##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805386_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13031244
Sequences flagged as poor quality	0
Sequence length	46
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.02389288390272	37.0	35.0	37.0	35.0	37.0
2	35.88331988872282	37.0	35.0	37.0	35.0	37.0
3	37.61674165567002	39.0	37.0	39.0	35.0	39.0
4	37.70638144754254	39.0	37.0	39.0	35.0	39.0
5	37.73531045846429	39.0	37.0	39.0	35.0	39.0
6	37.607060538502694	39.0	37.0	39.0	35.0	39.0
7	37.688136527871016	39.0	37.0	39.0	35.0	39.0
8	38.64050907188907	40.0	39.0	40.0	36.0	40.0
9	38.606932078011894	40.0	39.0	40.0	36.0	40.0
10	38.56980116403315	40.0	39.0	40.0	36.0	40.0
11	38.68036090798392	40.0	39.0	40.0	36.0	40.0
12	38.660981177238334	40.0	39.0	40.0	36.0	40.0
13	38.665155375802954	40.0	39.0	40.0	36.0	40.0
14	38.60403949154816	40.0	39.0	40.0	36.0	40.0
15	38.62914246713514	40.0	39.0	40.0	36.0	40.0
16	38.73200540178666	40.0	39.0	40.0	36.0	40.0
17	38.71949585166236	40.0	39.0	40.0	36.0	40.0
18	26.16004274035541	38.0	3.0	40.0	3.0	40.0
19	30.86614838920981	38.0	18.0	40.0	17.0	40.0
20	35.40571452733139	38.0	32.0	40.0	30.0	40.0
21	37.38129191656606	39.0	37.0	40.0	34.0	40.0
22	37.95917803396207	39.0	38.0	40.0	34.0	40.0
23	38.14588944846708	40.0	38.0	40.0	35.0	40.0
24	38.25139081119193	40.0	38.0	40.0	35.0	40.0
25	38.35166266551374	40.0	38.0	40.0	35.0	40.0
26	38.342790066704296	40.0	38.0	40.0	35.0	40.0
27	38.32262882960368	40.0	38.0	40.0	35.0	40.0
28	38.109243522721236	40.0	38.0	40.0	34.0	40.0
29	38.134880522534914	40.0	38.0	40.0	34.0	40.0
30	38.1295450380639	40.0	38.0	40.0	34.0	40.0
31	38.10057197916024	40.0	38.0	40.0	34.0	40.0
32	37.94285480342475	40.0	38.0	40.0	34.0	40.0
33	37.86601793351425	40.0	38.0	40.0	34.0	40.0
34	37.74458347952045	40.0	38.0	40.0	33.0	40.0
35	25.369314779156923	37.0	3.0	40.0	3.0	40.0
36	29.47276798746152	37.0	15.0	40.0	15.0	40.0
37	29.17359041086177	37.0	24.0	40.0	3.0	40.0
38	32.506322189961296	38.0	30.0	40.0	15.0	40.0
39	34.709469564072315	39.0	30.0	40.0	23.0	40.0
40	25.166209150868482	37.0	3.0	40.0	3.0	40.0
41	29.1184723423182	37.0	15.0	40.0	12.0	40.0
42	32.778119494961494	37.0	27.0	40.0	22.0	40.0
43	29.790694963581373	37.0	26.0	40.0	3.0	40.0
44	32.36365515065177	39.0	28.0	40.0	12.0	40.0
45	34.7343798489231	39.0	30.0	40.0	22.0	40.0
46	35.94417171530208	39.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	10.0
12	15.0
13	49.0
14	148.0
15	343.0
16	770.0
17	1581.0
18	2880.0
19	5103.0
20	8583.0
21	13628.0
22	21217.0
23	32214.0
24	48394.0
25	70782.0
26	102035.0
27	145322.0
28	209871.0
29	331720.0
30	700163.0
31	1583128.0
32	571847.0
33	575102.0
34	1108402.0
35	494680.0
36	391184.0
37	606913.0
38	1286591.0
39	4718565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.506231945315427	31.51248645179232	25.949625377285546	20.031656225606703
2	22.223120064362238	29.253385171822426	26.87158647324845	21.651908290566887
3	26.42238914412162	26.0488791400115	25.579000746206574	21.9497309696603
4	22.05404180905522	27.053756341297884	25.458329227815856	25.43387262183104
5	23.358491330528384	31.66995415019472	24.692876597199774	20.278677922077122
