##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805382_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20043158
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.203058021096275	37.0	37.0	37.0	35.0	37.0
2	36.237387491532026	37.0	37.0	37.0	35.0	37.0
3	37.940714781572844	39.0	38.0	39.0	35.0	39.0
4	37.793861376535574	39.0	38.0	39.0	35.0	39.0
5	37.72056678892618	39.0	38.0	39.0	35.0	39.0
6	37.61972205178446	39.0	37.0	39.0	35.0	39.0
7	37.535799797616725	39.0	37.0	39.0	35.0	39.0
8	38.26431608232595	40.0	38.0	40.0	35.0	40.0
9	38.09151811306382	40.0	38.0	40.0	35.0	40.0
10	37.96910177527913	39.0	38.0	40.0	35.0	40.0
11	37.28451689100091	38.0	36.0	40.0	33.0	40.0
12	37.1576855802863	38.0	36.0	40.0	33.0	40.0
13	36.9268170215492	38.0	36.0	40.0	33.0	40.0
14	36.47614347998454	38.0	35.0	39.0	32.0	40.0
15	36.384188858861464	38.0	35.0	39.0	32.0	40.0
16	35.851078208334236	37.0	34.0	39.0	31.0	40.0
17	34.98588301304615	36.0	33.0	38.0	29.0	39.0
18	34.00275605271385	36.0	32.0	38.0	27.0	39.0
19	34.3500757714927	36.0	32.0	38.0	27.0	39.0
20	33.48827994071593	35.0	31.0	38.0	27.0	39.0
21	33.13471145614878	34.0	31.0	37.0	27.0	39.0
22	32.77170154523554	34.0	30.0	37.0	26.0	39.0
23	32.59299502603332	34.0	30.0	36.0	27.0	38.0
24	31.006634333771157	32.0	29.0	36.0	24.0	38.0
25	30.448044464849303	32.0	27.0	36.0	19.0	38.0
26	30.376740880853205	32.0	27.0	35.0	22.0	37.0
27	29.895990342440047	31.0	27.0	34.0	24.0	36.0
28	28.953979707189855	31.0	25.0	34.0	18.0	37.0
29	28.332843107857553	30.0	25.0	34.0	17.0	36.0
30	27.828358235763048	30.0	25.0	33.0	16.0	35.0
31	27.4963809096351	29.0	24.0	33.0	16.0	35.0
32	26.90145569874767	29.0	24.0	31.0	16.0	35.0
33	26.252525225815212	27.0	24.0	31.0	16.0	34.0
34	25.60026149571839	27.0	21.0	31.0	15.0	34.0
35	24.93687845997123	26.0	19.0	30.0	14.0	33.0
36	24.226394463387457	26.0	19.0	30.0	10.0	33.0
37	23.562807318088296	25.0	18.0	30.0	10.0	33.0
38	22.85365190455516	24.0	16.0	30.0	10.0	32.0
39	22.20803243680462	24.0	15.0	30.0	10.0	31.0
40	21.498837109401624	24.0	15.0	28.0	10.0	30.0
41	20.916533911472435	23.0	15.0	27.0	10.0	30.0
42	20.391795993425788	22.0	14.0	27.0	10.0	30.0
43	19.794770913845014	20.0	12.0	26.0	9.0	30.0
44	19.15971559970739	19.0	10.0	25.0	9.0	30.0
45	18.515134990204636	19.0	10.0	24.0	9.0	30.0
46	18.028522002371083	18.0	10.0	24.0	9.0	28.0
47	17.436419450467834	16.0	10.0	24.0	9.0	28.0
48	16.974071600892433	15.0	10.0	23.0	9.0	27.0
49	16.51067172149219	15.0	10.0	23.0	9.0	26.0
50	16.006278551513688	14.0	9.0	23.0	9.0	26.0
51	15.55152386664816	14.0	9.0	22.0	9.0	24.0
52	15.103517369867562	13.0	9.0	22.0	8.0	24.0
53	14.028759839143113	13.0	9.0	19.0	8.0	23.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	25.0
12	194.0
13	1008.0
14	2634.0
15	4067.0
16	4861.0
17	6932.0
18	14580.0
19	32653.0
20	67553.0
21	122561.0
22	201247.0
23	305641.0
24	451818.0
25	691534.0
26	1250599.0
27	2897204.0
28	5564668.0
29	5150957.0
30	2094162.0
31	557647.0
32	180697.0
33	106410.0
34	92832.0
35	101844.0
36	93704.0
37	44578.0
38	544.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.233130028711043	37.96840298320255	27.693964194664332	23.104502793422075
2	28.002483440982708	43.754217773466635	12.67179553242059	15.57150325313007
3	30.771368463991557	28.95966294333458	19.810720446348824	20.458248146325044
4	21.76380588328446	26.48334159716747	21.787849000641515	29.965003518906553
5	19.850813928623424	31.795293935217195	26.141199904725593	22.21269223143379
6	26.27065056314978	25.962335875414443	24.550871674014644	23.216141887421134
