##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805379_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12900008
Sequences flagged as poor quality	0
Sequence length	53
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.492562717790562	35.0	28.0	37.0	19.0	37.0
2	34.812233837374364	35.0	33.0	37.0	32.0	37.0
3	37.48927527796882	39.0	37.0	39.0	35.0	39.0
4	38.0508335343668	39.0	39.0	39.0	37.0	39.0
5	38.11190427168727	39.0	39.0	39.0	37.0	39.0
6	38.11666605167997	39.0	39.0	39.0	37.0	39.0
7	38.13575472201258	39.0	39.0	39.0	37.0	39.0
8	39.0495684188723	40.0	40.0	40.0	37.0	40.0
9	39.090130564260114	40.0	40.0	40.0	37.0	40.0
10	39.05861360706133	40.0	40.0	40.0	37.0	40.0
11	39.13131201158945	40.0	40.0	40.0	38.0	40.0
12	39.14653735098459	40.0	40.0	40.0	38.0	40.0
13	39.18104911252768	40.0	40.0	40.0	38.0	40.0
14	39.14631068445849	40.0	40.0	40.0	38.0	40.0
15	39.120014111619156	40.0	40.0	40.0	37.0	40.0
16	39.073329334369404	40.0	40.0	40.0	37.0	40.0
17	39.01917355400089	40.0	40.0	40.0	37.0	40.0
18	38.98437326550495	40.0	40.0	40.0	37.0	40.0
19	38.96554056400585	40.0	40.0	40.0	37.0	40.0
20	38.93456089329557	40.0	40.0	40.0	37.0	40.0
21	38.90205742508066	40.0	40.0	40.0	37.0	40.0
22	38.88372247521087	40.0	40.0	40.0	37.0	40.0
23	38.83253739067449	40.0	40.0	40.0	37.0	40.0
24	38.82907917576485	40.0	40.0	40.0	37.0	40.0
25	38.82594483662336	40.0	40.0	40.0	37.0	40.0
26	38.84674567643679	40.0	40.0	40.0	37.0	40.0
27	38.847035676256944	40.0	40.0	40.0	37.0	40.0
28	38.81722057846786	40.0	40.0	40.0	37.0	40.0
29	38.77813486627295	40.0	40.0	40.0	37.0	40.0
30	38.67389446580188	40.0	39.0	40.0	36.0	40.0
31	38.69822313288488	40.0	39.0	40.0	36.0	40.0
32	38.672673613845824	40.0	39.0	40.0	36.0	40.0
33	38.6274211612892	40.0	39.0	40.0	36.0	40.0
34	38.61489806828027	40.0	39.0	40.0	36.0	40.0
35	38.584671110281484	40.0	39.0	40.0	35.0	40.0
36	38.5105234818459	40.0	39.0	40.0	35.0	40.0
37	38.4738225743736	40.0	39.0	40.0	35.0	40.0
38	38.35315730036756	40.0	39.0	40.0	35.0	40.0
39	38.34241474889008	40.0	39.0	40.0	35.0	40.0
40	38.334880102399936	40.0	38.0	40.0	35.0	40.0
41	38.300384697435845	40.0	38.0	40.0	35.0	40.0
42	38.250606588771106	40.0	38.0	40.0	35.0	40.0
43	38.14954579873129	40.0	38.0	40.0	35.0	40.0
44	38.04574059178878	40.0	38.0	40.0	34.0	40.0
45	37.95692785616877	40.0	38.0	40.0	34.0	40.0
46	37.86181558957173	40.0	38.0	40.0	34.0	40.0
47	37.97278668354314	40.0	38.0	40.0	34.0	40.0
48	37.98113497293955	40.0	38.0	40.0	34.0	40.0
49	37.916855400399754	40.0	38.0	40.0	34.0	40.0
50	37.82165871525041	40.0	38.0	40.0	34.0	40.0
51	37.75783263080147	40.0	38.0	40.0	34.0	40.0
52	37.69247158606413	40.0	37.0	40.0	34.0	40.0
53	37.3190411199745	40.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	12.0
11	32.0
12	55.0
13	132.0
14	292.0
15	602.0
16	1077.0
17	1872.0
18	2707.0
19	3852.0
20	5369.0
21	7550.0
22	10475.0
23	14583.0
24	20229.0
25	27602.0
26	36814.0
27	48358.0
28	62715.0
29	79435.0
30	99090.0
31	122797.0
32	149830.0
33	182335.0
34	229865.0
35	308136.0
36	444961.0
37	763021.0
38	1910164.0
39	8366046.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.766610997450544	35.672225939704845	18.913360363807527	30.647802699037086
2	25.29693780034865	26.20886746736901	29.012516891462393	19.481677840819945
3	19.44435228257223	26.580619174809815	28.245842948314447	25.729185594303505
4	18.784740288533154	31.321089103200556	26.491409927807798	23.402760680458492
5	23.95483010553172	30.111392178981593	25.85435605931407	20.079421656172617
