##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805378_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16301323
Sequences flagged as poor quality	0
Sequence length	53
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.85275256492985	37.0	35.0	37.0	28.0	37.0
2	35.92580589931259	37.0	35.0	37.0	33.0	37.0
3	38.07815500619183	39.0	38.0	39.0	37.0	39.0
4	38.25766534409508	39.0	39.0	39.0	37.0	39.0
5	38.256946997492165	39.0	39.0	39.0	37.0	39.0
6	38.220736255578764	39.0	39.0	39.0	37.0	39.0
7	38.22503486373468	39.0	39.0	39.0	37.0	39.0
8	39.1772612566477	40.0	40.0	40.0	38.0	40.0
9	39.18487806173769	40.0	40.0	40.0	38.0	40.0
10	39.16481177632024	40.0	40.0	40.0	38.0	40.0
11	39.22108800616981	40.0	40.0	40.0	38.0	40.0
12	39.22597159752003	40.0	40.0	40.0	38.0	40.0
13	39.25507770136203	40.0	40.0	40.0	38.0	40.0
14	39.20307511236972	40.0	40.0	40.0	38.0	40.0
15	39.179666582890235	40.0	40.0	40.0	38.0	40.0
16	39.15769437854829	40.0	40.0	40.0	38.0	40.0
17	39.10511919799393	40.0	40.0	40.0	37.0	40.0
18	39.06387187101317	40.0	40.0	40.0	37.0	40.0
19	39.03930036844249	40.0	40.0	40.0	37.0	40.0
20	38.992871314800645	40.0	40.0	40.0	37.0	40.0
21	38.98203378952739	40.0	40.0	40.0	37.0	40.0
22	38.93692272706945	40.0	40.0	40.0	37.0	40.0
23	38.95947439358143	40.0	40.0	40.0	37.0	40.0
24	38.97880405167114	40.0	40.0	40.0	37.0	40.0
25	38.95862967686733	40.0	40.0	40.0	37.0	40.0
26	38.95662726270745	40.0	40.0	40.0	37.0	40.0
27	38.94696258702438	40.0	40.0	40.0	37.0	40.0
28	38.92162617721273	40.0	40.0	40.0	37.0	40.0
29	38.87042290984603	40.0	40.0	40.0	37.0	40.0
30	38.818686924981485	40.0	40.0	40.0	37.0	40.0
31	38.81285291997465	40.0	40.0	40.0	37.0	40.0
32	38.806895980160625	40.0	40.0	40.0	37.0	40.0
33	38.396630506615935	40.0	39.0	40.0	35.0	40.0
34	38.592100592080776	40.0	39.0	40.0	36.0	40.0
35	38.67111086627754	40.0	39.0	40.0	36.0	40.0
36	38.65736063263086	40.0	39.0	40.0	36.0	40.0
37	38.6323826599841	40.0	39.0	40.0	36.0	40.0
38	38.526077116562874	40.0	39.0	40.0	35.0	40.0
39	38.50822169464405	40.0	39.0	40.0	35.0	40.0
40	38.48967884385826	40.0	39.0	40.0	35.0	40.0
41	38.47186740609949	40.0	39.0	40.0	35.0	40.0
42	38.393361324108476	40.0	39.0	40.0	35.0	40.0
43	38.34328587931176	40.0	39.0	40.0	35.0	40.0
44	38.2397057588516	40.0	38.0	40.0	35.0	40.0
45	37.51871096597497	40.0	38.0	40.0	33.0	40.0
46	37.89037951091454	40.0	38.0	40.0	34.0	40.0
47	38.0029801262143	40.0	38.0	40.0	34.0	40.0
48	37.95638096368006	40.0	38.0	40.0	34.0	40.0
49	38.0007984014549	40.0	38.0	40.0	34.0	40.0
50	37.9951723550291	40.0	38.0	40.0	34.0	40.0
51	38.05011899954378	40.0	38.0	40.0	35.0	40.0
52	38.005754440912554	40.0	38.0	40.0	34.0	40.0
53	37.62581754867381	40.0	38.0	40.0	34.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	12.0
13	45.0
14	129.0
15	336.0
16	719.0
17	1403.0
18	2310.0
19	3276.0
20	4778.0
21	6931.0
22	9614.0
23	13596.0
24	19541.0
25	27140.0
26	37167.0
27	49820.0
28	66022.0
29	85344.0
30	109707.0
31	138376.0
32	167644.0
33	204496.0
34	260953.0
35	350962.0
36	507946.0
37	856810.0
38	2226492.0
39	1.1149751E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.005274295834761	30.242226351812057	24.188024493472092	31.564474858881088
2	23.100763048496127	24.98708847128543	32.64744217386528	19.264706306353172
3	14.50049176990113	26.255733967114203	34.40690059328313	24.836873669701536
4	16.527118688464736	29.48861267272601	33.316240651142245	20.668027987667013
5	22.52620232112449	28.72044189296783	30.27966503086897	18.473690755038717
6	20.62249180633989	28.021492488677147	31.40866541936504	19.947350285617922
7	22.20589703056617	29.075578712230904	29.360972725956046	19.35755153124688
8	20.079081924822912	26.84896189100725	31.002557277099534	22.069398907070305