6	27.643416085217957	25.78516678837416	26.08255973105868	20.488857395349207
7	21.848006222583198	26.0561462896405	31.44678282441799	20.64906466335831
8	27.087068586851725	25.975079585648153	25.929742394509685	21.008109432990434
9	26.416610724194868	26.325721473713486	26.345727238320453	20.911940563771196
10	20.76288342080004	26.45728220575104	31.995149503761883	20.78468486968704
11	20.87294965852838	31.608624625553784	26.45228651999763	21.066139195920204
12	20.986776089834557	26.22723509743199	31.685769984814954	21.1002188279185
13	21.05377660030002	26.45654551476436	26.17378663157562	26.31589125336
14	21.02156171736175	31.52392050981472	26.179281118517927	21.27523665430561
15	21.222187229400355	31.27135060935088	26.181260975544625	21.32520118570414
16	21.12622555452112	26.137504600481737	26.172842746248936	26.56342709874821
17	26.595181549819802	26.071586104902956	26.223091210631928	21.110141134645318
18	16.79553387228418	48.2122888651306	17.686131884262164	17.306045378323052
19	21.47878590869759	31.47045669622946	26.103547750314554	20.947209644758395
20	26.729550916244065	25.959839290861257	26.29699052523305	21.01361926766163
21	21.37333166350043	31.24189064374821	26.353938273276135	21.030839419475225
22	26.639536486309368	25.953370223134492	26.352656737913893	21.05443655264225
23	21.27146111299888	26.014807181877647	31.461831272593777	21.2519004325297
24	21.189734456664304	31.286491143899998	26.178168408173462	21.345605991262232
25	21.34119351920661	25.9180857944184	26.338997259202575	26.40172342717242
26	21.6661356352471	29.251835051204626	28.04922538477524	21.03280392877303
27	21.749995625897267	25.667595511219037	31.530803966221487	21.05160489666221
28	25.536080822368145	25.608406994758138	27.867515948592477	20.98799623428124
29	25.231681641445743	25.773540883740647	27.915347145675423	21.079430329138184
30	25.01803358144472	25.80948526479897	27.981718399256433	21.19076275449988
31	23.41082708604029	26.477556555613567	28.333695539735114	21.777920818611022
32	22.827007152962526	26.973656544225555	28.584124432018925	21.615211870792997
33	22.7133265250808	26.80671162323413	28.590562804287913	21.889399047397163
34	22.69591452665609	26.625992115564717	28.773292864441796	21.904800493337397
35	16.038415058454895	50.76070250852489	19.012705156928995	14.188177276091215
36	22.196177126297382	26.957602819807537	28.851857888625215	21.99436216526987
37	17.8027592760906	41.92809988056398	22.876572643409947	17.39256819993548
38	22.630916894810657	26.784979239127132	28.738837213085723	21.84526665297649
39	22.41967075438078	26.789307298673865	28.745866472916937	22.04515547402842
40	14.158970548015215	53.58139253627666	18.21916618244582	14.040470733262303
41	22.310878378150235	27.07501294580932	28.66236715389567	21.951741522144776
42	22.871661370165427	26.91119128764683	28.604590628492566	21.612556713695177
43	17.709038369629177	41.98088072021366	22.912992804063833	17.397088106093324
44	22.334191578332813	26.84935528795255	28.755957604661535	22.0604955290531
45	22.295139282174443	26.763016639086796	28.775625719232945	22.166218359505816
