##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805381_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15230149
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.26361363897359	35.0	35.0	35.0	33.0	36.0
2	35.20399314543804	35.0	35.0	37.0	35.0	37.0
3	37.734128930714995	39.0	37.0	39.0	35.0	39.0
4	37.713579427226875	39.0	37.0	39.0	35.0	39.0
5	37.734631880489154	39.0	38.0	39.0	35.0	39.0
6	37.67667433851107	39.0	37.0	39.0	35.0	39.0
7	37.626844294169416	39.0	37.0	39.0	35.0	39.0
8	38.34840893546084	40.0	38.0	40.0	36.0	40.0
9	38.17655592207272	40.0	38.0	40.0	35.0	40.0
10	38.057259715581246	39.0	38.0	40.0	35.0	40.0
11	37.40955416785483	38.0	36.0	40.0	33.0	40.0
12	37.28676423323239	38.0	36.0	40.0	33.0	40.0
13	37.0667686179564	38.0	36.0	40.0	33.0	40.0
14	36.612777327391875	38.0	36.0	39.0	32.0	40.0
15	36.514933964204815	38.0	36.0	39.0	32.0	40.0
16	35.97755222224024	38.0	34.0	39.0	31.0	40.0
17	35.097850388725675	36.0	33.0	38.0	29.0	39.0
18	34.08706861633461	36.0	32.0	38.0	27.0	39.0
19	34.39720504375893	36.0	32.0	38.0	28.0	39.0
20	33.5904948139378	35.0	31.0	38.0	27.0	39.0
21	33.23642165286761	34.0	31.0	37.0	27.0	39.0
22	32.83465795377314	34.0	31.0	37.0	26.0	39.0
23	32.71311252437517	34.0	30.0	37.0	27.0	38.0
24	31.10744720882245	32.0	29.0	36.0	24.0	38.0
25	30.524107413525634	32.0	27.0	36.0	19.0	38.0
26	30.494979990018482	32.0	27.0	35.0	23.0	37.0
27	30.051046644389363	31.0	27.0	34.0	24.0	36.0
28	29.049765632627757	31.0	25.0	34.0	18.0	37.0
29	28.521198774877384	30.0	25.0	34.0	18.0	36.0
30	28.07153856472448	30.0	25.0	33.0	18.0	35.0
31	27.696613867664723	30.0	25.0	33.0	17.0	35.0
32	27.116201949173313	29.0	24.0	32.0	16.0	35.0
33	26.463984167193637	27.0	24.0	31.0	16.0	34.0
34	25.80562750896265	27.0	22.0	31.0	15.0	34.0
35	25.137148494082364	27.0	19.0	30.0	15.0	33.0
36	24.411300112690952	26.0	19.0	30.0	10.0	33.0
37	23.772151342708465	25.0	18.0	30.0	10.0	33.0
38	23.043169308455223	24.0	18.0	30.0	10.0	33.0
39	22.390726315284244	24.0	16.0	30.0	10.0	31.0
40	21.660767074570316	24.0	15.0	28.0	10.0	31.0
41	21.1161255218186	23.0	15.0	27.0	10.0	30.0
42	20.57020985152542	23.0	14.0	27.0	10.0	30.0
43	19.96693637074726	21.0	13.0	26.0	9.0	30.0
44	19.340508290496697	19.0	10.0	25.0	9.0	30.0
45	18.685663482346758	19.0	10.0	24.0	9.0	30.0
46	18.177295113790418	18.0	10.0	24.0	9.0	28.0
47	17.610112612818167	18.0	10.0	24.0	9.0	28.0
48	17.14647512640881	15.0	10.0	23.0	9.0	28.0
49	16.667032738812996	15.0	10.0	23.0	9.0	27.0
50	16.138044217426895	14.0	9.0	23.0	9.0	26.0
51	15.685321397709242	14.0	9.0	22.0	9.0	25.0
52	15.241697504075633	13.0	9.0	22.0	8.0	24.0
53	14.184729709472967	13.0	9.0	19.0	8.0	23.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	11.0
11	60.0
12	372.0
13	1369.0
14	3004.0
15	4434.0
16	5393.0
17	7562.0
18	13209.0
19	26298.0
20	50145.0
21	88190.0
22	142040.0
23	215141.0
24	319560.0
25	493917.0
26	898634.0
27	2071896.0
28	4075902.0
29	4062185.0
30	1804183.0
31	494026.0
32	157009.0
33	85547.0
34	66581.0
35	64401.0
36	54776.0
37	24179.0
38	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.624518512589733	48.66565652115419	17.91818320359177	22.791641762664305
2	27.82448812549372	40.91571920931305	19.099865667762018	12.159926997431214
3	29.417643911428577	26.587087230728994	23.944237183759657	20.05103167408277
4	20.914188035849158	28.75369111621955	23.28428960215688	27.047831245774418
