##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805387_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10738439
Sequences flagged as poor quality	0
Sequence length	46
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.41576880960073	35.0	35.0	35.0	33.0	36.0
2	35.528826768955895	36.0	35.0	37.0	35.0	37.0
3	37.958884620008554	39.0	39.0	39.0	35.0	39.0
4	38.0110362409285	39.0	39.0	39.0	35.0	39.0
5	38.04082911864564	39.0	39.0	39.0	35.0	39.0
6	38.01244128685743	39.0	39.0	39.0	35.0	39.0
7	37.912099421526726	39.0	38.0	39.0	35.0	39.0
8	38.85245155278155	40.0	39.0	40.0	36.0	40.0
9	38.95232361053594	40.0	40.0	40.0	37.0	40.0
10	38.52233150460695	40.0	38.0	40.0	36.0	40.0
11	38.850692824161875	40.0	39.0	40.0	36.0	40.0
12	38.95034092012815	40.0	39.0	40.0	37.0	40.0
13	38.90986818475199	40.0	40.0	40.0	37.0	40.0
14	38.99416125565364	40.0	40.0	40.0	37.0	40.0
15	39.010444814185746	40.0	40.0	40.0	37.0	40.0
16	39.026755937245625	40.0	40.0	40.0	37.0	40.0
17	39.05259004590891	40.0	40.0	40.0	37.0	40.0
18	26.571966186146796	39.0	3.0	40.0	3.0	40.0
19	31.10699571883772	39.0	18.0	40.0	17.0	40.0
20	35.88667337962249	39.0	32.0	40.0	30.0	40.0
21	37.64179542296604	39.0	37.0	40.0	34.0	40.0
22	38.34353009781031	39.0	38.0	40.0	36.0	40.0
23	37.925124126514106	39.0	38.0	40.0	34.0	40.0
24	38.31303991203936	40.0	38.0	40.0	35.0	40.0
25	38.57374260821335	40.0	39.0	40.0	36.0	40.0
26	38.52411472468205	40.0	39.0	40.0	36.0	40.0
27	38.59907319862784	40.0	39.0	40.0	36.0	40.0
28	38.40747812601068	40.0	39.0	40.0	35.0	40.0
29	38.438160518488765	40.0	39.0	40.0	35.0	40.0
30	38.44179335562646	40.0	39.0	40.0	35.0	40.0
31	38.19125628967115	40.0	38.0	40.0	35.0	40.0
32	38.05283216676092	40.0	38.0	40.0	34.0	40.0
33	37.76459092424886	40.0	38.0	40.0	33.0	40.0
34	37.61928535423072	40.0	38.0	40.0	33.0	40.0
35	25.52390575576208	38.0	3.0	40.0	3.0	40.0
36	29.422175513591874	38.0	15.0	40.0	15.0	40.0
37	28.757443330450542	35.0	23.0	40.0	3.0	40.0
38	31.9964636387095	37.0	26.0	40.0	15.0	40.0
39	34.361843932809975	38.0	30.0	40.0	22.0	40.0
40	25.10541699775917	35.0	3.0	40.0	3.0	40.0
41	28.890216166427912	35.0	15.0	40.0	12.0	40.0
42	32.54966881126764	35.0	27.0	40.0	22.0	40.0
43	29.430843160723825	37.0	26.0	40.0	3.0	40.0
44	31.915019585248842	38.0	27.0	40.0	12.0	40.0
45	34.30396187006324	38.0	30.0	40.0	22.0	40.0
46	35.51696536153905	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	14.0
13	39.0
14	119.0
15	269.0
16	599.0
17	1169.0
18	1942.0
19	3195.0
20	5256.0
21	8660.0
22	14464.0
23	22560.0
24	36289.0
25	54564.0
26	79171.0
27	116040.0
28	173680.0
29	287187.0
30	666061.0
31	1320457.0
32	367509.0
33	459064.0
34	806102.0
35	342076.0
36	287499.0
37	524399.0
38	1400844.0
39	3759205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.507400842897185	54.832671676022926	18.53590638266884	13.124021098411045
2	18.24650677812669	55.35048436742063	13.06172154071928	13.341287313733401
3	50.10533653913758	19.557647065835173	15.87527758922875	14.461738805798497
4	17.19224740206654	18.46561683686055	16.512735230884115	47.82940053018879
5	15.964461873834734	50.346963837108916	18.448314508281882	15.240259780774467
