##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805388_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21700901
Sequences flagged as poor quality	0
Sequence length	46
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.896132515419524	37.0	35.0	37.0	35.0	37.0
2	35.88255542938056	37.0	35.0	37.0	35.0	37.0
3	37.6671415163822	39.0	37.0	39.0	35.0	39.0
4	37.736200722725755	39.0	38.0	39.0	35.0	39.0
5	37.75677235705559	39.0	38.0	39.0	35.0	39.0
6	37.668070095338436	39.0	37.0	39.0	35.0	39.0
7	37.67638486531043	39.0	37.0	39.0	35.0	39.0
8	38.625374034009006	40.0	39.0	40.0	36.0	40.0
9	38.64201712177757	40.0	39.0	40.0	36.0	40.0
10	38.42314929688864	40.0	38.0	40.0	36.0	40.0
11	38.63373235056001	40.0	39.0	40.0	36.0	40.0
12	38.669594317765885	40.0	39.0	40.0	36.0	40.0
13	38.70275423126441	40.0	39.0	40.0	36.0	40.0
14	38.67741164295436	40.0	39.0	40.0	36.0	40.0
15	38.69662577604497	40.0	39.0	40.0	36.0	40.0
16	38.750548375848545	40.0	39.0	40.0	36.0	40.0
17	38.746374816418914	40.0	39.0	40.0	36.0	40.0
18	27.309594795165417	39.0	3.0	40.0	3.0	40.0
19	31.63885891189495	39.0	18.0	40.0	17.0	40.0
20	35.76259967270483	39.0	32.0	40.0	30.0	40.0
21	37.45768339296143	39.0	37.0	40.0	34.0	40.0
22	38.045551011914206	39.0	38.0	40.0	35.0	40.0
23	37.89957813272361	40.0	38.0	40.0	34.0	40.0
24	38.13396199540286	40.0	38.0	40.0	35.0	40.0
25	38.3201924657414	40.0	38.0	40.0	35.0	40.0
26	38.281528218574884	40.0	38.0	40.0	35.0	40.0
27	38.32216556354042	40.0	38.0	40.0	35.0	40.0
28	38.09108202465879	40.0	38.0	40.0	34.0	40.0
29	38.12218815246427	40.0	38.0	40.0	34.0	40.0
30	38.11784870130507	40.0	38.0	40.0	34.0	40.0
31	37.97599684916308	40.0	38.0	40.0	34.0	40.0
32	37.79927732032878	40.0	38.0	40.0	33.0	40.0
33	37.605169849860154	40.0	38.0	40.0	33.0	40.0
34	37.50636224735553	40.0	38.0	40.0	33.0	40.0
35	26.396529756990276	38.0	3.0	40.0	3.0	40.0
36	30.055475853283696	38.0	15.0	40.0	15.0	40.0
37	29.84921105349497	37.0	26.0	40.0	3.0	40.0
38	32.75217052047747	38.0	30.0	40.0	15.0	40.0
39	34.74913981682143	39.0	31.0	40.0	23.0	40.0
40	26.11717725452966	37.0	3.0	40.0	3.0	40.0
41	29.657291095885835	37.0	15.0	40.0	12.0	40.0
42	32.93557373493386	37.0	27.0	40.0	22.0	40.0
43	30.383211784616684	37.0	30.0	40.0	3.0	40.0
44	32.65258152184557	38.0	31.0	40.0	12.0	40.0
45	34.733247343048106	39.0	31.0	40.0	22.0	40.0
46	35.7601501430747	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	9.0
11	17.0
12	50.0
13	127.0
14	313.0
15	722.0
16	1579.0
17	2982.0
18	5607.0
19	9187.0
20	15263.0
21	24207.0
22	37940.0
23	58446.0
24	87165.0
25	126327.0
26	178519.0
27	250868.0
28	354671.0
29	544134.0
30	1093894.0
31	2338683.0
32	887424.0
33	964970.0
34	1737123.0
35	765693.0
36	678534.0
37	1100580.0
38	2533648.0
39	7902219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.56595244593761	45.047143434274915	18.83028727701214	13.556616842775329
2	21.90945896670373	42.2032661224527	19.79062067515077	16.096654235692796
3	39.22668925128961	21.803744462038697	20.523322971705184	18.446243314966505
4	19.860866606414177	23.428225399489172	19.82987250160719	36.88103549248946
5	19.127344067419134	42.10180950551316	19.816297028404488	18.954549398663218
