FastQCFastQC Report
Thu 26 May 2016
SRR805381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15230149
Sequences flagged as poor quality0
Sequence length53
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT995950.6539331952694619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT588900.041.4971161
ATCAACG620300.039.21465311
TCAACGC607600.039.18342612
CAACGCA608150.039.1634513
GGTATCA623250.038.9310078
GTATCAA624750.038.8450559
AGTGGTA625700.038.7597855
AACGCAG617550.038.54829814
GTGGTAT629150.038.5323076
TATCAAC637800.038.18657710
TGGTATC635900.038.1306847
ACGCAGA629650.037.82617615
CGCAGAG630000.037.76785716
AGAGTAC637800.037.47177519
CAGTGGT650700.037.263414
GCAGTGG653000.037.1033673
GAGTACT281750.037.0244920
CAGAGTA647000.036.3469118
AGCAGTG676250.036.0223272
GTACTTT284700.035.82367322
Produced by FastQC (version 0.11.5)