##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805385_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12832391
Sequences flagged as poor quality	0
Sequence length	46
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.103420321279174	37.0	35.0	37.0	35.0	37.0
2	36.11492456861703	37.0	36.0	37.0	35.0	37.0
3	37.93156629968647	39.0	39.0	39.0	35.0	39.0
4	37.929537994906795	39.0	38.0	39.0	35.0	39.0
5	37.95119116928404	39.0	38.0	39.0	35.0	39.0
6	37.86792796447677	39.0	38.0	39.0	35.0	39.0
7	37.830442666530345	39.0	38.0	39.0	35.0	39.0
8	38.724887669024426	40.0	39.0	40.0	36.0	40.0
9	38.77457825279794	40.0	39.0	40.0	36.0	40.0
10	38.58858204990793	40.0	39.0	40.0	36.0	40.0
11	38.77724073401442	40.0	39.0	40.0	36.0	40.0
12	38.79296446001373	40.0	39.0	40.0	36.0	40.0
13	38.773492484759856	40.0	39.0	40.0	36.0	40.0
14	38.80569225174015	40.0	39.0	40.0	36.0	40.0
15	38.823121817282534	40.0	39.0	40.0	36.0	40.0
16	38.8709905270187	40.0	39.0	40.0	36.0	40.0
17	38.883966440860476	40.0	39.0	40.0	36.0	40.0
18	26.982743823812726	39.0	3.0	40.0	3.0	40.0
19	31.304122591027657	39.0	17.0	40.0	17.0	40.0
20	35.75607585523228	39.0	32.0	40.0	30.0	40.0
21	37.55088299600597	39.0	37.0	40.0	34.0	40.0
22	38.16798584145386	39.0	38.0	40.0	35.0	40.0
23	38.05978176631307	40.0	38.0	40.0	35.0	40.0
24	38.28325726670891	40.0	38.0	40.0	35.0	40.0
25	38.439267943129224	40.0	39.0	40.0	35.0	40.0
26	38.417686306472426	40.0	39.0	40.0	35.0	40.0
27	38.43496687406112	40.0	39.0	40.0	35.0	40.0
28	38.24213149365539	40.0	38.0	40.0	35.0	40.0
29	38.26526147777137	40.0	38.0	40.0	35.0	40.0
30	38.26600740267344	40.0	38.0	40.0	35.0	40.0
31	38.14577423646147	40.0	38.0	40.0	34.0	40.0
32	38.04603148392221	40.0	38.0	40.0	34.0	40.0
33	37.79878177028739	40.0	38.0	40.0	33.0	40.0
34	37.6545530758843	40.0	38.0	40.0	33.0	40.0
35	26.04746247211451	38.0	3.0	40.0	3.0	40.0
36	29.755309045679795	37.0	15.0	40.0	15.0	40.0
37	29.33111748231487	37.0	24.0	40.0	3.0	40.0
38	32.42826212200049	38.0	30.0	40.0	15.0	40.0
39	34.60691791576488	39.0	30.0	40.0	22.0	40.0
40	25.75272994720937	37.0	3.0	40.0	3.0	40.0
41	29.352547393544974	37.0	15.0	40.0	12.0	40.0
42	32.79104868297732	37.0	27.0	40.0	22.0	40.0
43	29.925471176805633	37.0	27.0	40.0	3.0	40.0
44	32.29987131782378	39.0	30.0	40.0	12.0	40.0
45	34.558978057947265	39.0	30.0	40.0	22.0	40.0
46	35.73523959798295	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	9.0
13	39.0
14	119.0
15	300.0
16	613.0
17	1238.0
18	2319.0
19	4128.0
20	7037.0
21	11878.0
22	19501.0
23	30481.0
24	46809.0
25	69008.0
26	98895.0
27	142287.0
28	205337.0
29	326532.0
30	717744.0
31	1517823.0
32	472621.0
33	535777.0
34	970089.0
35	431307.0
36	354897.0
37	592253.0
38	1399550.0
39	4873795.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.3118017523001	39.91482179743432	24.556312225835388	17.217064224430196
2	21.72980078303412	38.74330200817603	20.4444596490241	19.08243755976575
3	36.160463003348326	22.710514353872167	21.33530688084551	19.793715761934
4	20.184227553540097	23.83864394406311	20.96793185307399	35.009196649322796
5	21.090769444291404	39.45574133456501	21.018732985926007	18.434756235217584
