.0
7	2.690563852131882E-4	0.0	0.0	0.0	0.0
8	2.690563852131882E-4	0.0	0.0	5.490946637003841E-6	0.0
9	2.8552922512419974E-4	0.0	0.0	5.490946637003841E-6	0.0
10	2.910201717612036E-4	0.0	0.0	5.490946637003841E-6	0.0
11	2.965111183982074E-4	0.0	0.0	5.490946637003841E-6	0.0
12	3.2396585158322663E-4	0.0	0.0	5.490946637003841E-6	0.0
13	6.094950767074264E-4	0.0	0.0	5.490946637003841E-6	5.490946637003841E-6
14	6.534226498034571E-4	0.0	0.0	5.490946637003841E-6	1.0981893274007682E-5
15	8.730605152836106E-4	0.0	0.0	5.490946637003841E-6	1.0981893274007682E-5
16	9.444428215646606E-4	0.0	0.0	5.490946637003841E-6	1.0981893274007682E-5
17	9.554247148386683E-4	0.0	0.0	5.490946637003841E-6	1.0981893274007682E-5
18	0.0011640806870448143	0.0	0.0	1.0981893274007682E-5	1.0981893274007682E-5
19	0.0012299720466888604	0.0	0.0	1.0981893274007682E-5	1.0981893274007682E-5
20	0.0012793905664218949	0.0	0.0	1.0981893274007682E-5	1.0981893274007682E-5
21	0.0013617547659769526	0.0	0.0	1.0981893274007682E-5	1.0981893274007682E-5
22	0.0014056823390729833	0.0	0.0	1.0981893274007682E-5	1.0981893274007682E-5
23	0.0014770646453540332	0.0	0.0	1.0981893274007682E-5	1.0981893274007682E-5
24	0.0014935374852650448	5.490946637003841E-6	0.0	1.0981893274007682E-5	1.0981893274007682E-5
25	0.0015429560049980793	5.490946637003841E-6	0.0	1.0981893274007682E-5	1.0981893274007682E-5
26	0.001652774937738156	5.490946637003841E-6	0.0	1.0981893274007682E-5	1.0981893274007682E-5
27	0.0018010304969372597	5.490946637003841E-6	0.0	1.0981893274007682E-5	1.0981893274007682E-5
28	0.0018339761767592827	5.490946637003841E-6	0.0	1.592374524731114E-4	1.0981893274007682E-5
29	0.0019437951094993596	5.490946637003841E-6	0.0	1.592374524731114E-4	1.0981893274007682E-5
30	0.0020536140422394364	5.490946637003841E-6	0.0	2.635654385761844E-4	1.0981893274007682E-5
31	0.0022293243346235593	5.490946637003841E-6	0.0	2.635654385761844E-4	1.0981893274007682E-5
32	0.002383070840459667	5.490946637003841E-6	0.0	2.635654385761844E-4	1.0981893274007682E-5
33	0.0024928897731997437	5.490946637003841E-6	0.0	2.635654385761844E-4	1.0981893274007682E-5
34	0.002591726812665813	5.490946637003841E-6	0.0	2.635654385761844E-4	1.0981893274007682E-5
35	0.0026740910122208706	5.490946637003841E-6	0.0	2.635654385761844E-4	1.0981893274007682E-5
36	0.002800382784871959	5.490946637003841E-6	0.0	2.7454733185019206E-4	1.0981893274007682E-5
37	0.0031957309427362354	5.490946637003841E-6	0.0	2.7454733185019206E-4	1.0981893274007682E-5
38	0.0035361696342304733	5.490946637003841E-6	0.0	2.7454733185019206E-4	1.0981893274007682E-5
39	0.003689916140066581	5.490946637003841E-6	0.0	2.7454733185019206E-4	1.6472839911011524E-5
40	0.003832680752628681	5.490946637003841E-6	0.0	2.7454733185019206E-4	1.6472839911011524E-5
41	0.003964463471916773	5.490946637003841E-6	0.0	2.800382784871959E-4	1.6472839911011524E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	314460	0.0	45.82896	1
AGCAGTG	325030	0.0	44.3827	2
GCAGTGG	327835	0.0	44.000805	3
AGTGGTA	329350	0.0	43.572216	5
GAGTACT	155950	0.0	43.449757	20
GTGGTAT	329665	0.0	43.42936	6
TGGTATC	330510	0.0	43.302685	7
TCAACGC	332485	0.0	43.299908	12
CAACGCA	333310	0.0	43.29214	13
GGTATCA	331645	0.0	43.275654	8
GTATCAA	332025	0.0	43.267883	9
ATCAACG	333310	0.0	43.215996	11
AACGCAG	333635	0.0	43.20912	14
CAGTGGT	332215	0.0	43.203526	4
ACGCAGA	333520	0.0	43.197243	15
CGCAGAG	333580	0.0	43.111977	16
GTACTTT	156665	0.0	42.975456	22
TATCAAC	334545	0.0	42.953903	10
AGTACTT	157260	0.0	42.93091	21
AGAGTAC	328425	0.0	42.86849	19
>>END_MODULE