FastQCFastQC Report
Thu 26 May 2016
SRR805384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12147248
Sequences flagged as poor quality0
Sequence length46
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT704980.5803619058407303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGA116500.037.4592312
CAACGAA120200.036.6555713
AACGAAG125150.035.94087214
CGAAGAG125500.035.84063716
ACGAAGA132650.033.93893415
AAGCAGT472600.032.5179861
AGCAGTG518300.029.9826352
GAAGAGT156400.028.88746817
AAGAGTA155050.028.88100618
ATCAACG543450.028.48836111
AGTGGTA550750.028.1180215
GCAGTGG553850.028.0798033
AGAGTAC556250.027.77887719
GTGGTAT556750.027.771896
GGTATCA555800.027.6754238
CAGTGGT563350.027.5139774
TGGTATC560500.027.4469227
GTATCAA562450.027.4051029
TATCAAC567400.027.22241610
GTACATG408450.026.40714822
Produced by FastQC (version 0.11.5)