FastQCFastQC Report
Thu 26 May 2016
SRR805379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12900008
Sequences flagged as poor quality0
Sequence length53
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT311880.24176729192726082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT157000.031.283441
GATCGGA14000.030.2142871
CTCGTAT13600.030.06617712
GCTCGTA15800.026.62341711
TCGTATG16150.025.75541713
TATGCCG16950.024.81710816
TCAACGC201400.024.29344612
CAACGCA202500.024.1614813
ATCAACG205250.023.89500611
AACGCAG209300.023.37649214
GGTATCA208800.023.3536878
CGCAGAG210800.023.31048416
AGTGGTA211100.023.1771685
GTGGTAT211500.023.0888886
GTATCAA213050.022.976069
ACGCAGA216450.022.72372215
TATCAAC217500.022.46275910
TGGTATC220400.022.1778567
AGAGTAC219550.022.1138719
ATGCCGT19200.021.78645917
Produced by FastQC (version 0.11.5)