6	23.151621301320123	27.55028524013318	28.136998054574853	21.161095403971842
7	22.809947094606454	28.836648783473624	27.84162614472797	20.511777977191954
8	20.931545158731684	27.28792106175438	29.551617332330338	22.2289164471836
9	21.036087729557998	26.50975875363798	30.73360109544118	21.72055242136284
10	21.17292485400009	28.228191796470202	28.997214575370805	21.6016687741589
11	21.129700074604603	28.776152696959567	28.35985838148317	21.734288846952655
12	21.169475243736283	27.913230751484807	28.544594701026544	22.37269930375237
13	21.465211494442485	28.517121849846916	27.42675043302299	22.590916222687614
14	21.813668642686114	28.968571182281437	27.257246662172612	21.960513512859837
15	21.66964547618885	28.71739304347718	27.185393993554115	22.427567486779854
16	22.27031177034929	27.910556334538704	27.171890125959614	22.64724176915239
17	23.10078412354473	27.649851069859803	27.00560340737773	22.24376139921774
18	21.945071661971063	28.00409116025354	27.03344059941668	23.017396578358714
19	22.521877505812398	28.503687749651007	26.917138345960716	22.057296398575875
20	22.98728806989887	27.97830823050652	27.02451037239667	22.009893327197936
21	22.36298613148147	28.484804040431605	27.109510319683523	22.042699508403405
22	22.74960604675594	27.780075795301833	27.506324027085878	21.963994130856353
23	22.005521236886054	27.937370271398283	28.216284827110183	21.84082366460548
24	21.901823626776046	28.3074243054733	27.41436284380599	22.376389223944667
25	21.881156972925908	27.90083541033463	27.49279690369184	22.72521071304762
26	22.01821115149696	27.90606021329599	28.306331282895332	21.76939735231172
27	22.163660673698807	27.433424847488467	28.37185062210814	22.031063856704584
28	22.619737910240055	27.567440268254096	27.822579644911848	21.990242176594
29	22.716125447364064	27.53147905024555	27.893719135678058	21.85867636671233
30	22.556195313987402	27.44702173828109	27.84592846764126	22.150854480090246
31	22.405544244623723	27.684106862569386	27.77453316308021	22.13581572972668
32	22.36581558709111	27.71916110439621	27.917424547333614	21.997598761179063
33	22.43256748367908	27.637463480642804	27.721688234611946	22.20828080106617
34	22.272776885099606	27.95942452128712	27.623843334050648	22.14395525956263
35	22.29286214396146	27.76420758808832	27.85639357743034	22.086536690519882
36	22.303389269215955	27.70747894109833	27.837176535084318	22.151955254601393
37	22.264714874595427	27.954579563051436	27.632703793672064	22.148001768681073
38	22.314606316523214	27.814044766483864	27.845587382581467	22.02576153441145
39	22.217342810950193	27.742068066934532	27.91909121296669	22.121497909148584
40	22.2401179906245	27.8937036318117	27.75100604588772	22.11517233167607
41	22.292637337899325	27.69304484152258	27.904765640455416	22.10955218012268
42	22.27057533607731	27.674021597505988	27.934308257793326	22.121094808623372
43	22.201559875001628	27.863858689079883	27.7386262086039	22.195955227314588
44	22.195699413519744	27.710571962435992	27.97106017298594	22.122668451058324
45	22.279451299565086	27.638843324748326	27.878300540588814	22.203404835097775
46	22.403722540327106	27.735277373471394	27.661075869100237	22.19992421710126
47	22.34080009872862	27.632339452812744	27.89837804751749	22.128482400941145
48	22.248117985663267	27.662432457406226	27.835897466110097	22.25355209082041
49	22.282505561236864	27.83594397770916	27.64633944413058	22.2352110169234
50	22.359234195823756	27.747540931757563	27.8282463080643	22.06497856435438
51	22.310730349934666	27.643199911193854	27.80164942533369	22.24442031353779