6	38.13216787634762	21.6102133270872	22.337344426390406	17.92027437017477
7	18.409945282917057	21.863313417263182	40.80858670338158	18.918154596438185
8	38.217205820163876	21.85100056444661	21.530599121206993	18.401194494182523
9	38.05678851767491	22.526774349138776	21.352449836069017	18.063987297117297
10	18.030435694812855	22.737143494641074	41.57073478193371	17.661686028612362
11	18.110072941211058	41.9249689217973	22.02916828199898	17.935789854992656
12	18.160411864926715	22.048116803998138	41.61226301156805	18.179208319507104
13	18.211156301759086	22.33583757651353	21.937499277103747	37.515506844623644
14	18.05515356251798	41.683025050434544	21.917034689020515	18.34478669802696
15	18.31077889346622	41.33128389461802	21.8501019842448	18.507835227670963
16	18.487250828894155	21.933775929395743	21.745792951177464	37.83318029053264
17	37.83965006798566	21.92725546280313	21.880870291975434	18.352224177235772
18	14.99587505606334	43.82469649532063	15.463772679300275	25.715655769315752
19	18.72580313600804	41.544275051068155	21.666105015639673	18.063816797284133
20	38.03161444771349	21.985501892294703	21.887017502176523	18.09586615781529
21	18.591426226957118	41.272636560113334	21.94900571179049	18.186931501139053
22	38.031923190654624	21.824172185293136	22.0468864403372	18.097018183715043
23	18.416640857446424	21.981884530969474	41.21681399311485	18.38466061846925
24	18.326216040522926	41.3032205437	21.708868217038546	18.661695198738524
25	18.507862876292556	21.91700704039892	21.912260693691934	37.66286938961659
26	18.763538896380386	35.13081323213262	27.895956946672403	18.2096909248146
27	18.93514006630416	21.63054888826966	41.203998857005985	18.230312188420193
28	32.66980942404189	21.558141756418316	27.66360253889919	18.108446280640607
29	32.33470813032141	21.70496515329018	27.74966348171442	18.210663234673987
30	31.971511228957727	21.754165875416877	27.86376934303327	18.410553552592123
31	26.230887832721784	23.883197292130866	29.22114155536676	20.664773319780593
32	23.468827400300107	26.31927125975092	30.25647183957938	19.955429500369593
33	23.274162671863255	25.454855538025818	30.381291541765936	20.889690248344987
34	22.672109328548157	24.82669267971869	31.061991389205453	21.4392066025277
35	16.05328276461885	47.73106886207167	21.505061011061247	14.710587362248232
36	21.715992345202626	25.660547458375117	31.236173097144675	21.387287099277582
37	18.432336058304678	38.88021976599036	25.549063608004108	17.138380567700853
38	22.69261999766738	25.145255489622297	31.193912179038097	20.968212333672227
39	22.57567554453154	25.06204235483126	31.080110452556788	21.28217164808042
40	14.65705041463486	50.12640719387642	20.67937179198228	14.537170599506444
41	22.060355927157126	26.05769686705635	30.74532711798464	21.136620087801887
42	22.694606090318555	25.8545808766189	30.790772235678144	20.660040797384404
43	18.129993773069604	38.91166085684645	25.771533633557425	17.186811736526515
44	22.023288341806637	25.270208826813228	31.208206516402242	21.49829631497789
45	22.11843185681553	24.967663785019802	31.10275928174595	21.81114507641872
