##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805383_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11438120
Sequences flagged as poor quality	0
Sequence length	46
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.8879715372806	37.0	35.0	37.0	35.0	37.0
2	35.95083755022679	37.0	36.0	37.0	35.0	37.0
3	37.83296118592916	39.0	39.0	39.0	35.0	39.0
4	37.8518577353621	39.0	38.0	39.0	35.0	39.0
5	37.87880237311726	39.0	38.0	39.0	35.0	39.0
6	37.854183554640095	39.0	38.0	39.0	35.0	39.0
7	37.77292422181267	39.0	38.0	39.0	35.0	39.0
8	38.68933635947166	40.0	39.0	40.0	36.0	40.0
9	38.767884145296605	40.0	39.0	40.0	36.0	40.0
10	38.51939733102992	40.0	39.0	40.0	36.0	40.0
11	38.74086440778729	40.0	39.0	40.0	36.0	40.0
12	38.80938056254	40.0	39.0	40.0	36.0	40.0
13	38.76137783132193	40.0	39.0	40.0	36.0	40.0
14	38.8211748958745	40.0	39.0	40.0	36.0	40.0
15	38.83306111493847	40.0	40.0	40.0	36.0	40.0
16	38.87914054057835	40.0	40.0	40.0	36.0	40.0
17	38.89148881109833	40.0	40.0	40.0	37.0	40.0
18	26.79736451444818	39.0	3.0	40.0	3.0	40.0
19	31.258451651145467	39.0	18.0	40.0	17.0	40.0
20	35.770380796844236	39.0	32.0	40.0	30.0	40.0
21	37.51001563193952	39.0	37.0	40.0	34.0	40.0
22	38.16295011767668	39.0	38.0	40.0	35.0	40.0
23	37.90976025780461	40.0	38.0	40.0	34.0	40.0
24	38.20373243155344	40.0	38.0	40.0	35.0	40.0
25	38.402992362381234	40.0	39.0	40.0	35.0	40.0
26	38.35478251670729	40.0	39.0	40.0	35.0	40.0
27	38.399789038758115	40.0	39.0	40.0	35.0	40.0
28	38.18761028910345	40.0	38.0	40.0	35.0	40.0
29	38.216453140900775	40.0	38.0	40.0	35.0	40.0
30	38.2215737376422	40.0	38.0	40.0	35.0	40.0
31	38.03181519340591	40.0	38.0	40.0	34.0	40.0
32	37.90503806569611	40.0	38.0	40.0	34.0	40.0
33	37.589272450367716	40.0	38.0	40.0	33.0	40.0
34	37.4196914353058	40.0	38.0	40.0	33.0	40.0
35	25.67070244061087	37.0	3.0	40.0	3.0	40.0
36	29.502729731809072	37.0	15.0	40.0	15.0	40.0
37	28.919289533594682	37.0	23.0	40.0	3.0	40.0
38	32.03204687483608	37.0	26.0	40.0	15.0	40.0
39	34.29769787342675	38.0	30.0	40.0	22.0	40.0
40	25.315374554559664	36.0	3.0	40.0	3.0	40.0
41	29.05560747745259	35.0	15.0	40.0	12.0	40.0
42	32.58855362594552	35.0	27.0	40.0	22.0	40.0
43	29.483360727112498	37.0	25.0	40.0	3.0	40.0
44	31.977138550740857	38.0	26.0	40.0	12.0	40.0
45	34.30646364962074	38.0	30.0	40.0	22.0	40.0
46	35.471908582879	38.0	34.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	10.0
11	47.0
12	83.0
13	185.0
14	378.0
15	703.0
16	1309.0
17	2183.0
18	3385.0
19	5138.0
20	7990.0
21	12332.0
22	18896.0
23	28808.0
24	43717.0
25	63467.0
26	89549.0
27	128713.0
28	186386.0
29	296068.0
30	652086.0
31	1413528.0
32	475864.0
33	469618.0
34	854147.0
35	423348.0
36	322099.0
37	541337.0
38	1296308.0
39	4100433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.567570544809811	43.60597720604435	25.722846062115103	17.10360618703074
2	20.10882033061377	45.91890975090312	16.988543571845725	16.983726346637383
3	42.652901001213486	21.360651925316397	18.331710106206263	17.65473696726385
4	18.98204425202743	20.47887240210804	19.19443929596822	41.34464404989631
5	18.94525498945631	43.24890803733481	20.309666273828217	17.496170699380666
6	41.848564274548615	20.638400366493794	19.40597755575217	18.10705780320542
7	19.08379174199956	20.132556748836347	43.28996373529916	17.493687773864934
8	41.11803338310842	21.13041303990516	20.34728609246974	17.404267484516687
9	40.62206026864555	21.080535962203577	20.731483845247293	17.56591992390358
10	17.79028371795365	21.157095746503796	43.305875441068984	17.746745094473567
11	17.639087542358357	43.567054725776615	20.92926110234899	17.86459662951604
12	17.682809762443476	21.249401125359764	43.169909040996245	17.89788007120051
13	17.898203550933196	21.211116861861914	20.74112703835945	40.14955254884544
14	17.880315995985356	43.651281854010975	20.544844782184484	17.923557367819186
15	17.886348455865125	43.427652446381046	20.541854780331033	18.144144317422793
16	17.940159746531773	20.937785230439967	20.65330666228366	40.4687483607446