5	19.570307552473714	32.40291345803642	27.275806691057326	20.750972298432536
6	24.29733287573221	25.869221634010277	26.673639240167645	23.159806250089872
7	22.802331086846227	26.790768757416622	28.967234660672066	21.439665495065086
8	23.139379660698	27.157849867391313	28.30439150660969	21.398378965300996
9	24.559897608355637	26.27870548081966	28.24103690646756	20.920360004357146
10	21.444038400412236	27.805913126654243	29.809734625708522	20.940313847225
11	21.023825833877265	30.814137143372662	26.996735225636993	21.16530179711308
12	21.104777110191108	26.945317475226275	29.968774435496332	21.981130979086284
13	21.314847280876897	27.859648648217426	26.385263860517718	24.440240210387962
14	21.504897949455387	31.040326657342614	26.260924958777487	21.193850434424508
15	21.348399152234162	30.62558350545356	26.153821607392025	21.872195734920254
16	22.147235723038563	27.05618310103204	26.333084462929417	24.463496712999984
17	25.230495118596675	26.762351438584087	26.309933015100505	21.69722042771873
18	21.59178482101521	27.12557835120326	25.99775616115115	25.28488066663038
19	22.16627033655416	30.35968328346624	25.99612781201287	21.477918567966736
20	25.252077310602804	27.51664478134784	25.997303112398967	21.23397479565039
21	22.24307194893497	30.095102812191794	26.134832955344034	21.526992283529204
22	24.819507675203965	26.951995019878005	26.71531315944447	21.51318414547356
23	21.707528928311863	27.15672052847283	29.866956652886323	21.26879389032898
24	21.900258493859777	29.77122548177303	26.273242632097688	22.055273392269502
25	21.47967166965996	27.280153332708696	26.472741665232558	24.767433332398785
26	21.889989388810314	28.559464520012245	28.19803667055391	21.352509420623527
27	22.07902890510132	26.542353590893956	29.79458047324422	21.5840370307605
28	23.63407606846131	26.69947615088992	28.0195354621941	21.646912318454664
29	23.832682135939706	26.621446710731455	28.002444362166123	21.543426791162712
30	24.067269466634897	26.467226289119033	27.83704873799987	21.628455506246198
31	22.98619008914489	27.044160894289348	28.037663978205334	21.931985038360427
32	22.465945671312866	27.420723198440143	28.458539703058715	21.654791427188268
33	22.500219794304048	26.977378881848104	28.433845263102807	22.08855606074504
34	22.186480250455855	27.48868051126749	28.384850338627675	21.93998889964898
35	22.022135174120752	27.324696560749338	28.723047949169768	21.93012031596014
36	22.184385720717508	27.078861802336927	28.673757558117124	22.062994918828437
37	22.18332204103847	27.381636253197524	28.632523555744594	21.802518150019413
38	22.241082473979738	27.06423948971215	29.053090682172577	21.641587354135538
39	22.157642712490862	26.661741785979903	29.102315413985774	22.078300087543465
40	21.91825569139212	26.970300815835746	29.094994408787468	22.016449083984668
41	21.857619383763087	26.8756201925536	29.39123576532311	21.875524658360206
42	21.984847292038967	26.638557508531267	29.393021696636062	21.983573502793703
43	21.696038561408688	26.97447674346456	29.43518149428479	21.89430320084196
44	21.652480221959745	26.629076314355167	29.858407819910365	21.860035643774726
45	21.722643685232494	26.481710717340977	29.658698677209266	22.136946920217262
46	21.616676238689458	26.693816324449614	29.57406391756246	22.115443519298463
47	21.70489599281005	26.487902383620803	29.776806517125998	22.030395106443148