6	36.75971220016597	22.788777243461485	21.908177517346534	18.54333303902601
7	20.127433772864308	22.662729026882054	38.812587615199696	18.397249585053945
8	36.03605127056992	22.864102254988957	22.326680974730273	18.77316549971085
9	35.5612527704307	22.999634284834368	22.650393056134277	18.788719888600653
10	18.862143461806923	23.206937818525013	39.22963382272251	18.70128489694555
11	18.698806792903987	39.39859687878899	23.016949842005282	18.885646486301734
12	18.7954606433049	23.171956029082967	39.07412889772451	18.958454429887617
13	18.912321172258544	23.21686582025127	22.812779005876614	35.05803400161358
14	18.872079256313185	39.43425664009147	22.58903270637561	19.104631397219737
15	19.033389802414842	39.266688491645866	22.58876775185544	19.111153954083846
16	19.064888219194692	23.01963055832697	22.57572263812722	35.33975858435112
17	35.483348348721606	22.73344850542662	22.649083869093452	19.13411927675832
18	16.006892246347544	44.43349645440199	15.907807048585099	23.651804250665368
19	19.25539831197475	39.27209668096928	22.602584350804147	18.869920656251825
20	35.60381693481753	22.928642058989627	22.707334899630162	18.760206106562684
21	19.396564521763715	38.99698816845591	22.822878448762978	18.783568861017404
22	35.40569329597267	22.855039251843245	22.937681683795326	18.801585768388758
23	19.28103655819091	22.783891170398405	38.94752739376473	18.987544877645952
24	19.00556957779731	38.979812881325074	22.846178860977663	19.16843867989995
25	19.07812035964303	22.937899881635467	22.88314780932096	35.10083194940054
26	19.458509330022753	33.76179856115669	27.81656980371	18.96312230511056
27	19.612447906239762	22.57794358042862	39.02363168329269	18.785976830038923
28	31.163163591259025	22.416017404706572	27.76069557107479	18.66012343295961
29	30.9847634786066	22.4868303966112	27.708967097402194	18.81943902738001
30	30.6758187153119	22.558718792156505	27.780060629386995	18.9854018631446
31	25.981806508233735	24.280221823041394	28.947816505902914	20.790155162821954
32	23.68337280246526	26.238516267155514	29.810313604066458	20.267797326312767
33	23.51122249937677	25.59374164954918	29.885685372273958	21.009350478800094
34	22.81105680149553	25.076963443523503	30.4972939181794	21.614685836801577
35	16.246535817058565	48.35964708369625	20.923723412106128	14.470093687139052
36	21.97102628808614	25.780121568926635	30.804828188293204	21.444023954694025
37	18.181358407797894	40.386549942251605	24.52603727551631	16.906054374434195
38	22.843833234196183	25.44106550369296	30.6589941032813	21.05610715882956
39	22.70551918188902	25.427443724244377	30.529922288059957	21.33711480580665
40	14.449286964525939	51.49018604560911	19.875345132485442	14.185181857379503
41	22.318934951405392	26.221761790144953	30.25797764422858	21.201325614221076
42	22.808547526333946	26.15103451882038	30.208875337417634	20.83154261742804
43	17.731263020274245	40.530989119642626	24.831997404069124	16.905750456014005
44	22.2538886167044	25.520668751443125	30.728895339925348	21.496547291927122