17	40.66455851136376	20.70377824327774	20.732331886708653	17.899331358649846
18	15.660431959098172	42.95880791598619	14.56700052106465	26.813759603850983
19	18.139222179868717	43.511346270191254	20.621684332739996	17.72774721720003
20	40.731754868807116	20.93288932097233	20.80134672481142	17.534009085409142
21	18.1593915783363	43.29839169373988	20.862300797683535	17.679915930240284
22	40.387537462450126	20.816768839634488	21.027161806310826	17.768531891604564
23	18.058763153385346	20.914914339069707	43.23146635985634	17.794856147688606
24	17.93940787472067	43.14431042863687	20.837287945921183	18.078993750721274
25	17.942467818137946	20.958103254730673	20.99228719404937	40.10714173308201
26	18.363157581840373	36.61394529870293	27.279185740313967	17.743711379142727
27	18.613583351110147	20.596890048364592	43.16915716918515	17.620369431340116
28	34.797597856990485	20.474719621756023	27.139197700321382	17.58848482093211
29	34.58488807601249	20.6173654411739	27.100100366144087	17.69764611666952
30	34.23831014187646	20.65256353316804	27.17966763768871	17.929458687266788
31	27.403375729577938	23.053115372106607	28.929290827513615	20.614218070801847
32	24.555958496676027	25.869006445115104	29.99118736295825	19.58384769525062
33	24.377983444831845	24.924664193066693	30.12740730119985	20.569945060901617
34	23.13411644570961	24.074270946624097	31.00528758222505	21.786325025441243
35	16.314420551629112	48.05022153990341	21.316982161404148	14.318375747063328
36	22.225461876602097	25.179365140425176	31.30368452158222	21.29148846139051
37	18.437750259658056	40.402347588589734	24.768152458620822	16.391749693131388
38	23.22323074071613	24.639215185712338	31.42004105569797	20.717513017873568
39	23.328090630278403	24.64400618283424	31.05078456949219	20.977118617395167
40	14.32579829552409	51.727206918619494	19.954240731868524	13.992754053987893
41	22.593441929268096	25.971182327165653	30.53580483506031	20.899570908505943
42	22.87488678209356	26.046797900354253	30.627760506097157	20.450554811455028
43	17.491143649480858	40.737210310785336	25.39801121163268	16.37363482810112
44	22.453523830839334	24.766430147611672	31.377332988288288	21.40271303326071
45	22.88054330606778	24.339935234111902	31.035825817529457	21.743695642290866
46	23.02853965511815	24.422405080555194	30.546873087535364	22.00218217679129
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3033.0
1	2117.5
2	1202.0
3	1058.5
4	915.0
5	1019.5
6	1124.0
7	1124.0
8	1471.0
9	1818.0
10	2557.0
11	3296.0
12	5041.5
13	6787.0
14	10317.0
15	13847.0
16	19585.5
17	25324.0
18	35309.0
19	45294.0
20	45294.0
21	60152.0
22	75010.0
23	183205.5
24	291401.0
25	473750.5
26	656100.0
27	448608.5
28	241117.0
29	253093.5
30	265070.0
31	265070.0
32	298413.5
33	331757.0
34	373857.0
35	415957.0
36	468216.5
37	520476.0
38	580515.5
39	640555.0
40	714402.5
41	788250.0
42	850974.5
43	913699.0
44	913699.0
45	967153.5
46	1020608.0
47	1026000.5
48	1031393.0
49	1006081.5
50	980770.0
51	922244.5
52	863719.0
53	781580.0
54	699441.0
55	652686.0
56	605931.0
57	605931.0
58	484796.5
59	363662.0
60	302784.5
61	241907.0
62	201682.0
63	161457.0
64	132479.0
65	103501.0
66	84510.5
67	65520.0
68	49213.0
69	32906.0
70	32906.0
71	23652.0
72	14398.0
73	9942.0
74	5486.0
75	3981.5
76	2477.0
77	1863.5
78	1250.0
79	1001.0
80	752.0
81	752.0
82	602.5
83	453.0
84	344.5
85	236.0
86	192.0
87	148.0
88	97.0
89	46.0
90	29.5
91	13.0
92	11.0
93	9.0
94	9.0
95	6.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
46	1.143812E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.16437334993776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.13605109952616	50.238000949409845
2	10.375830568470285	12.692623494112269
3	3.4544236486138304	6.3386297325801175
4	1.4743838361954227	3.607190536726813
5	0.760635586156818	2.326189948747219
6	0.42088805128788115	1.5446012344499829
7	0.24766967447251187	1.0603992305825987
8	0.17223517446785047	0.842772521211462
9	0.12746724880260035	0.7016808956086491