48	21.54422126796002	26.52471751917857	29.69717499152503	22.233886221336377
49	21.505081795325836	26.596049716913473	29.648324517376683	22.250543970384008
50	21.615159510258238	26.609601783935272	29.611279574480854	22.163959131325637
51	21.6056586183103	26.328435788776588	29.50089326112305	22.565012331790058
52	21.52148347333962	26.703993506563855	29.315465003001613	22.459058017094907
53	21.05099562716031	27.086800004386035	29.52663168298616	22.33557268546749
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	127.0
1	148.0
2	169.0
3	216.5
4	264.0
5	334.0
6	404.0
7	530.0
8	874.5
9	1093.0
10	1796.0
11	2499.0
12	3869.0
13	5239.0
14	8060.0
15	10881.0
16	17070.5
17	23260.0
18	33591.0
19	43922.0
20	61992.0
21	80062.0
22	168386.5
23	256711.0
24	244254.0
25	245273.5
26	258750.0
27	290170.5
28	321591.0
29	363506.5
30	405422.0
31	450329.5
32	495237.0
33	545402.5
34	595568.0
35	651916.0
36	708264.0
37	764294.0
38	820324.0
39	881625.5
40	942927.0
41	999746.5
42	1091943.5
43	1127321.0
44	1143653.5
45	1159986.0
46	1148427.0
47	1136868.0
48	1116792.5
49	1096717.0
50	1053027.0
51	1009337.0
52	950820.5
53	892304.0
54	820184.5
55	748065.0
56	668820.5
57	589576.0
58	509228.5
59	362421.5
60	295962.0
61	246934.0
62	197906.0
63	161036.5
64	124167.0
65	98744.0
66	73321.0
67	57577.5
68	41834.0
69	31925.5
70	22017.0
71	16598.5
72	11180.0
73	8648.0
74	6116.0
75	4702.5
76	2518.0
77	1747.0
78	1383.5
79	1020.0
80	756.5
81	493.0
82	349.5
83	206.0
84	138.5
85	71.0
86	46.0
87	21.0
88	14.5
89	8.0
90	5.5
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.5230149E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.044690343119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.18086492985411	55.097906413517585
2	11.019648582480087	14.776178538047358
3	3.18262985020547	6.401352983513791
4	1.2804528436859812	3.433902576155711
5	0.6639331638532073	2.225659668953279
6	0.4072818846629932	1.6383652703755371
7	0.2598292012173315	1.2194117836401164
8	0.17486996762657708	0.9379282263868085
9	0.1265491333311214	0.7636002711637602
>10	0.6386125532459078	7.8043709354096755
>50	0.044330277686333575	2.0458887114407407
>100	0.02014336221679661	2.3341055260340084
>500	6.480013273173612E-4	0.2904858062675845
>1k	1.571523541540218E-4	0.18021031077143893
>5k	3.9277001959501216E-5	0.19497060069194122
>10k+	9.819250489875304E-6	0.6556623776308038
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99595	0.6539331952694619	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.252739155736428E-5	0.0	0.0	0.0	0.0
2	7.222516339137588E-5	0.0	0.0	0.0	0.0
3	7.879108733604642E-5	0.0	0.0	6.565923944670535E-6	0.0
4	9.848885917005802E-5	0.0	0.0	6.565923944670535E-6	0.0
5	9.848885917005802E-5	6.565923944670535E-6	0.0	6.565923944670535E-6	0.0
6	9.848885917005802E-5	6.565923944670535E-6	0.0	2.626369577868214E-5	0.0
7	9.848885917005802E-5	6.565923944670535E-6	0.0	2.626369577868214E-5	0.0
8	1.0505478311472856E-4	6.565923944670535E-6	0.0	3.282961972335267E-5	6.565923944670535E-6
9	1.0505478311472856E-4	6.565923944670535E-6	0.0	3.939554366802321E-5	6.565923944670535E-6
10	1.0505478311472856E-4	6.565923944670535E-6	0.0	4.596146761269374E-5	1.313184788934107E-5
11	1.0505478311472856E-4	6.565923944670535E-6	0.0	4.596146761269374E-5	1.313184788934107E-5
12	1.1818663100406963E-4	6.565923944670535E-6	0.0	4.596146761269374E-5	1.313184788934107E-5
