FastQCFastQC Report
Thu 26 May 2016
SRR805380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17154972
Sequences flagged as poor quality0
Sequence length53
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA14250.027.540351
CTCGTAT14950.026.56521812
GCTCGTA15650.024.77635811
TATGCCG16800.022.80059416
TCGTATG17900.022.18715113
ATGCCGT19650.019.61323217
CGTATGC20850.018.93525314
GCCGTCT21450.018.62470819
TGCCGTC22000.017.94545618
GAGCTCG23550.017.1634839
TCAACGC159250.017.13249611
CAACGCA161000.016.87329312
ATCAACG163350.016.61616110
AGTGGTA167500.016.2886564
AACGCAG170650.015.96044513
AAGCAGT95950.015.8217821
GGTATCA171050.015.8132137
CGCAGAG173200.015.71189415
ACGCAGA173950.015.6441514
GTGGTAT175700.015.5150835
Produced by FastQC (version 0.11.5)