45	22.439715248701507	25.22423140005631	30.554890355195692	21.78116299604649
46	22.809732028894693	25.22997467891993	30.164604554209735	21.795688737975645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3587.0
1	2477.0
2	1367.0
3	1224.0
4	1081.0
5	1233.0
6	1385.0
7	1385.0
8	1842.5
9	2300.0
10	3321.5
11	4343.0
12	6576.0
13	8809.0
14	13685.0
15	18561.0
16	26543.5
17	34526.0
18	47299.0
19	60072.0
20	60072.0
21	80069.5
22	100067.0
23	204811.0
24	309555.0
25	481660.0
26	653765.0
27	479573.5
28	305382.0
29	328131.0
30	350880.0
31	350880.0
32	393714.0
33	436548.0
34	487997.0
35	539446.0
36	599485.5
37	659525.0
38	720760.5
39	781996.0
40	849843.5
41	917691.0
42	973431.0
43	1029171.0
44	1029171.0
45	1069677.0
46	1110183.0
47	1109566.0
48	1108949.0
49	1074689.0
50	1040429.0
51	976504.5
52	912580.0
53	826824.5
54	741069.0
55	675755.5
56	610442.0
57	610442.0
58	504470.5
59	398499.0
60	332459.5
61	266420.0
62	222159.5
63	177899.0
64	144249.0
65	110599.0
66	89337.0
67	68075.0
68	52152.0
69	36229.0
70	36229.0
71	26547.0
72	16865.0
73	12047.5
74	7230.0
75	5231.5
76	3233.0
77	2489.0
78	1745.0
79	1326.0
80	907.0
81	907.0
82	705.5
83	504.0
84	379.0
85	254.0
86	198.5
87	143.0
88	89.0
89	35.0
90	28.0
91	21.0
92	14.5
93	8.0
94	8.0
95	7.5
96	7.0
97	7.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.2832391E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.97168159329151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.88745871525698	50.12731836392419
2	11.164499131988876	13.837655707123881
3	3.6521170620934273	6.7898350714044335
4	1.5982277798799165	3.9617945235308545
5	0.7991374769294655	2.4761946634769587
6	0.4512548677941917	1.6779013790618702
7	0.2696427644246351	1.1697150878600877
8	0.1883073581260337	0.933577891156838
9	0.12947892256455348	0.722162390599166
>10	0.7777934335104009	8.500392198269239
>50	0.04777135056194665	2.0694920831581545
>100	0.03353469959661723	3.4169998339429073
>500	3.8241684461886644E-4	0.1575985868137247
>1k	3.0208410555509027E-4	0.4118084761402509
>5k	3.940128122915521E-5	0.15372530048778887
>10k+	5.2535041638873614E-5	3.5938284430497554
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	375518	2.9263291618841727	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	28629	0.22309949876059731	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	20593	0.16047671864113244	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG	16805	0.13095766798252953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.272967602062624E-4	0.0	0.0	0.0	0.0
2	3.5846788022590644E-4	0.0	0.0	0.0	7.79278000491101E-6
3	3.7405344023572846E-4	7.79278000491101E-6	0.0	0.0	7.79278000491101E-6
4	3.7405344023572846E-4	7.79278000491101E-6	0.0	0.0	2.337834001473303E-5
5	3.818462202406395E-4	7.79278000491101E-6	0.0	7.79278000491101E-6	2.337834001473303E-5
6	3.896390002455505E-4	1.558556000982202E-5	0.0	7.79278000491101E-6	2.337834001473303E-5
7	3.896390002455505E-4	1.558556000982202E-5	0.0	4.675668002946606E-5	2.337834001473303E-5
8	3.9743178025046153E-4	1.558556000982202E-5	0.0	4.675668002946606E-5	2.337834001473303E-5
