FastQCFastQC Report
Thu 26 May 2016
SRR805378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16301323
Sequences flagged as poor quality0
Sequence length53
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGTA34300.036.58600610
CTCGTAT35500.035.41549311
TATGCCG36150.034.84370415
ATGCCGT36700.034.1934616
TCGTATG37550.033.6071912
CGTATGC37650.033.5179313
TGCCGTC40150.031.13823317
GCCGTCT41350.030.12091818
AGCTCGT45050.028.1165399
GAGCTCG45400.028.0033048
ATCGGAA46700.027.1734471
CCGTCTT46000.027.12717619
GTATGCC50250.025.53432814
CGGAAGA51500.024.5495173
TCGGAAG52500.024.1266672
CGTCTTC60600.020.86303520
TCAACGC151700.018.32597211
CAACGCA151800.018.31389812
ATCAACG155950.017.97723610
GCTTGAA70700.017.91584228
Produced by FastQC (version 0.11.5)