46	22.881721806452244	26.738414229677538	28.543967099380534	21.835896864489683
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7722.0
1	5461.0
2	3200.0
3	2968.5
4	2737.0
5	2901.5
6	3066.0
7	3066.0
8	3948.0
9	4830.0
10	6432.0
11	8034.0
12	11507.5
13	14981.0
14	21924.5
15	28868.0
16	40224.5
17	51581.0
18	69270.0
19	86959.0
20	86959.0
21	111964.0
22	136969.0
23	202876.5
24	268784.0
25	355823.5
26	442863.0
27	403951.0
28	365039.0
29	403052.0
30	441065.0
31	441065.0
32	490950.0
33	540835.0
34	596421.5
35	652008.0
36	711194.0
37	770380.0
38	824696.0
39	879012.0
40	930779.0
41	982546.0
42	1016970.0
43	1051394.0
44	1051394.0
45	1067324.5
46	1083255.0
47	1068202.0
48	1053149.0
49	1012341.0
50	971533.0
51	909505.5
52	847478.0
53	769714.0
54	691950.0
55	617534.0
56	543118.0
57	543118.0
58	467798.5
59	392479.0
60	331483.0
61	270487.0
62	226133.0
63	181779.0
64	148308.5
65	114838.0
66	92701.0
67	70564.0
68	53687.5
69	36811.0
70	36811.0
71	26943.5
72	17076.0
73	12151.0
74	7226.0
75	5171.5
76	3117.0
77	2348.5
78	1580.0
79	1261.0
80	942.0
81	942.0
82	734.0
83	526.0
84	397.5
85	269.0
86	203.5
87	138.0
88	84.0
89	30.0
90	23.0
91	16.0
92	11.5
93	7.0
94	7.0
95	5.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.3031244E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.21375211003246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.60000842489713	55.518564905576284
2	11.166302531778273	15.010581807131453
3	3.0118239663817516	6.073079684263133
4	1.1553677081466713	3.1062639492522677
5	0.5565742828949518	1.8704722940660188
6	0.32178935399608605	1.2977201922684247
7	0.20104183936455566	0.9458943448355945
8	0.13998804607773158	0.7527297461949162
9	0.10270281723536488	0.6212737528793806
>10	0.6789397457626187	8.597481159297454
>50	0.03819481016409362	1.744875362676297
>100	0.02695985011332662	3.009609987973288
>500	1.891323960517301E-4	0.0853946324751493
>1k	4.699740071898364E-5	0.06389500304001841
>5k	5.8744492202464115E-5	0.23583228913917997
>10k+	1.1748898440492825E-5	1.066330888931213
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	135032	1.0362172636779727	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.597125800115476E-4	7.673864444561088E-6	0.0	0.0	0.0
2	7.597125800115476E-4	1.5347728889122176E-5	0.0	0.0	0.0
3	7.597125800115476E-4	1.5347728889122176E-5	0.0	0.0	7.673864444561088E-6
4	7.597125800115476E-4	1.5347728889122176E-5	0.0	0.0	7.673864444561088E-6
5	7.597125800115476E-4	1.5347728889122176E-5	0.0	0.0	7.673864444561088E-6
6	7.597125800115476E-4	1.5347728889122176E-5	0.0	0.0	1.5347728889122176E-5
7	7.597125800115476E-4	1.5347728889122176E-5	0.0	9.208637333473304E-5	1.5347728889122176E-5
8	7.597125800115476E-4	1.5347728889122176E-5	0.0	9.208637333473304E-5	1.5347728889122176E-5
9	7.597125800115476E-4	1.5347728889122176E-5	0.0	2.2254206889227152E-4	1.5347728889122176E-5
10	7.673864444561087E-4	1.5347728889122176E-5	0.0	2.2254206889227152E-4	1.5347728889122176E-5
11	8.287773600125974E-4	1.5347728889122176E-5	7.673864444561088E-6	2.378897977813937E-4	1.5347728889122176E-5
12	8.287773600125974E-4	1.5347728889122176E-5	7.673864444561088E-6	2.378897977813937E-4	1.5347728889122176E-5
