FastQCFastQC Report
Thu 26 May 2016
SRR805385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12832391
Sequences flagged as poor quality0
Sequence length46
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT3755182.9263291618841727No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC286290.22309949876059731No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC205930.16047671864113244No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG168050.13095766798252953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGA620000.039.59032412
CAACGAA626550.039.4190413
AACGAAG642700.039.11622614
CGAAGAG643850.039.08363716
AAGCAGT2168400.038.6229441
ACGAAGA652350.038.59277715
AGCAGTG2220350.037.698562
GAAGAGT669450.037.54126417
AAGAGTA666500.037.5183818
GCAGTGG2252450.037.19153
AGTGGTA2248650.037.170755
ATCAACG2252900.037.0837611
GTGGTAT2250750.037.0605356
CAGTGGT2259150.036.985594
TGGTATC2248600.036.919867
GGTATCA2251500.036.8891878
AGAGTAC2259750.036.86779419
GTATCAA2257500.036.8575869
GAGTACT685050.036.77103820
TATCAAC2266050.036.7388210
Produced by FastQC (version 0.11.5)