##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805380_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17154972
Sequences flagged as poor quality	0
Sequence length	53
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.164084791278	35.0	28.0	37.0	26.0	37.0
2	35.03113808638102	35.0	33.0	37.0	33.0	37.0
3	37.60844465382981	39.0	37.0	39.0	35.0	39.0
4	38.09193719465121	39.0	39.0	39.0	37.0	39.0
5	38.141390612587415	39.0	39.0	39.0	37.0	39.0
6	38.121475919634264	39.0	39.0	39.0	37.0	39.0
7	38.14624168433502	39.0	39.0	39.0	37.0	39.0
8	39.11451251567184	40.0	40.0	40.0	37.0	40.0
9	39.15447655641758	40.0	40.0	40.0	37.0	40.0
10	39.11475250440514	40.0	40.0	40.0	38.0	40.0
11	39.17967881264977	40.0	40.0	40.0	38.0	40.0
12	39.189791041337756	40.0	40.0	40.0	38.0	40.0
13	39.205745774461185	40.0	40.0	40.0	38.0	40.0
14	39.17209267377411	40.0	40.0	40.0	38.0	40.0
15	39.14869852308707	40.0	40.0	40.0	38.0	40.0
16	39.09735643987061	40.0	40.0	40.0	37.0	40.0
17	39.055696389361636	40.0	40.0	40.0	37.0	40.0
18	39.0397063312024	40.0	40.0	40.0	37.0	40.0
19	39.009700336438904	40.0	40.0	40.0	37.0	40.0
20	38.98284141763682	40.0	40.0	40.0	37.0	40.0
21	38.95810748044357	40.0	40.0	40.0	37.0	40.0
22	38.92430381116332	40.0	40.0	40.0	37.0	40.0
23	38.885510742891334	40.0	40.0	40.0	37.0	40.0
24	38.8505349935867	40.0	40.0	40.0	37.0	40.0
25	38.850317097573814	40.0	40.0	40.0	37.0	40.0
26	38.84743402670666	40.0	40.0	40.0	37.0	40.0
27	38.85454910681288	40.0	40.0	40.0	37.0	40.0
28	38.83697222006541	40.0	40.0	40.0	37.0	40.0
29	38.80974880052267	40.0	40.0	40.0	37.0	40.0
30	38.70758914674999	40.0	39.0	40.0	36.0	40.0
31	38.729898305867245	40.0	39.0	40.0	36.0	40.0
32	38.71607904693753	40.0	39.0	40.0	36.0	40.0
33	38.62336749952142	40.0	39.0	40.0	36.0	40.0
34	38.62500562519134	40.0	39.0	40.0	36.0	40.0
35	38.588742202552126	40.0	39.0	40.0	36.0	40.0
36	38.52768561790716	40.0	39.0	40.0	35.0	40.0
37	38.50675920660203	40.0	39.0	40.0	35.0	40.0
38	38.34795661572633	40.0	39.0	40.0	35.0	40.0
39	38.36678416030058	40.0	38.0	40.0	35.0	40.0
40	38.3790822858819	40.0	38.0	40.0	35.0	40.0
41	38.34690461750681	40.0	38.0	40.0	35.0	40.0
42	38.32175878806447	40.0	38.0	40.0	35.0	40.0
43	38.21084831849332	40.0	38.0	40.0	35.0	40.0
44	38.01968700386104	40.0	38.0	40.0	34.0	40.0
45	37.959886731380266	40.0	38.0	40.0	34.0	40.0
46	37.89991659560855	40.0	38.0	40.0	34.0	40.0
47	38.021161969835916	40.0	38.0	40.0	34.0	40.0
48	38.02398156056448	40.0	38.0	40.0	34.0	40.0
49	37.97103113896076	40.0	38.0	40.0	34.0	40.0
50	37.91093007904647	40.0	38.0	40.0	34.0	40.0
51	37.90318276240847	40.0	38.0	40.0	34.0	40.0
52	37.852560645391904	40.0	38.0	40.0	34.0	40.0
53	37.4499589390178	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	6.0
11	21.0
12	48.0
13	83.0
14	198.0
15	504.0
16	1102.0
17	1888.0
18	3138.0
19	4584.0
20	6379.0
21	8940.0
22	12405.0
23	16679.0
24	23287.0
25	31339.0
26	43132.0
27	56012.0
28	73668.0
29	93908.0
30	118343.0
31	149641.0
32	185654.0
33	231322.0
34	298159.0
35	405513.0
36	595457.0
37	1017423.0
38	2660263.0
39	1.1115874E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.688004853636603	32.5526150669322	19.433112452762966	29.326267626668233
2	22.713648264771287	23.84761688914444	33.522345591703676	19.916389254380597
