FastQCFastQC Report
Thu 26 May 2016
SRR805387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10738439
Sequences flagged as poor quality0
Sequence length46
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT6012445.598988828823258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC395610.3684055010230071No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC211830.19726330800966507No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG126150.11747517492998749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGA1026800.039.84417712
CAACGAA1032700.039.71143313
AACGAAG1053150.039.6106914
CGAAGAG1054050.039.60343616
AAGCAGT3423150.039.425091
ACGAAGA1059700.039.3941715
AGCAGTG3459850.038.9525572
GAAGAGT1073550.038.8188717
AAGAGTA1072200.038.72038718
GCAGTGG3491450.038.620063
AGTGGTA3484350.038.6074875
ATCAACG3484200.038.58733411
GTGGTAT3486150.038.5566
AGAGTAC3481450.038.53566719
CAGTGGT3490100.038.526694
TGGTATC3472000.038.5046127
GAGTACT1070950.038.47238520
GGTATCA3480400.038.4662678
GTATCAA3486250.038.4522069
TATCAAC3487600.038.42710