7	22.36329225165016	25.449602303189945	29.91176340574674	22.27534203941315
8	25.381404467300012	27.499179520512683	26.13938382364695	20.98003218854035
9	26.181552827154285	26.789091818764287	26.117126851966145	20.912228502115283
10	21.075396402103898	27.402034150506623	30.582136806984213	20.94043264040527
11	20.841975101927552	32.15904399895466	26.104309510507278	20.894671388610515
12	20.719768810882996	26.874218124708694	30.842759409470304	21.56325365493801
13	20.85797058527404	27.157781223896954	26.185080215403183	25.79916797542583
14	21.346950415697965	31.99350122370936	25.739097601286186	20.92045075930649
15	21.029196097740684	31.661193310954296	25.796254262926034	21.51335632837899
16	21.66504899078279	26.64021308418564	25.93024512404682	25.764492800984755
17	26.389489121424877	26.435893984371127	25.89411808258958	21.280498811614418
18	21.250633258491504	26.717935367270968	25.77480055787616	26.256630816361376
19	21.656776841254256	31.339317885933944	25.80935599070765	21.19454928210415
20	26.388755704066195	26.955597516119965	25.735091246598962	20.920555533214877
21	21.57130128894858	31.259006190541434	25.94205962952545	21.227632890984545
22	25.80646722437652	26.396229576197523	26.44381688753838	21.35348631188758
23	21.249770121055775	26.773645151128378	30.9066415581816	21.069943169634247
24	21.44206516757489	30.87908103104311	26.105357249591105	21.573496551790893
25	21.154929777034138	26.70155072369334	26.31656648119024	25.826953018082282
26	21.547103505345813	29.127615518472687	28.20823944011218	21.117041536069316
27	21.615730415336746	26.362916462565426	30.839686041491067	21.181667080606758
28	23.986145297063466	26.400166081612486	28.31390143209967	21.29978718922437
29	24.32448519340116	26.460615637515804	28.08615787991094	21.128741289172094
30	24.483058009122118	26.26004345223442	28.00506287482242	21.25183566382104
31	22.97133016663342	26.806489276789613	28.461398148934414	21.760782407642548
32	22.434763024868637	27.42101818485889	28.643101052239373	21.5011177380331
33	22.29037460064926	26.990088088912934	28.827178830801014	21.89235847963679
34	21.991858767964608	27.1490301079301	29.00327383539061	21.85583728871468
35	21.843573752200125	27.22793483941004	29.052008670489947	21.876482737899885
36	21.831175506374795	27.113531709923155	29.150101994905192	21.905190788796855
37	21.901099617136182	27.257491060041538	29.2236333216552	21.617776001167083
38	21.963305383313347	27.07600768302081	29.38201155726059	21.578675376405254
39	21.825487779919712	26.65097985058043	29.63865774046186	21.884874629038
40	21.589412207397658	26.848274109299542	29.75235738799245	21.80995629531035
41	21.629016744766467	26.971104054560662	29.70377721913882	21.696101981534046
42	21.63407582777125	26.744742520115842	29.880440996373924	21.74074065573898
43	21.461368512886043	26.777701398152924	30.047939551242376	21.712990537718657
44	21.477927779644308	26.665947551778018	30.11251520344249	21.743609465135187
45	21.36899285032828	26.478127847916983	30.248960767559684	21.90391853419506
46	21.257074359240196	26.495445478202583	30.227147837681066	22.02033232487615
47	21.473956349593212	26.46242672936071	30.163390419813084	21.90022650123299
48	21.306851944189635	26.50494497922932	30.205085446115827	21.98311763046522
49	21.218337948540842	26.48347630647825	30.238448452085244	22.059737292895658
50	21.430355436004646	26.567539905637627	29.95861231049518	22.043492347862546
51	21.29214368314614	26.485292387556893	29.914692085947735	22.307871843349236
