FastQCFastQC Report
Thu 26 May 2016
SRR805383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11438120
Sequences flagged as poor quality0
Sequence length46
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT4264473.728296258476043No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC490350.4286980727602088No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC317060.27719590282319123No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG283310.24768930558518357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGA753750.039.70016512
CAACGAA758700.039.5228713
CGAAGAG775000.039.41935316
AACGAAG775400.039.3835414
AAGCAGT2604050.039.167451
ACGAAGA780600.039.1442515
AGCAGTG2646250.038.5050542
GAAGAGT793550.038.43992217
AAGAGTA795750.038.1778218
GCAGTGG2676450.038.0959853
AGTGGTA2683750.037.9325
ATCAACG2685800.037.8732611
GTGGTAT2689950.037.8415956
CAGTGGT2693450.037.7842564
TGGTATC2680800.037.7611167
GGTATCA2685000.037.7407848
GAGTACT759650.037.72263720
AGAGTAC2688850.037.71352419
GTATCAA2692550.037.6847239
TATCAAC2695500.037.65015810