6	47.86156535414505	18.517011643871143	17.57201395845337	16.049409043530442
7	17.170931454748683	18.21503106736463	49.43829359183397	15.175743886052711
8	47.62335568512332	19.19477309504668	18.143149111337316	15.038722108492678
9	47.38009872757111	19.030196102059154	18.395280729349956	15.19442444101978
10	15.680817295698194	19.155698514467513	49.79070980428347	15.372774385550825
11	15.391166257963565	50.42304565868465	18.60410996421361	15.581678119138173
12	15.476551107660994	19.02128419223688	49.86471497393616	15.637449726165972
13	15.705150441325783	19.26158913786259	18.405375306410924	46.627885114400705
14	15.488573339197625	50.7091021330009	18.307856477091317	15.494468050710164
15	15.674922584185651	50.373494695085576	18.280282636982896	15.671300083745878
16	15.691526487229662	18.87095508015644	18.359884523253335	47.077633909360564
17	47.14489694451866	18.72963100130289	18.529248059238405	15.596223994940047
18	13.904404541479446	42.57018175546744	12.959872473084774	30.56554122996834
19	15.839220206959318	50.398898759866306	18.36962523137674	15.392255801797635
20	47.358596533444015	18.960679480509224	18.44877081296453	15.231953173082232
21	15.904965330622076	50.131681150305	18.621849972793996	15.341503546278934
22	47.052155345856136	18.82934754297156	18.718754187643103	15.399742923529203
23	15.739326730821862	18.862583286080966	49.82721417889509	15.57087580420208
24	15.61149623329797	50.02593952435731	18.505473654038543	15.857090588306178
25	15.654202626657376	18.90632334923167	18.730785731520196	46.70868829259076
26	16.03544053283722	40.1813615554365	28.331408317354136	15.451789594372142
27	16.29966888111019	18.558479495949083	49.74550770368021	15.396343919260518
28	38.06933205096197	18.463838179832283	28.13329758636241	15.333532182843335
29	37.82256434105553	18.560025344465803	28.183360728686917	15.434049585791755
30	37.39923465598678	18.61866515235594	28.264294279643437	15.71780591201384
31	27.68706885609724	22.016142197203894	30.68960954194553	19.607179404753335
32	23.189115289475502	26.204795687715876	32.46645997616599	18.13962904664263
33	22.93918138381193	24.811790615004657	32.621789815074614	19.6272381861088
34	22.014400789537476	23.755501148723756	33.713689671282765	20.516408390456007
35	15.346643958214038	48.74593970315425	22.3756916624474	13.531724676184313
36	20.637282569654676	25.094829891011162	33.849286660752085	20.418600878582076
37	17.18671587183202	40.43935994794029	26.771656476327703	15.60226770389998
38	22.12420259592665	24.192650347038335	33.851288813951456	19.831858243083563
39	22.05138009351266	24.08833350918136	33.66207136810108	20.198215029204896
40	13.368265164052243	51.92187616840772	21.305228813983113	13.404629853556926
41	21.19891913526724	25.54355432852019	33.232418603858534	20.025107932354043
42	22.034021890891218	25.213515670201225	33.28050753000506	19.471954908902493
43	16.815349046542053	40.52321757380193	27.085426475859293	15.576006903796724
44	21.02847536778856	24.433514033091775	33.88200091279561	20.656009686324055
45	21.1838238313781	23.943759423506528	33.808256488675866	21.064160256439507