9	3.9743178025046153E-4	1.558556000982202E-5	0.0	1.402700400883982E-4	2.337834001473303E-5
10	4.052245602553725E-4	1.558556000982202E-5	0.0	1.402700400883982E-4	2.337834001473303E-5
11	4.052245602553725E-4	1.558556000982202E-5	0.0	1.480628200933092E-4	2.337834001473303E-5
12	4.052245602553725E-4	1.558556000982202E-5	0.0	1.480628200933092E-4	2.337834001473303E-5
13	4.1301734026028355E-4	1.558556000982202E-5	0.0	1.480628200933092E-4	2.337834001473303E-5
14	4.3639568027501656E-4	3.896390002455505E-5	0.0	1.480628200933092E-4	2.337834001473303E-5
15	4.519812402848386E-4	3.896390002455505E-5	0.0	1.480628200933092E-4	2.337834001473303E-5
16	4.519812402848386E-4	3.896390002455505E-5	0.0	1.480628200933092E-4	3.896390002455505E-5
17	4.675668002946606E-4	3.896390002455505E-5	0.0	1.480628200933092E-4	3.896390002455505E-5
18	4.987379203143047E-4	3.896390002455505E-5	0.0	1.480628200933092E-4	3.896390002455505E-5
19	5.377018203388597E-4	3.896390002455505E-5	0.0	1.480628200933092E-4	4.675668002946606E-5
20	5.610801603535928E-4	3.896390002455505E-5	0.0	1.480628200933092E-4	4.675668002946606E-5
21	6.156296203879698E-4	3.896390002455505E-5	0.0	1.480628200933092E-4	4.675668002946606E-5
22	6.545935204125248E-4	3.896390002455505E-5	0.0	1.480628200933092E-4	4.675668002946606E-5
23	6.623863004174359E-4	3.896390002455505E-5	0.0	1.480628200933092E-4	4.675668002946606E-5
24	6.779718604272578E-4	3.896390002455505E-5	0.0	2.337834001473303E-4	4.675668002946606E-5
25	6.857646404321688E-4	3.896390002455505E-5	0.0	2.4157618015224132E-4	4.675668002946606E-5
26	7.32521320461635E-4	4.675668002946606E-5	0.0	4.519812402848386E-4	4.675668002946606E-5
27	7.558996604763679E-4	4.675668002946606E-5	0.0	4.519812402848386E-4	4.675668002946606E-5
28	8.10449120510745E-4	4.675668002946606E-5	0.0	4.519812402848386E-4	5.454946003437707E-5
29	8.961697005647662E-4	4.675668002946606E-5	0.0	4.519812402848386E-4	5.454946003437707E-5
30	9.429263805942322E-4	4.675668002946606E-5	0.0	4.519812402848386E-4	5.454946003437707E-5
31	0.0010052686206335202	4.675668002946606E-5	7.79278000491101E-6	4.519812402848386E-4	5.454946003437707E-5
32	0.0010598180806678974	4.675668002946606E-5	7.79278000491101E-6	4.519812402848386E-4	6.234224003928808E-5
33	0.0011845025607464736	4.675668002946606E-5	7.79278000491101E-6	4.519812402848386E-4	6.234224003928808E-5
34	0.0012234664607710286	4.675668002946606E-5	7.79278000491101E-6	4.519812402848386E-4	7.01350200441991E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGA	62000	0.0	39.590324	12
CAACGAA	62655	0.0	39.41904	13
AACGAAG	64270	0.0	39.116226	14
CGAAGAG	64385	0.0	39.083637	16
AAGCAGT	216840	0.0	38.622944	1
ACGAAGA	65235	0.0	38.592777	15
AGCAGTG	222035	0.0	37.69856	2
GAAGAGT	66945	0.0	37.541264	17
AAGAGTA	66650	0.0	37.51838	18
GCAGTGG	225245	0.0	37.1915	3
AGTGGTA	224865	0.0	37.17075	5
ATCAACG	225290	0.0	37.08376	11
GTGGTAT	225075	0.0	37.060535	6
CAGTGGT	225915	0.0	36.98559	4
TGGTATC	224860	0.0	36.91986	7
GGTATCA	225150	0.0	36.889187	8
AGAGTAC	225975	0.0	36.867794	19
GTATCAA	225750	0.0	36.857586	9
GAGTACT	68505	0.0	36.771038	20
TATCAAC	226605	0.0	36.73882	10
>>END_MODULE