FastQCFastQC Report
Thu 26 May 2016
SRR805386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13031244
Sequences flagged as poor quality0
Sequence length46
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1350321.0362172636779727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGA225800.038.627112
CAACGAA228900.038.3224113
AACGAAG233950.038.03376814
CGAAGAG237400.037.52316716
AAGCAGT762850.037.192111
ACGAAGA242950.036.6742115
AGCAGTG809300.035.022862
GAAGAGT263600.033.87708717
GCAGTGG841800.033.7205963
AGTGGTA845350.033.522215
ATCAACG854150.033.1908911
GTGGTAT853700.033.1545036
CAGTGGT856750.033.0644874
GGTATCA853650.032.9830748
AAGAGTA269400.032.9621418
TGGTATC856600.032.9535377
AGAGTAC860300.032.92804719
GTATCAA862150.032.7019659
TATCAAC868400.032.54260610
GAGTACT290550.032.53140320
Produced by FastQC (version 0.11.5)