>10	0.7317320046809448	8.342499682132624
>50	0.06383368530830616	2.6979517676715634
>100	0.03369514619128977	3.5995084927267635
>500	6.993837036401907E-4	0.26600403738870815
>1k	3.184246879703188E-4	0.44106232208963525
>5k	6.0652193059419276E-5	0.23939038566165752
>10k+	7.581524132427409E-5	5.061494768900109
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	426447	3.728296258476043	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGC	49035	0.4286980727602088	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	31706	0.27719590282319123	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGG	28331	0.24768930558518357	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0011103223256968802	0.0	0.0	0.0	0.0
2	0.0019321357006221302	0.0	0.0	0.0	0.0
3	0.0019408783961000584	0.0	0.0	0.0	0.0
4	0.0019408783961000584	0.0	0.0	0.0	0.0
5	0.0019408783961000584	0.0	0.0	0.0	0.0
6	0.002010819959923484	0.0	0.0	0.0	0.0
7	0.002010819959923484	0.0	0.0	1.7485390955856382E-5	0.0
8	0.0020195626554014123	8.742695477928191E-6	0.0	1.7485390955856382E-5	0.0
9	0.00202830535087934	8.742695477928191E-6	0.0	6.994156382342553E-5	0.0
10	0.002063276132791053	8.742695477928191E-6	0.0	6.994156382342553E-5	0.0
11	0.0020807615237469095	8.742695477928191E-6	0.0	6.994156382342553E-5	0.0
12	0.0020807615237469095	8.742695477928191E-6	0.0	7.868425930135373E-5	0.0
13	0.0020895042192248378	8.742695477928191E-6	0.0	7.868425930135373E-5	0.0
14	0.002115732305658622	1.7485390955856382E-5	0.0	7.868425930135373E-5	0.0
15	0.002115732305658622	1.7485390955856382E-5	0.0	7.868425930135373E-5	0.0
16	0.0021244750011365503	1.7485390955856382E-5	0.0	7.868425930135373E-5	0.0
17	0.0021332176966144785	1.7485390955856382E-5	0.0	7.868425930135373E-5	0.0
18	0.002150703087570335	1.7485390955856382E-5	0.0	7.868425930135373E-5	0.0
19	0.0021594457830482632	1.7485390955856382E-5	0.0	7.868425930135373E-5	0.0
20	0.002185673869482048	1.7485390955856382E-5	0.0	7.868425930135373E-5	0.0
21	0.0022293873468716887	1.7485390955856382E-5	0.0	8.742695477928191E-5	8.742695477928191E-6
22	0.0022643581287834016	1.7485390955856382E-5	0.0	8.742695477928191E-5	8.742695477928191E-6
23	0.002299328910695114	1.7485390955856382E-5	0.0	8.742695477928191E-5	8.742695477928191E-6
24	0.002334299692606827	1.7485390955856382E-5	0.0	1.3114043216892288E-4	8.742695477928191E-6
25	0.0023605277790406114	1.7485390955856382E-5	0.0	1.4862582312477924E-4	8.742695477928191E-6
26	0.0024479547338198933	1.7485390955856382E-5	0.0	3.234797326833431E-4	2.6228086433784572E-5
27	0.0025441243840771035	2.6228086433784572E-5	0.0	3.234797326833431E-4	2.6228086433784572E-5
28	0.00256160977503296	2.6228086433784572E-5	0.0	3.234797326833431E-4	2.6228086433784572E-5
29	0.0025878378614667447	2.6228086433784572E-5	0.0	3.234797326833431E-4	2.6228086433784572E-5
30	0.0026665221207680984	2.6228086433784572E-5	0.0	3.234797326833431E-4	2.6228086433784572E-5
31	0.0027189782936356674	2.6228086433784572E-5	0.0	3.234797326833431E-4	2.6228086433784572E-5
32	0.0028064052484149493	2.6228086433784572E-5	0.0	3.234797326833431E-4	2.6228086433784572E-5
33	0.002937545680583872	2.6228086433784572E-5	0.0	3.234797326833431E-4	2.6228086433784572E-5
34	0.003033715330841082	2.6228086433784572E-5	0.0	3.234797326833431E-4	2.6228086433784572E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGA	75375	0.0	39.700165	12
CAACGAA	75870	0.0	39.52287	13
CGAAGAG	77500	0.0	39.419353	16
AACGAAG	77540	0.0	39.38354	14
AAGCAGT	260405	0.0	39.16745	1
ACGAAGA	78060	0.0	39.14425	15
AGCAGTG	264625	0.0	38.505054	2
GAAGAGT	79355	0.0	38.439922	17
AAGAGTA	79575	0.0	38.17782	18
GCAGTGG	267645	0.0	38.095985	3
AGTGGTA	268375	0.0	37.932	5
ATCAACG	268580	0.0	37.87326	11
GTGGTAT	268995	0.0	37.841595	6
CAGTGGT	269345	0.0	37.784256	4
TGGTATC	268080	0.0	37.761116	7
GGTATCA	268500	0.0	37.740784	8
GAGTACT	75965	0.0	37.722637	20
AGAGTAC	268885	0.0	37.713524	19
GTATCAA	269255	0.0	37.684723	9
TATCAAC	269550	0.0	37.650158	10
>>END_MODULE