46	22.65658001941947	25.004491748983142	30.719010238330657	21.619917993266732
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5874.0
1	4285.0
2	2696.0
3	2495.5
4	2295.0
5	2577.0
6	2859.0
7	2859.0
8	3821.5
9	4784.0
10	6780.5
11	8777.0
12	12905.5
13	17034.0
14	25484.0
15	33934.0
16	47848.0
17	61762.0
18	83731.5
19	105701.0
20	105701.0
21	139026.5
22	172352.0
23	371747.0
24	571142.0
25	905318.0
26	1239494.0
27	872973.0
28	506452.0
29	538055.0
30	569658.0
31	569658.0
32	637400.0
33	705142.0
34	786755.0
35	868368.0
36	962209.5
37	1056051.0
38	1161218.5
39	1266386.0
40	1384717.5
41	1503049.0
42	1602773.5
43	1702498.0
44	1702498.0
45	1784244.5
46	1865991.0
47	1867872.5
48	1869754.0
49	1822826.0
50	1775898.0
51	1669151.5
52	1562405.0
53	1420204.0
54	1278003.0
55	1156089.0
56	1034175.0
57	1034175.0
58	863764.5
59	693354.0
60	581557.5
61	469761.0
62	391921.5
63	314082.0
64	255061.5
65	196041.0
66	157504.0
67	118967.0
68	91166.5
69	63366.0
70	63366.0
71	46127.5
72	28889.0
73	20749.0
74	12609.0
75	8972.0
76	5335.0
77	4049.5
78	2764.0
79	2125.5
80	1487.0
81	1487.0
82	1196.0
83	905.0
84	689.0
85	473.0
86	358.0
87	243.0
88	143.0
89	43.0
90	34.5
91	26.0
92	18.0
93	10.0
94	10.0
95	9.5
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	2.1700901E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.082103503648874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.03654323350493	41.33170640943942
2	14.140141608303788	15.577374872496828
3	4.422558328532116	7.308114468093911
4	2.1180351351580495	4.666633221565624
5	1.2463350149005537	3.432537714548703
6	0.7813252694545575	2.5822223617267372
7	0.5482694219084218	2.113988313181179
8	0.360515178017794	1.588634748017002
9	0.26636946457804084	1.320497137628926
>10	0.9853238883913034	9.384513108953541
>50	0.06090743795708919	2.298987152271079
>100	0.03210417242313254	3.123795027340346
>500	0.0011021386965497505	0.4057905640244087
>1k	3.741425607432823E-4	0.39272861242360235
>5k	5.2126698030310794E-5	0.19361783534901475
>10k+	4.343891502524472E-5	4.278858452939591
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	776453	3.5779758637671315	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	45998	0.21196354934755937	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	36727	0.1692418208810777	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG	21919	0.1010050227868419	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.377698419065642E-4	0.0	0.0	0.0	0.0
2	4.469860491045971E-4	0.0	0.0	0.0	0.0
3	4.5620225630263E-4	0.0	0.0	0.0	0.0
4	4.5620225630263E-4	0.0	0.0	4.608103599016465E-6	4.608103599016465E-6
5	4.608103599016465E-4	0.0	0.0	1.843241439606586E-5	4.608103599016465E-6
6	4.884589814957452E-4	0.0	0.0	2.3040517995082323E-5	4.608103599016465E-6
7	4.884589814957452E-4	0.0	0.0	3.686482879213172E-5	4.608103599016465E-6
8	4.930670850947618E-4	9.21620719803293E-6	0.0	3.686482879213172E-5	4.608103599016465E-6
9	4.930670850947618E-4	9.21620719803293E-6	0.0	1.428512115695104E-4	4.608103599016465E-6
10	5.16107603089844E-4	9.21620719803293E-6	0.0	1.428512115695104E-4	4.608103599016465E-6