52	22.26267611616985	27.85388970301414	27.600610790318893	22.28282339049712
53	22.491660470288082	27.696145614793416	27.687835542427568	22.12435837249093
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	157.0
1	155.5
2	154.0
3	191.5
4	229.0
5	294.5
6	360.0
7	443.5
8	776.0
9	1025.0
10	1695.0
11	2365.0
12	3693.5
13	5022.0
14	7702.0
15	10382.0
16	16048.0
17	21714.0
18	32324.5
19	42935.0
20	58650.0
21	74365.0
22	118651.5
23	162938.0
24	167198.5
25	197203.5
26	222948.0
27	258211.0
28	293474.0
29	331801.0
30	370128.0
31	407687.5
32	445247.0
33	485932.5
34	526618.0
35	574475.0
36	622332.0
37	665782.5
38	709233.0
39	757434.5
40	805636.0
41	845690.5
42	908197.0
43	930649.0
44	935672.0
45	940695.0
46	922589.0
47	904483.0
48	888183.0
49	871883.0
50	846044.0
51	820205.0
52	778430.0
53	736655.0
54	685820.5
55	634986.0
56	577548.5
57	520111.0
58	456435.0
59	335221.5
60	277684.0
61	234802.5
62	191921.0
63	160292.0
64	128663.0
65	103486.0
66	78309.0
67	62147.5
68	45986.0
69	35777.0
70	25568.0
71	19013.5
72	12459.0
73	9165.0
74	5871.0
75	4396.5
76	2198.5
77	1475.0
78	1130.0
79	785.0
80	604.5
81	424.0
82	329.5
83	235.0
84	175.0
85	115.0
86	88.5
87	62.0
88	50.0
89	38.0
90	30.5
91	23.0
92	21.0
93	15.0
94	11.0
95	11.5
96	12.0
97	9.5
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.2900008E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.8590538134021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26691174910678	38.92227488823522
2	16.580961888322054	17.86069837242214
3	5.179380862113002	8.368696577179476
4	2.051524693605163	4.41972715489615
5	1.0860356113352445	2.924642521708799
6	0.6424489441253806	2.076101535240734
7	0.42214616049332965	1.5915474942591923
8	0.2711125327707302	1.16814915935892
9	0.20127523524267216	0.975644435361622
>10	1.1181951626720792	11.367555731906936
>50	0.11929188041193929	4.4629211833527735
>100	0.06013437250602539	5.321605017702622
>500	4.7996406090101957E-4	0.15655321867947314
>1k	7.210329630782522E-5	0.048387212919772174
>5k	0.0	0.0
>10k+	2.883993858341603E-5	0.3354954967760723
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31188	0.24176729192726082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.976739859386134E-5	7.751933177095704E-6	0.0	0.0	0.0
2	6.976739859386134E-5	7.751933177095704E-6	0.0	1.5503866354191407E-5	0.0
3	6.976739859386134E-5	7.751933177095704E-6	0.0	2.3255799531287114E-5	0.0
4	7.751933177095704E-5	7.751933177095704E-6	0.0	2.3255799531287114E-5	0.0
5	8.527126494805275E-5	7.751933177095704E-6	0.0	2.3255799531287114E-5	0.0
6	8.527126494805275E-5	7.751933177095704E-6	0.0	2.3255799531287114E-5	0.0
7	8.527126494805275E-5	7.751933177095704E-6	0.0	2.3255799531287114E-5	7.751933177095704E-6
8	9.302319812514846E-5	7.751933177095704E-6	0.0	2.3255799531287114E-5	7.751933177095704E-6
9	9.302319812514846E-5	7.751933177095704E-6	0.0	4.651159906257423E-5	7.751933177095704E-6
10	1.0077513130224415E-4	1.5503866354191407E-5	0.0	4.651159906257423E-5	7.751933177095704E-6
11	1.2403093083353126E-4	1.5503866354191407E-5	0.0	4.651159906257423E-5	7.751933177095704E-6
12	1.4728673036481837E-4	1.5503866354191407E-5	0.0	4.651159906257423E-5	7.751933177095704E-6
13	3.255811934380196E-4	3.1007732708382814E-5	0.0	4.651159906257423E-5	7.751933177095704E-6
14	4.2635632474026373E-4	3.875966588547852E-5	0.0	4.651159906257423E-5	7.751933177095704E-6
15	5.581391887508907E-4	3.875966588547852E-5	7.751933177095704E-6	4.651159906257423E-5	7.751933177095704E-6