3	17.54505341075462	27.835953331780434	29.948227254465937	24.670766002999013
4	18.944595187914036	30.512944002473454	29.603604132959237	20.938856676653277
5	23.626619734500295	29.467200529385885	27.52639293144868	19.379786804665144
6	21.747432755938046	28.0321530108006	29.80573795165623	20.414676281605125
7	22.14425648727378	29.13096564657756	28.518029641785485	20.206748224363174
8	20.16366450496101	27.363664598228432	30.669744025230706	21.802926871579857
9	20.214996561929684	26.874389535581873	31.68498322235676	21.225630680131687
10	21.0405764579505	28.272485667711962	29.548768718479984	21.138169155857554
11	21.03384954519308	28.339579918871333	29.27202679199943	21.354543743936162
12	21.111646232940515	28.225088330077135	28.818432347193575	21.84483308978878
13	21.386750150335423	28.670148805838913	28.273616535194577	21.669484508631083
14	21.63146637604538	28.502879515046715	28.22925038875027	21.63640372015763
15	21.54493752598372	28.32127618745166	28.12113013066999	22.012656155894632
16	21.965457011529953	28.280244351316924	28.037591667302053	21.716706969851074
17	22.277401560317323	28.016402475037555	27.884248368344757	21.82194759630036
18	21.79913205337788	28.299982069338263	27.852450006913447	22.048435870370408
19	22.336737127871732	28.208498387522873	27.729471082785796	21.725293401819602
20	22.157593728512058	28.23934658710023	27.894041447575663	21.709018236812046
21	22.125416468181935	28.245956915581093	27.90695898541834	21.721667630818633
22	22.041213474437615	28.129349322167357	28.123042112805546	21.70639509058948
23	21.84830147201639	28.226434878471384	28.300121970470137	21.625141679042088
24	21.78903585502792	28.078850842776077	28.178326376749553	21.953786925446455
25	21.833262100340356	28.095580686462213	28.195347681127082	21.875809532070353
26	21.89194479594604	27.884545658249984	28.612975876614666	21.610533669189316
27	21.986343084675394	27.781071283590553	28.47973753614987	21.752848095584184
28	22.204717093096978	27.848730968491235	28.235202016068577	21.711349922343214
29	22.148978150474395	27.89503824314024	28.342611110061856	21.613372496323517
30	22.04859908835759	27.808882462763563	28.28189401883023	21.860624430048617
31	22.095908987785	27.98852717451244	28.095149324638946	21.820414513063618
32	22.059616302492362	27.944091077502197	28.269466134949102	21.726826485056343
33	22.116608526087948	27.9127765408186	28.052636868191914	21.917978064901533
34	22.054439960613166	28.092951711025815	27.981374729145582	21.871233599215433
35	22.05308758300509	27.96272707411006	28.173435666347924	21.810749676536926
36	22.045888503927607	27.969908665546058	28.143036316235314	21.841166514291015
37	22.05306426614978	28.1021968441569	27.935376402829455	21.909362486863866
38	22.00183713503001	28.04270388782914	28.229186267398166	21.72627270974269
39	22.03702226969534	28.02489563958484	28.128061065911385	21.810021024808435
40	22.09184602574694	28.06785111628279	28.006399544108845	21.833903313861427
41	22.041213474437615	27.926218707905793	28.20095538482954	21.831612432827054
42	22.06943852779241	27.91807529618818	28.168533297518643	21.843952878500765
43	22.007054281405996	28.05725943475746	27.981223169586055	21.95446311425049