11	5.25323810287877E-4	9.21620719803293E-6	0.0	1.428512115695104E-4	9.21620719803293E-6
12	5.345400174859099E-4	9.21620719803293E-6	0.0	1.428512115695104E-4	9.21620719803293E-6
13	5.667967426790252E-4	9.21620719803293E-6	0.0	1.428512115695104E-4	1.843241439606586E-5
14	5.760129498770581E-4	1.843241439606586E-5	0.0	1.428512115695104E-4	1.843241439606586E-5
15	5.990534678721404E-4	1.843241439606586E-5	0.0	1.428512115695104E-4	1.843241439606586E-5
16	6.267020894662392E-4	1.843241439606586E-5	0.0	1.4745931516852687E-4	2.3040517995082323E-5
17	6.405264002632886E-4	1.843241439606586E-5	0.0	1.4745931516852687E-4	2.3040517995082323E-5
18	6.635669182583709E-4	2.3040517995082323E-5	0.0	1.566755223665598E-4	2.3040517995082323E-5
19	6.958236434514861E-4	2.7648621594098788E-5	0.0	1.566755223665598E-4	2.7648621594098788E-5
20	7.142560578475521E-4	2.7648621594098788E-5	4.608103599016465E-6	1.566755223665598E-4	2.7648621594098788E-5
21	7.326884722436179E-4	2.7648621594098788E-5	4.608103599016465E-6	1.566755223665598E-4	2.7648621594098788E-5
22	7.695533010357496E-4	2.7648621594098788E-5	4.608103599016465E-6	1.6128362596557627E-4	2.7648621594098788E-5
23	8.018100262288649E-4	3.225672519311525E-5	4.608103599016465E-6	1.6128362596557627E-4	2.7648621594098788E-5
24	8.110262334268978E-4	3.225672519311525E-5	4.608103599016465E-6	1.8432414396065859E-4	2.7648621594098788E-5
25	8.61715373016079E-4	3.225672519311525E-5	4.608103599016465E-6	1.8432414396065859E-4	2.7648621594098788E-5
26	8.939720982091942E-4	3.225672519311525E-5	4.608103599016465E-6	2.442294907478726E-4	2.7648621594098788E-5
27	9.354450306003424E-4	3.225672519311525E-5	4.608103599016465E-6	2.442294907478726E-4	2.7648621594098788E-5
28	9.630936521944412E-4	3.225672519311525E-5	4.608103599016465E-6	2.442294907478726E-4	3.225672519311525E-5
29	0.0010091746881846057	3.225672519311525E-5	4.608103599016465E-6	2.442294907478726E-4	3.225672519311525E-5
30	0.0010644719313728033	3.225672519311525E-5	4.608103599016465E-6	2.442294907478726E-4	4.147293239114818E-5
31	0.0011520258997541161	3.225672519311525E-5	4.608103599016465E-6	2.442294907478726E-4	4.147293239114818E-5
32	0.001248796075333462	3.225672519311525E-5	4.608103599016465E-6	2.534456979459056E-4	4.6081035990164646E-5
33	0.0014008634941010054	3.225672519311525E-5	4.608103599016465E-6	2.58053801544922E-4	4.6081035990164646E-5
34	0.0014930255660813346	3.225672519311525E-5	4.608103599016465E-6	2.626619051439385E-4	4.6081035990164646E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGA	123075	0.0	39.606743	12
CAACGAA	124510	0.0	39.485985	13
AACGAAG	126575	0.0	39.33478	14
CGAAGAG	126865	0.0	39.257477	16
AAGCAGT	430425	0.0	39.222164	1
ACGAAGA	127935	0.0	38.940086	15
AGCAGTG	439620	0.0	38.334927	2
AGTGGTA	442250	0.0	38.1264	5
GTGGTAT	442380	0.0	38.04105	6
GCAGTGG	444140	0.0	38.009186	3
ATCAACG	444410	0.0	37.97394	11
AAGAGTA	130435	0.0	37.95147	18
TGGTATC	442405	0.0	37.946228	7
CAGTGGT	444065	0.0	37.945347	4
GGTATCA	442550	0.0	37.90984	8
GAAGAGT	131215	0.0	37.890484	17
GTATCAA	443915	0.0	37.838776	9
AGAGTAC	445145	0.0	37.81914	19
TATCAAC	444970	0.0	37.79895	10
GAGTACT	137585	0.0	37.662537	20
>>END_MODULE