13	1.1818663100406963E-4	6.565923944670535E-6	0.0	4.596146761269374E-5	1.9697771834011605E-5
14	1.3788440283808123E-4	6.565923944670535E-6	0.0	5.252739155736428E-5	2.626369577868214E-5
15	1.4445032678275175E-4	6.565923944670535E-6	0.0	5.252739155736428E-5	2.626369577868214E-5
16	1.707140225614339E-4	1.313184788934107E-5	0.0	5.252739155736428E-5	2.626369577868214E-5
17	1.7727994650610444E-4	1.313184788934107E-5	0.0	5.252739155736428E-5	3.282961972335267E-5
18	1.9697771834011605E-4	1.313184788934107E-5	0.0	5.9093315502034815E-5	3.282961972335267E-5
19	2.4293918595280978E-4	1.313184788934107E-5	0.0	5.9093315502034815E-5	3.282961972335267E-5
20	2.7576880567616247E-4	1.313184788934107E-5	0.0	6.565923944670535E-5	3.282961972335267E-5
21	3.217302732888562E-4	1.313184788934107E-5	0.0	7.879108733604642E-5	3.282961972335267E-5
22	3.676917409015499E-4	1.313184788934107E-5	0.0	7.879108733604642E-5	3.282961972335267E-5
23	3.939554366802321E-4	1.313184788934107E-5	0.0	9.848885917005802E-5	3.282961972335267E-5
24	4.79312447960949E-4	1.313184788934107E-5	0.0	1.0505478311472856E-4	3.282961972335267E-5
25	5.318398395183134E-4	1.313184788934107E-5	0.0	1.0505478311472856E-4	4.596146761269374E-5
26	6.040650029096892E-4	1.313184788934107E-5	0.0	1.1162070705939909E-4	4.596146761269374E-5
27	6.959879381350767E-4	1.313184788934107E-5	0.0	1.1818663100406963E-4	4.596146761269374E-5
28	8.273064170284874E-4	1.313184788934107E-5	0.0	1.2475255494874015E-4	4.596146761269374E-5
29	9.32361200143216E-4	1.9697771834011605E-5	0.0	1.313184788934107E-4	4.596146761269374E-5
30	9.980204395899212E-4	1.9697771834011605E-5	0.0	1.3788440283808123E-4	4.596146761269374E-5
31	0.001155602614262014	1.9697771834011605E-5	0.0	1.5758217467209284E-4	4.596146761269374E-5
32	0.0012606573973767427	1.9697771834011605E-5	0.0	1.6414809861676336E-4	4.596146761269374E-5
33	0.0016940083777249979	2.626369577868214E-5	0.0	1.707140225614339E-4	4.596146761269374E-5
34	0.0018384587045077497	2.626369577868214E-5	0.0	1.8384587045077496E-4	4.596146761269374E-5
35	0.0019763431073458308	2.626369577868214E-5	0.0	1.9697771834011605E-4	4.596146761269374E-5
36	0.002147057129907265	2.626369577868214E-5	0.0	2.0354364228478657E-4	4.596146761269374E-5
37	0.0022521119130219933	2.626369577868214E-5	0.0	2.2980733806346872E-4	4.596146761269374E-5
38	0.002442523707417439	2.626369577868214E-5	0.0	2.495051098974803E-4	4.596146761269374E-5
39	0.002606671806034202	2.626369577868214E-5	0.0	2.692028817314919E-4	4.596146761269374E-5
40	0.002764253980706295	2.626369577868214E-5	0.0	2.82334729620833E-4	4.596146761269374E-5
41	0.002974363546935752	2.626369577868214E-5	0.0	3.020325014548446E-4	5.252739155736428E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	58890	0.0	41.497116	1
ATCAACG	62030	0.0	39.214653	11
TCAACGC	60760	0.0	39.183426	12
CAACGCA	60815	0.0	39.16345	13
GGTATCA	62325	0.0	38.931007	8
GTATCAA	62475	0.0	38.845055	9
AGTGGTA	62570	0.0	38.759785	5
AACGCAG	61755	0.0	38.548298	14
GTGGTAT	62915	0.0	38.532307	6
TATCAAC	63780	0.0	38.186577	10
TGGTATC	63590	0.0	38.130684	7
ACGCAGA	62965	0.0	37.826176	15
CGCAGAG	63000	0.0	37.767857	16
AGAGTAC	63780	0.0	37.471775	19
CAGTGGT	65070	0.0	37.26341	4
GCAGTGG	65300	0.0	37.103367	3
GAGTACT	28175	0.0	37.02449	20
CAGAGTA	64700	0.0	36.34691	18
AGCAGTG	67625	0.0	36.022327	2
GTACTTT	28470	0.0	35.823673	22
>>END_MODULE