FastQCFastQC Report
Thu 26 May 2016
SRR805388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21700901
Sequences flagged as poor quality0
Sequence length46
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT7764533.5779758637671315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC459980.21196354934755937No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC367270.1692418208810777No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG219190.1010050227868419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGA1230750.039.60674312
CAACGAA1245100.039.48598513
AACGAAG1265750.039.3347814
CGAAGAG1268650.039.25747716
AAGCAGT4304250.039.2221641
ACGAAGA1279350.038.94008615
AGCAGTG4396200.038.3349272
AGTGGTA4422500.038.12645
GTGGTAT4423800.038.041056
GCAGTGG4441400.038.0091863
ATCAACG4444100.037.9739411
AAGAGTA1304350.037.9514718
TGGTATC4424050.037.9462287
CAGTGGT4440650.037.9453474
GGTATCA4425500.037.909848
GAAGAGT1312150.037.89048417
GTATCAA4439150.037.8387769
AGAGTAC4451450.037.8191419
TATCAAC4449700.037.7989510
GAGTACT1375850.037.66253720