9	19.967716730721794	26.180641902500795	32.43790703368064	21.41373433309677
10	21.36122325776871	27.67203005547464	29.435917563255448	21.530829123501203
11	21.096030058419185	27.97741017707581	29.170957473819765	21.755602290685243
12	21.31742313185255	27.809006667741016	29.014841310732876	21.858728889673557
13	21.555667598267945	28.222114241893127	28.281109453508773	21.941108706330155
14	21.896940512128985	28.239885805587683	28.16597155948631	21.697202122797027
15	21.394668395933262	28.086904357394793	28.282851643391155	22.23557560328079
16	21.906087009011475	28.15151261035684	28.066899846104516	21.875500534527166
17	22.32750679193339	27.628966066128495	27.972269490028506	22.071257651909605
18	21.89305739172213	27.96501854481382	27.790308798862522	22.35161526460153
19	22.257113732425278	27.89368077670751	27.76618192278013	22.083023568087082
20	22.32672157959204	27.8555489023805	27.78464668174479	22.033082836282674
21	22.324243253139638	27.9183168139175	27.890926399041355	21.866513533901514
22	22.147736107063213	27.729037698351235	28.279557432240317	21.84366876234524
23	21.97899520180049	27.906606107982768	28.17594621001007	21.938452480206667
24	21.856821069062924	27.882724610757055	28.152027905955855	22.10842641422417
25	21.887475022732815	27.818398543480182	28.202134268488514	22.091992165298485
26	21.93481473865649	27.530618220373892	28.595930526620446	21.93863651434917
27	22.125639741019793	27.402009027120066	28.585949741625267	21.88640149023487
28	22.473936624653103	27.414799400024158	28.03630723714879	22.074956738173952
29	22.267168131077458	27.624021682166532	28.060642685259353	22.048167501496657
30	22.19468935128762	27.456489267772927	28.306236248432104	22.04258513250734
31	22.18444478402152	27.579902563736695	28.050987027249263	22.184665624992522
32	22.38469846895249	27.715002027749524	27.92169077319675	21.978608730101232
33	22.367503545571118	27.714167739636835	27.922426909766774	21.995901805025273
34	22.15310376955294	28.016738273329107	27.717406740544924	22.112751216573034
35	22.244673024391947	27.788824256779648	27.918053031646572	22.048449687181833
36	22.08729929466461	27.82463116643968	27.924334730377403	22.163734808518303
37	22.158256725543072	27.88303746879931	27.74123302752789	22.217472778129725
38	22.21471840046357	27.843531472874933	27.85976941871528	22.08198070794622
39	22.19867675770856	27.832133624982465	27.90335483813185	22.065834779177127
40	22.291411562116767	27.763679058442065	27.86876255381235	22.076146825628815
41	22.268996203559674	27.691641960594243	27.89501809147638	22.144343744369703
42	22.221619680807503	27.633904315619045	28.04876021412495	22.0957157894485
43	22.22839213725168	27.697960466153575	27.8346978340347	22.238949562560045
44	22.188407652556787	27.711327479370844	27.998224438593112	22.102040429479253
45	22.230827522404162	27.704131744398907	27.805283043591	22.25975768960593
46	22.201952565445147	27.63218666362233	27.842819874190578	22.32304089674194
47	22.32021290541878	27.616335189481244	27.92864112931202	22.13481077578795
48	22.178052664805183	27.616451744438166	27.93264693914721	22.272848651609443
49	22.252604895933906	27.661190444481104	27.719198006198635	22.367006653386355
50	22.255819358956323	27.709057724946618	27.85887378588842	22.17624913020863
51	22.24907757486923	27.597520765645832	27.88504957542403	22.268352084060908
52	22.23205441668753	27.717971111915276	27.69931618433669	22.350658287060504
53	22.400341371065405	27.64662720933755	27.737178141921365	22.215853277675684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	533.0
1	640.5
2	748.0
3	731.5
4	715.0
5	832.0
6	949.0
7	1145.0
8	1675.0
9	2009.0
10	3063.0
11	4117.0
12	6249.5
13	8382.0
14	12120.0
15	15858.0
16	23949.0
17	32040.0