16	6.046507878134649E-4	3.875966588547852E-5	7.751933177095704E-6	4.651159906257423E-5	7.751933177095704E-6
17	6.124027209905606E-4	3.875966588547852E-5	7.751933177095704E-6	4.651159906257423E-5	7.751933177095704E-6
18	7.674413845324747E-4	4.651159906257423E-5	7.751933177095704E-6	4.651159906257423E-5	7.751933177095704E-6
19	7.984491172408576E-4	4.651159906257423E-5	7.751933177095704E-6	4.651159906257423E-5	7.751933177095704E-6
20	8.294568499492403E-4	5.426353223966993E-5	7.751933177095704E-6	4.651159906257423E-5	7.751933177095704E-6
21	9.379839144285801E-4	6.201546541676563E-5	7.751933177095704E-6	4.651159906257423E-5	7.751933177095704E-6
22	9.844955134911545E-4	6.201546541676563E-5	7.751933177095704E-6	4.651159906257423E-5	1.5503866354191407E-5
23	0.0010387590457308243	6.976739859386134E-5	7.751933177095704E-6	5.426353223966993E-5	1.5503866354191407E-5
24	0.0011627899765643557	7.751933177095704E-5	7.751933177095704E-6	5.426353223966993E-5	1.5503866354191407E-5
25	0.0012480612415124083	7.751933177095704E-5	7.751933177095704E-6	5.426353223966993E-5	1.5503866354191407E-5
26	0.0013255805732833655	8.527126494805275E-5	7.751933177095704E-6	5.426353223966993E-5	1.5503866354191407E-5
27	0.001441859570939801	8.527126494805275E-5	7.751933177095704E-6	5.426353223966993E-5	1.5503866354191407E-5
28	0.001542634702242045	8.527126494805275E-5	7.751933177095704E-6	5.426353223966993E-5	1.5503866354191407E-5
29	0.0018527120293258734	8.527126494805275E-5	7.751933177095704E-6	5.426353223966993E-5	1.5503866354191407E-5
30	0.0019767429601594048	8.527126494805275E-5	7.751933177095704E-6	5.426353223966993E-5	1.5503866354191407E-5
31	0.0021860451559409886	8.527126494805275E-5	7.751933177095704E-6	5.426353223966993E-5	1.5503866354191407E-5
32	0.0024186031512538598	8.527126494805275E-5	7.751933177095704E-6	6.201546541676563E-5	1.5503866354191407E-5
33	0.002651161146566731	8.527126494805275E-5	7.751933177095704E-6	6.976739859386134E-5	1.5503866354191407E-5
34	0.002852711409171219	8.527126494805275E-5	7.751933177095704E-6	6.976739859386134E-5	1.5503866354191407E-5
35	0.0030775174713069944	8.527126494805275E-5	7.751933177095704E-6	6.976739859386134E-5	1.5503866354191407E-5
36	0.003348835132505344	8.527126494805275E-5	7.751933177095704E-6	6.976739859386134E-5	1.5503866354191407E-5
37	0.0036511605264120768	8.527126494805275E-5	7.751933177095704E-6	6.976739859386134E-5	1.5503866354191407E-5
38	0.003875966588547852	9.302319812514846E-5	7.751933177095704E-6	7.751933177095704E-5	1.5503866354191407E-5
39	0.00418604391563168	9.302319812514846E-5	7.751933177095704E-6	7.751933177095704E-5	1.5503866354191407E-5
40	0.004449609643652934	9.302319812514846E-5	7.751933177095704E-6	7.751933177095704E-5	1.5503866354191407E-5
41	0.004775190837090953	9.302319812514846E-5	7.751933177095704E-6	8.527126494805275E-5	1.5503866354191407E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	15700	0.0	31.28344	1
GATCGGA	1400	0.0	30.214287	1
CTCGTAT	1360	0.0	30.066177	12
GCTCGTA	1580	0.0	26.623417	11
TCGTATG	1615	0.0	25.755417	13
TATGCCG	1695	0.0	24.817108	16
TCAACGC	20140	0.0	24.293446	12
CAACGCA	20250	0.0	24.16148	13
ATCAACG	20525	0.0	23.895006	11
AACGCAG	20930	0.0	23.376492	14
GGTATCA	20880	0.0	23.353687	8
CGCAGAG	21080	0.0	23.310484	16
AGTGGTA	21110	0.0	23.177168	5
GTGGTAT	21150	0.0	23.088888	6
GTATCAA	21305	0.0	22.97606	9
ACGCAGA	21645	0.0	22.723722	15
TATCAAC	21750	0.0	22.462759	10
TGGTATC	22040	0.0	22.177856	7
AGAGTAC	21955	0.0	22.11387	19
ATGCCGT	1920	0.0	21.786459	17
>>END_MODULE