46	21.90702019166845	24.036221651955188	33.17506389895217	20.881694257424193
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2149.0
1	1488.5
2	828.0
3	742.5
4	657.0
5	760.5
6	864.0
7	864.0
8	1107.0
9	1350.0
10	2126.0
11	2902.0
12	4448.0
13	5994.0
14	9492.0
15	12990.0
16	18621.0
17	24252.0
18	33728.0
19	43204.0
20	43204.0
21	58461.0
22	73718.0
23	224436.5
24	375155.0
25	612939.0
26	850723.0
27	547996.0
28	245269.0
29	248834.0
30	252399.0
31	252399.0
32	282214.5
33	312030.0
34	350780.0
35	389530.0
36	437176.5
37	484823.0
38	541992.5
39	599162.0
40	673119.5
41	747077.0
42	806622.0
43	866167.0
44	866167.0
45	917521.0
46	968875.0
47	965443.5
48	962012.0
49	928594.0
50	895176.0
51	830264.5
52	765353.0
53	682100.0
54	598847.0
55	533611.0
56	468375.0
57	468375.0
58	382042.0
59	295709.0
60	245090.0
61	194471.0
62	160881.5
63	127292.0
64	102800.0
65	78308.0
66	62830.5
67	47353.0
68	36011.5
69	24670.0
70	24670.0
71	17954.5
72	11239.0
73	7994.0
74	4749.0
75	3469.5
76	2190.0
77	1647.5
78	1105.0
79	864.0
80	623.0
81	623.0
82	525.5
83	428.0
84	320.0
85	212.0
86	175.5
87	139.0
88	93.5
89	48.0
90	29.0
91	10.0
92	8.5
93	7.0
94	7.0
95	5.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.0738439E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.330458546710325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6727117140105	46.25003554770791
2	10.211776768130692	11.708916893871525
3	3.3440321889208424	5.7514469635737395
4	1.8159533114940805	4.164377441894909
5	1.1798634359648112	3.382105590317991
6	0.781252083552478	2.6873724114381843
7	0.4945542053536153	1.9847113558388692
8	0.32696834078377374	1.4996195925912634
9	0.21888159360487705	1.129372391592207
>10	0.8698144665648759	8.658723031050435
>50	0.054814568262164645	2.183197072232454
>100	0.027656668265460444	2.687362382926954
>500	0.0011127501722518992	0.4319833563275426
>1k	5.03692676641606E-4	0.6483467651170152
>5k	3.473741428592198E-5	0.11502972675132192
>10k+	6.947482857184395E-5	6.717399476767835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	601244	5.598988828823258	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	39561	0.3684055010230071	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	21183	0.19726330800966507	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG	12615	0.11747517492998749	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7693446878079766E-4	0.0	0.0	0.0	0.0
2	1.9555914970509214E-4	0.0	0.0	0.0	9.312340462147246E-6
3	1.9555914970509214E-4	0.0	0.0	0.0	9.312340462147246E-6
4	3.5386893756159533E-4	0.0	0.0	0.0	9.312340462147246E-6
5	3.5386893756159533E-4	0.0	0.0	0.0	9.312340462147246E-6
6	3.724936184858898E-4	9.312340462147246E-6	0.0	9.312340462147246E-6	9.312340462147246E-6
7	3.724936184858898E-4	9.312340462147246E-6	0.0	9.312340462147246E-6	9.312340462147246E-6
8	3.724936184858898E-4	9.312340462147246E-6	0.0	9.312340462147246E-6	9.312340462147246E-6
9	3.8180595894803706E-4	9.312340462147246E-6	0.0	1.3968510693220868E-4	9.312340462147246E-6
10	4.004306398723315E-4	9.312340462147246E-6	0.0	1.3968510693220868E-4	9.312340462147246E-6