44	21.94409294285062	27.93275325660689	28.251751154126048	21.871402646416442
45	22.025795203862764	27.920739246907544	28.143910698309504	21.909554850920188
46	22.183557046901623	27.941969243668836	27.96707566762569	21.90739804180386
47	22.059103331675505	27.919655013135554	28.1750795046474	21.846162150541545
48	22.035914719068035	27.89415803185222	28.084062159938238	21.985865089141505
49	22.07094829417384	28.068148406188016	27.937725576002105	21.92317772363604
50	22.113728894456955	27.99605851877811	28.079725224850264	21.810487361914667
51	22.085888569214802	27.911121044091473	28.080727849628666	21.922262537065055
52	22.102740826391322	28.050252719736296	27.875830983577238	21.971175470295144
53	22.267608481086416	27.91887972769644	28.00097254603505	21.812539245182098
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	519.0
1	557.5
2	596.0
3	693.0
4	790.0
5	892.0
6	994.0
7	1217.0
8	2013.0
9	2586.0
10	3814.5
11	5043.0
12	7696.5
13	10350.0
14	15294.5
15	20239.0
16	30583.0
17	40927.0
18	58882.0
19	76837.0
20	102110.0
21	127383.0
22	171219.5
23	215056.0
24	240535.0
25	305391.5
26	344769.0
27	394371.0
28	443973.0
29	497704.5
30	551436.0
31	602503.5
32	653571.0
33	707837.0
34	762103.0
35	821556.5
36	881010.0
37	934673.5
38	988337.0
39	1045716.0
40	1103095.0
41	1149397.0
42	1215030.5
43	1234362.0
44	1228197.0
45	1222032.0
46	1187030.0
47	1152028.0
48	1120069.0
49	1088110.0
50	1051537.0
51	1014964.0
52	963007.5
53	911051.0
54	845459.0
55	779867.0
56	707144.0
57	634421.0
58	555457.0
59	405835.0
60	335177.0
61	282157.5
62	229138.0
63	191649.5
64	154161.0
65	125512.0
66	96863.0
67	77657.0
68	58451.0
69	46324.5
70	34198.0
71	26429.0
72	18660.0
73	14270.0
74	9880.0
75	7647.0
76	4280.0
77	3146.0
78	2483.0
79	1820.0
80	1412.0
81	1004.0
82	742.0
83	480.0
84	357.5
85	235.0
86	169.0
87	103.0
88	77.0
89	51.0
90	47.0
91	43.0
92	35.0
93	21.0
94	15.0
95	11.0
96	7.0
97	4.0
98	1.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7154972E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.209226258553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.22327874713241	37.185122749850244
2	17.040629440552106	17.793561560998885
3	5.398023173696026	8.454798396732244
4	2.18359463120213	4.560151446295744
5	1.1163321101830468	2.9141417860117302
6	0.679058819800871	2.1271881339509786
7	0.46282981272226476	1.6914790488134304
8	0.3021049338713026	1.2618131877049623
9	0.232062576546444	1.0904226808550461
>10	1.164552606081934	11.313253233946034
>50	0.12155059617489584	4.433114475705696
>100	0.07514428077228613	6.765062959903785
>500	7.93599749883184E-4	0.24821772154255797
>1k	2.2335758501277136E-5	0.03024365076865307
>5k	1.1167877927798265E-5	0.05641749185561652
>10k+	1.1167877927798265E-5	0.0750114750643351
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5738877335387083E-4	0.0	0.0	0.0	0.0
2	1.5738877335387083E-4	0.0	0.0	0.0	0.0
3	1.5738877335387083E-4	1.1658427655842284E-5	0.0	0.0	0.0
4	1.6321798718179197E-4	1.1658427655842284E-5	0.0	0.0	0.0
5	1.690472010097131E-4	1.1658427655842284E-5	0.0	0.0	0.0
6	1.7487641483763424E-4	2.3316855311684567E-5	0.0	5.829213827921142E-6	0.0
7	1.7487641483763424E-4	2.9146069139605708E-5	0.0	5.829213827921142E-6	0.0