52	21.21072936709874	26.651154473761068	29.82692148612509	22.311194673015102
53	20.895479644475188	27.12443318562873	29.77297290177526	22.207114268120822
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	118.0
1	122.0
2	126.0
3	161.0
4	196.0
5	240.5
6	285.0
7	411.0
8	827.0
9	1117.0
10	1761.0
11	2405.0
12	3883.0
13	5361.0
14	8247.5
15	11134.0
16	17598.5
17	24063.0
18	35090.0
19	46117.0
20	68429.0
21	90741.0
22	227868.0
23	364995.0
24	331497.0
25	311175.0
26	324351.0
27	361817.5
28	399284.0
29	454018.0
30	508752.0
31	568456.0
32	628160.0
33	693939.5
34	759719.0
35	836042.5
36	912366.0
37	993622.5
38	1074879.0
39	1158196.0
40	1241513.0
41	1317852.0
42	1443560.5
43	1492930.0
44	1521863.0
45	1550796.0
46	1541525.5
47	1532255.0
48	1508366.0
49	1484477.0
50	1423761.0
51	1363045.0
52	1279498.0
53	1195951.0
54	1097765.5
55	999580.0
56	889552.5
57	779525.0
58	673439.0
59	478250.5
60	389148.0
61	322618.5
62	256089.0
63	207517.5
64	158946.0
65	124623.5
66	90301.0
67	70304.5
68	50308.0
69	37385.0
70	24462.0
71	17661.0
72	10860.0
73	7891.5
74	4923.0
75	3475.5
76	1499.5
77	971.0
78	699.5
79	428.0
80	330.0
81	232.0
82	161.0
83	90.0
84	57.0
85	24.0
86	21.5
87	19.0
88	13.0
89	7.0
90	4.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.0043158E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.88758411710991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43724591468538	52.02828898640227
2	11.185860985408429	14.292752692551577
3	3.3097105909294906	6.343482413438921
4	1.4343180833038018	3.66540468791054
5	0.7363802592022554	2.352277787598164
6	0.4279314702682816	1.6403704681874014
7	0.29359071654457675	1.3129761119470953
8	0.19249954141746176	0.9838664515850536
9	0.14375805578642614	0.8265919393410731
>10	0.7465101379226767	8.83816055216621
>50	0.06138188877736322	2.708480171588259
>100	0.02964260164407202	3.208595392681133
>500	8.640465966701473E-4	0.35920484682747883
>1k	2.4308053844544715E-4	0.24769396638197805
>5k	3.13134872710673E-5	0.12278332981836163
>10k+	3.131348727106306E-5	1.0690702015743605
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	175045	0.8733404187104646	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9935402395171458E-5	4.989233732528577E-6	0.0	0.0	0.0
2	3.9913869860228615E-5	4.989233732528577E-6	0.0	0.0	0.0
3	3.9913869860228615E-5	4.989233732528577E-6	0.0	0.0	0.0
4	3.9913869860228615E-5	4.989233732528577E-6	0.0	0.0	0.0
5	3.9913869860228615E-5	4.989233732528577E-6	0.0	0.0	4.989233732528577E-6
6	3.9913869860228615E-5	4.989233732528577E-6	0.0	4.989233732528577E-6	4.989233732528577E-6
7	3.9913869860228615E-5	4.989233732528577E-6	0.0	4.989233732528577E-6	4.989233732528577E-6
8	3.9913869860228615E-5	4.989233732528577E-6	0.0	9.978467465057154E-6	4.989233732528577E-6
9	3.9913869860228615E-5	4.989233732528577E-6	4.989233732528577E-6	1.9956934930114308E-5	4.989233732528577E-6
10	3.9913869860228615E-5	4.989233732528577E-6	4.989233732528577E-6	1.9956934930114308E-5	4.989233732528577E-6
11	4.490310359275719E-5	1.4967701197585729E-5	4.989233732528577E-6	1.9956934930114308E-5	4.989233732528577E-6
12	5.488157105781434E-5	1.4967701197585729E-5	4.989233732528577E-6	2.9935402395171458E-5	9.978467465057154E-6
13	6.48600385228715E-5	1.9956934930114308E-5	4.989233732528577E-6	2.9935402395171458E-5	9.978467465057154E-6
14	7.982773972045723E-5	1.9956934930114308E-5	4.989233732528577E-6	3.4924636127700035E-5	9.978467465057154E-6
15	1.0976314211562869E-4	1.9956934930114308E-5	4.989233732528577E-6	3.4924636127700035E-5	9.978467465057154E-6