11	4.004306398723315E-4	9.312340462147246E-6	0.0	1.3968510693220868E-4	9.312340462147246E-6
12	4.19055320796626E-4	9.312340462147246E-6	0.0	1.3968510693220868E-4	9.312340462147246E-6
13	4.283676612587733E-4	9.312340462147246E-6	0.0	1.3968510693220868E-4	9.312340462147246E-6
14	4.6561702310736225E-4	1.8624680924294492E-5	0.0	1.3968510693220868E-4	9.312340462147246E-6
15	4.93554044493804E-4	2.7937021386441734E-5	0.0	1.3968510693220868E-4	9.312340462147246E-6
16	4.93554044493804E-4	4.656170231073622E-5	0.0	1.3968510693220868E-4	9.312340462147246E-6
17	5.028663849559513E-4	4.656170231073622E-5	0.0	1.3968510693220868E-4	9.312340462147246E-6
18	5.214910658802457E-4	4.656170231073622E-5	0.0	1.3968510693220868E-4	1.8624680924294492E-5
19	5.401157468045402E-4	4.656170231073622E-5	0.0	1.3968510693220868E-4	1.8624680924294492E-5
20	5.68052768190982E-4	4.656170231073622E-5	0.0	1.3968510693220868E-4	1.8624680924294492E-5
21	5.959897895774237E-4	4.656170231073622E-5	0.0	1.4899744739435593E-4	1.8624680924294492E-5
22	6.518638323503072E-4	4.656170231073622E-5	0.0	1.4899744739435593E-4	1.8624680924294492E-5
23	6.891131941988961E-4	4.656170231073622E-5	0.0	1.4899744739435593E-4	1.8624680924294492E-5
24	7.263625560474851E-4	4.656170231073622E-5	0.0	1.862468092429449E-4	1.8624680924294492E-5
25	7.822365988203686E-4	4.656170231073622E-5	0.0	1.862468092429449E-4	1.8624680924294492E-5
26	8.00861279744663E-4	4.656170231073622E-5	9.312340462147246E-6	3.911182994101843E-4	1.8624680924294492E-5
27	8.38110641593252E-4	4.656170231073622E-5	9.312340462147246E-6	3.911182994101843E-4	1.8624680924294492E-5
28	8.75360003441841E-4	4.656170231073622E-5	9.312340462147246E-6	3.911182994101843E-4	1.8624680924294492E-5
29	9.032970248282827E-4	4.656170231073622E-5	9.312340462147246E-6	3.911182994101843E-4	1.8624680924294492E-5
30	9.964204294497553E-4	4.656170231073622E-5	9.312340462147246E-6	3.911182994101843E-4	1.8624680924294492E-5
31	0.001024357450836197	4.656170231073622E-5	1.8624680924294492E-5	3.911182994101843E-4	1.8624680924294492E-5
32	0.001070919153146933	4.656170231073622E-5	1.8624680924294492E-5	3.911182994101843E-4	1.8624680924294492E-5
33	0.0011919795791548475	4.656170231073622E-5	1.8624680924294492E-5	3.911182994101843E-4	1.8624680924294492E-5
34	0.0012757906433141725	4.656170231073622E-5	1.8624680924294492E-5	3.911182994101843E-4	1.8624680924294492E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGA	102680	0.0	39.844177	12
CAACGAA	103270	0.0	39.711433	13
AACGAAG	105315	0.0	39.61069	14
CGAAGAG	105405	0.0	39.603436	16
AAGCAGT	342315	0.0	39.42509	1
ACGAAGA	105970	0.0	39.39417	15
AGCAGTG	345985	0.0	38.952557	2
GAAGAGT	107355	0.0	38.81887	17
AAGAGTA	107220	0.0	38.720387	18
GCAGTGG	349145	0.0	38.62006	3
AGTGGTA	348435	0.0	38.607487	5
ATCAACG	348420	0.0	38.587334	11
GTGGTAT	348615	0.0	38.556	6
AGAGTAC	348145	0.0	38.535667	19
CAGTGGT	349010	0.0	38.52669	4
TGGTATC	347200	0.0	38.504612	7
GAGTACT	107095	0.0	38.472385	20
GGTATCA	348040	0.0	38.466267	8
GTATCAA	348625	0.0	38.452206	9
TATCAAC	348760	0.0	38.427	10
>>END_MODULE