8	1.8653484249347654E-4	2.9146069139605708E-5	0.0	1.7487641483763424E-5	0.0
9	1.8653484249347654E-4	2.9146069139605708E-5	0.0	1.7487641483763424E-5	0.0
10	1.981932701493188E-4	2.9146069139605708E-5	0.0	2.3316855311684567E-5	0.0
11	2.1568091163308222E-4	4.080449679544799E-5	0.0	3.497528296752685E-5	0.0
12	2.3316855311684566E-4	4.080449679544799E-5	0.0	4.080449679544799E-5	0.0
13	2.681438360843725E-4	4.080449679544799E-5	0.0	5.8292138279211415E-5	0.0
14	2.7397304991229365E-4	4.6633710623369135E-5	0.0	7.577977976297483E-5	0.0
15	2.9146069139605706E-4	4.6633710623369135E-5	0.0	7.577977976297483E-5	0.0
16	3.089483328798205E-4	4.6633710623369135E-5	0.0	7.577977976297483E-5	0.0
17	3.206067605356628E-4	4.6633710623369135E-5	0.0	8.160899359089598E-5	0.0
18	3.4392361584734734E-4	4.6633710623369135E-5	0.0	8.743820741881712E-5	0.0
19	3.8472811264279535E-4	4.6633710623369135E-5	0.0	9.326742124673827E-5	0.0
20	4.255326094382433E-4	4.6633710623369135E-5	0.0	9.90966350746594E-5	0.0
21	4.663371062336913E-4	4.6633710623369135E-5	0.0	9.90966350746594E-5	0.0
22	5.246292445129027E-4	4.6633710623369135E-5	0.0	9.90966350746594E-5	0.0
23	6.062382381037987E-4	4.6633710623369135E-5	0.0	9.90966350746594E-5	0.0
24	6.761888040388524E-4	4.6633710623369135E-5	0.0	1.0492584890258054E-4	0.0
25	7.752854391135118E-4	4.6633710623369135E-5	0.0	1.107550627305017E-4	0.0
26	8.918697156719347E-4	5.8292138279211415E-5	0.0	1.107550627305017E-4	0.0
27	0.0010142832060582787	5.8292138279211415E-5	0.0	1.2824270421426512E-4	0.0
28	0.001154184337928386	5.8292138279211415E-5	0.0	1.3407191804218625E-4	0.0
29	0.0013290607527660203	5.8292138279211415E-5	0.0	1.399011318701074E-4	0.0
30	0.0014689618846361276	5.8292138279211415E-5	0.0	1.399011318701074E-4	0.0
31	0.0016554967271296041	6.412135210713256E-5	0.0	1.399011318701074E-4	0.0
32	0.0018420315696230807	6.99505659350537E-5	0.0	1.4573034569802853E-4	0.0
33	0.001976103487665267	7.577977976297483E-5	0.0	1.5738877335387083E-4	0.0
34	0.002220930468437955	7.577977976297483E-5	0.0	1.5738877335387083E-4	0.0
35	0.0024249529524151946	7.577977976297483E-5	0.0	1.690472010097131E-4	0.0
36	0.002681438360843725	8.160899359089598E-5	0.0	1.690472010097131E-4	0.0
37	0.0029495821969280976	8.160899359089598E-5	0.0	1.7487641483763424E-4	0.0
38	0.0031885799638728643	8.160899359089598E-5	0.0	1.7487641483763424E-4	0.0
39	0.0035558204350318963	8.743820741881712E-5	0.0	1.9236405632139768E-4	0.0
40	0.003841451912600032	8.743820741881712E-5	0.0	1.9236405632139768E-4	0.0
41	0.004092108107200641	8.743820741881712E-5	0.0	1.981932701493188E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	1425	0.0	27.54035	1
CTCGTAT	1495	0.0	26.565218	12
GCTCGTA	1565	0.0	24.776358	11
TATGCCG	1680	0.0	22.800594	16
TCGTATG	1790	0.0	22.187151	13
ATGCCGT	1965	0.0	19.613232	17
CGTATGC	2085	0.0	18.935253	14
GCCGTCT	2145	0.0	18.624708	19
TGCCGTC	2200	0.0	17.945456	18
GAGCTCG	2355	0.0	17.163483	9
TCAACGC	15925	0.0	17.132496	11
CAACGCA	16100	0.0	16.873293	12
ATCAACG	16335	0.0	16.616161	10
AGTGGTA	16750	0.0	16.288656	4
AACGCAG	17065	0.0	15.960445	13
AAGCAGT	9595	0.0	15.821782	1
GGTATCA	17105	0.0	15.813213	7
CGCAGAG	17320	0.0	15.711894	15
ACGCAGA	17395	0.0	15.64415	14
GTGGTAT	17570	0.0	15.515083	5
>>END_MODULE