13	8.441250889017196E-4	1.5347728889122176E-5	7.673864444561088E-6	2.378897977813937E-4	2.302159333368326E-5
14	8.517989533462807E-4	4.604318666736652E-5	7.673864444561088E-6	2.455636622259548E-4	3.069545777824435E-5
15	8.517989533462807E-4	6.13909155564887E-5	7.673864444561088E-6	2.455636622259548E-4	3.8369322222805436E-5
16	8.748205466799639E-4	6.13909155564887E-5	7.673864444561088E-6	2.5323752667051586E-4	4.604318666736652E-5
17	8.901682755690861E-4	6.13909155564887E-5	7.673864444561088E-6	2.5323752667051586E-4	4.604318666736652E-5
18	9.285375977918915E-4	6.13909155564887E-5	7.673864444561088E-6	2.5323752667051586E-4	5.371705111192761E-5
19	9.592330555701359E-4	6.906478000104978E-5	7.673864444561088E-6	2.5323752667051586E-4	6.13909155564887E-5
20	9.822546489038192E-4	6.906478000104978E-5	7.673864444561088E-6	2.5323752667051586E-4	6.906478000104978E-5
21	0.0010052762422375025	7.673864444561087E-5	7.673864444561088E-6	2.5323752667051586E-4	6.906478000104978E-5
22	0.0010513194289048689	7.673864444561087E-5	7.673864444561088E-6	2.6091139111507697E-4	6.906478000104978E-5
23	0.0010896887511276743	8.441250889017196E-5	7.673864444561088E-6	2.6091139111507697E-4	6.906478000104978E-5
24	0.0011203842089059187	8.441250889017196E-5	7.673864444561088E-6	2.992807133378824E-4	6.906478000104978E-5
25	0.0011587535311287241	8.441250889017196E-5	7.673864444561088E-6	2.992807133378824E-4	6.906478000104978E-5
26	0.0012047967177960907	8.441250889017196E-5	7.673864444561088E-6	5.525182400083982E-4	6.906478000104978E-5
27	0.0012431660400188962	8.441250889017196E-5	7.673864444561088E-6	5.525182400083982E-4	7.673864444561087E-5
28	0.001319904684464507	8.441250889017196E-5	7.673864444561088E-6	5.525182400083982E-4	7.673864444561087E-5
29	0.0013812956000209956	8.441250889017196E-5	7.673864444561088E-6	5.525182400083982E-4	7.673864444561087E-5
30	0.0014426865155774845	8.441250889017196E-5	7.673864444561088E-6	5.525182400083982E-4	7.673864444561087E-5
31	0.001504077431133973	9.208637333473304E-5	7.673864444561088E-6	5.525182400083982E-4	7.673864444561087E-5
32	0.0016191853978023893	9.208637333473304E-5	7.673864444561088E-6	5.525182400083982E-4	8.441250889017196E-5
33	0.001849401331139222	9.208637333473304E-5	7.673864444561088E-6	5.525182400083982E-4	9.208637333473304E-5
34	0.0019184661111402719	9.208637333473304E-5	7.673864444561088E-6	5.525182400083982E-4	9.208637333473304E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGA	22580	0.0	38.6271	12
CAACGAA	22890	0.0	38.32241	13
AACGAAG	23395	0.0	38.033768	14
CGAAGAG	23740	0.0	37.523167	16
AAGCAGT	76285	0.0	37.19211	1
ACGAAGA	24295	0.0	36.67421	15
AGCAGTG	80930	0.0	35.02286	2
GAAGAGT	26360	0.0	33.877087	17
GCAGTGG	84180	0.0	33.720596	3
AGTGGTA	84535	0.0	33.52221	5
ATCAACG	85415	0.0	33.19089	11
GTGGTAT	85370	0.0	33.154503	6
CAGTGGT	85675	0.0	33.064487	4
GGTATCA	85365	0.0	32.983074	8
AAGAGTA	26940	0.0	32.96214	18
TGGTATC	85660	0.0	32.953537	7
AGAGTAC	86030	0.0	32.928047	19
GTATCAA	86215	0.0	32.701965	9
TATCAAC	86840	0.0	32.542606	10
GAGTACT	29055	0.0	32.531403	20
>>END_MODULE