16	1.29720077045743E-4	1.9956934930114308E-5	4.989233732528577E-6	3.9913869860228615E-5	1.4967701197585729E-5
17	1.7961241437102876E-4	2.4946168662642885E-5	4.989233732528577E-6	3.9913869860228615E-5	1.4967701197585729E-5
18	1.9956934930114308E-4	2.9935402395171458E-5	4.989233732528577E-6	3.9913869860228615E-5	1.4967701197585729E-5
19	2.59440154091486E-4	2.9935402395171458E-5	4.989233732528577E-6	3.9913869860228615E-5	1.4967701197585729E-5
20	2.8438632275412884E-4	3.4924636127700035E-5	4.989233732528577E-6	3.9913869860228615E-5	2.4946168662642885E-5
21	3.3427866007941466E-4	4.989233732528577E-5	4.989233732528577E-6	3.9913869860228615E-5	2.4946168662642885E-5
22	3.791817636721718E-4	5.488157105781434E-5	4.989233732528577E-6	3.9913869860228615E-5	2.4946168662642885E-5
23	4.2907410099745757E-4	5.488157105781434E-5	9.978467465057154E-6	4.490310359275719E-5	3.4924636127700035E-5
24	5.18880308182972E-4	5.488157105781434E-5	9.978467465057154E-6	5.488157105781434E-5	4.490310359275719E-5
25	5.787511129733149E-4	5.488157105781434E-5	9.978467465057154E-6	5.488157105781434E-5	4.490310359275719E-5
26	6.186649828335435E-4	5.488157105781434E-5	9.978467465057154E-6	5.488157105781434E-5	4.490310359275719E-5
27	7.334173586817008E-4	5.9870804790342916E-5	9.978467465057154E-6	5.488157105781434E-5	4.490310359275719E-5
28	8.581482019949151E-4	5.9870804790342916E-5	9.978467465057154E-6	5.9870804790342916E-5	4.490310359275719E-5
29	9.77889811575601E-4	5.9870804790342916E-5	9.978467465057154E-6	7.483850598792864E-5	4.490310359275719E-5
30	0.0011026206548888154	5.9870804790342916E-5	9.978467465057154E-6	7.483850598792864E-5	4.989233732528577E-5
31	0.0012672653680622585	5.9870804790342916E-5	9.978467465057154E-6	7.982773972045723E-5	4.989233732528577E-5
32	0.00143689931496823	5.9870804790342916E-5	9.978467465057154E-6	9.479544091804295E-5	4.989233732528577E-5
33	0.001721285637722359	6.48600385228715E-5	9.978467465057154E-6	1.1475237584815726E-4	4.989233732528577E-5
34	0.0018360380135705161	6.48600385228715E-5	9.978467465057154E-6	1.1475237584815726E-4	4.989233732528577E-5
35	0.001980725791813845	6.984927225540007E-5	9.978467465057154E-6	1.29720077045743E-4	4.989233732528577E-5
36	0.0021553489724523453	6.984927225540007E-5	9.978467465057154E-6	1.3969854451080014E-4	4.989233732528577E-5
37	0.0023349613868233737	6.984927225540007E-5	9.978467465057154E-6	1.6464471317344302E-4	4.989233732528577E-5
38	0.0025045953337293455	6.984927225540007E-5	9.978467465057154E-6	2.344939854288431E-4	5.488157105781434E-5
39	0.0027091539167630172	6.984927225540007E-5	9.978467465057154E-6	2.6941862155654316E-4	5.9870804790342916E-5
40	0.002983561772052089	6.984927225540007E-5	9.978467465057154E-6	2.8438632275412884E-4	5.9870804790342916E-5
41	0.0031931095888182892	6.984927225540007E-5	9.978467465057154E-6	3.442571275444718E-4	6.48600385228715E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	105560	0.0	43.055134	1
ATCAACG	108455	0.0	41.86469	11
TCAACGC	106480	0.0	41.78268	12
CAACGCA	106695	0.0	41.709496	13
GGTATCA	108850	0.0	41.654476	8
GTATCAA	109125	0.0	41.547348	9
AGTGGTA	109120	0.0	41.49972	5
AACGCAG	107675	0.0	41.3146	14
GTGGTAT	109710	0.0	41.293682	6
TATCAAC	110780	0.0	40.994534	10
TGGTATC	110705	0.0	40.943768	7
CGCAGAG	109540	0.0	40.587593	16
GCAGTGG	111760	0.0	40.544643	3
ACGCAGA	109915	0.0	40.46622	15
AGAGTAC	110885	0.0	40.389862	19
CAGTGGT	112810	0.0	40.092278	4
GAGTACT	47015	0.0	39.992233	20
AGCAGTG	114345	0.0	39.790413	2
GTACTTT	46325	0.0	39.70016	22
CAGAGTA	112100	0.0	39.314808	18
>>END_MODULE