FastQCFastQC Report
Thu 26 May 2016
SRR805382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20043158
Sequences flagged as poor quality0
Sequence length53
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750450.8733404187104646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1055600.043.0551341
ATCAACG1084550.041.8646911
TCAACGC1064800.041.7826812
CAACGCA1066950.041.70949613
GGTATCA1088500.041.6544768
GTATCAA1091250.041.5473489
AGTGGTA1091200.041.499725
AACGCAG1076750.041.314614
GTGGTAT1097100.041.2936826
TATCAAC1107800.040.99453410
TGGTATC1107050.040.9437687
CGCAGAG1095400.040.58759316
GCAGTGG1117600.040.5446433
ACGCAGA1099150.040.4662215
AGAGTAC1108850.040.38986219
CAGTGGT1128100.040.0922784
GAGTACT470150.039.99223320
AGCAGTG1143450.039.7904132
GTACTTT463250.039.7001622
CAGAGTA1121000.039.31480818
Produced by FastQC (version 0.11.5)