18	45914.0
19	59788.0
20	79599.0
21	99410.0
22	133962.5
23	168515.0
24	190910.5
25	249528.5
26	285751.0
27	335832.0
28	385913.0
29	441696.5
30	497480.0
31	559538.0
32	621596.0
33	681193.5
34	740791.0
35	807081.0
36	873371.0
37	919572.0
38	965773.0
39	1011199.0
40	1056625.0
41	1101028.0
42	1169525.0
43	1193619.0
44	1203902.5
45	1214186.0
46	1188100.5
47	1162015.0
48	1135842.5
49	1109670.0
50	1067304.0
51	1024938.0
52	953839.0
53	882740.0
54	809559.0
55	736378.0
56	657461.5
57	578545.0
58	501032.0
59	359672.0
60	295825.0
61	248190.5
62	200556.0
63	166171.0
64	131786.0
65	105422.0
66	79058.0
67	62212.0
68	45366.0
69	34009.5
70	22653.0
71	16736.0
72	10819.0
73	7755.0
74	4691.0
75	3384.0
76	1563.5
77	1050.0
78	838.0
79	626.0
80	476.0
81	326.0
82	240.0
83	154.0
84	136.5
85	119.0
86	91.0
87	63.0
88	50.5
89	38.0
90	31.5
91	25.0
92	24.0
93	16.0
94	9.0
95	7.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.6301323E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.4346223509049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.73901738192976	40.13955563712159
2	15.078965898511246	16.416356282552663
3	4.95623304372566	8.093720220548468
4	2.114431945130322	4.603932176794334
5	1.1779122981709906	3.205960555671219
6	0.6960247840050061	2.2732707758509596
7	0.44251301159748335	1.6861620070169427
8	0.32585838519068583	1.419038251018455
9	0.22031838499854065	1.079365327592115
>10	1.1039271745288006	10.913629856585011
>50	0.09680148368775882	3.632713614457411
>100	0.043096586460781496	4.503793356957704
>500	0.004356854975391435	1.59056146472582
>1k	5.201640744345608E-4	0.34412474946829785
>5k	2.260301271010718E-5	0.09781572363895734
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.907577133463339E-5	0.0	0.0	0.0	0.0
2	4.907577133463339E-5	0.0	0.0	0.0	0.0
3	4.907577133463339E-5	0.0	0.0	0.0	0.0
4	4.907577133463339E-5	0.0	0.0	0.0	0.0
5	1.9016861392170438E-4	0.0	0.0	6.134471416829174E-6	1.2268942833658348E-5
6	1.9016861392170438E-4	0.0	0.0	6.134471416829174E-6	1.2268942833658348E-5
7	1.9630308533853357E-4	0.0	0.0	6.134471416829174E-6	1.8403414250487523E-5
8	1.9630308533853357E-4	0.0	0.0	1.2268942833658348E-5	1.8403414250487523E-5
9	1.9630308533853357E-4	6.134471416829174E-6	0.0	1.2268942833658348E-5	1.8403414250487523E-5
10	2.2084097100585025E-4	6.134471416829174E-6	0.0	1.2268942833658348E-5	1.8403414250487523E-5
11	2.392443852563378E-4	6.134471416829174E-6	0.0	1.2268942833658348E-5	1.8403414250487523E-5
12	3.067235708414587E-4	1.2268942833658348E-5	0.0	1.2268942833658348E-5	2.4537885667316696E-5
13	6.011781988492591E-4	1.2268942833658348E-5	0.0	1.2268942833658348E-5	3.067235708414587E-5
14	7.852123413541343E-4	1.2268942833658348E-5	0.0	1.2268942833658348E-5	3.067235708414587E-5
15	8.894983554402303E-4	1.2268942833658348E-5	0.0	1.2268942833658348E-5	3.067235708414587E-5
16	0.0010060533123599845	1.8403414250487523E-5	0.0	1.2268942833658348E-5	3.067235708414587E-5
17	0.001024456726610472	2.4537885667316696E-5	0.0	1.2268942833658348E-5	3.067235708414587E-5
18	0.001122608269279739	2.4537885667316696E-5	0.0	1.2268942833658348E-5	3.067235708414587E-5
19	0.0011532806263638846	2.4537885667316696E-5	0.0	1.8403414250487523E-5	3.067235708414587E-5
20	0.0011655495691975431	2.4537885667316696E-5	0.0	1.8403414250487523E-5	3.6806828500975046E-5
21	0.0012207598119490055	2.4537885667316696E-5	6.134471416829174E-6	1.8403414250487523E-5	3.6806828500975046E-5
22	0.0012452976976163224	2.4537885667316696E-5	6.134471416829174E-6	1.8403414250487523E-5	3.6806828500975046E-5
23	0.0012882389975341266	2.4537885667316696E-5	6.134471416829174E-6	1.8403414250487523E-5	3.6806828500975046E-5
24	0.0013189113546182723	2.4537885667316696E-5	6.134471416829174E-6	1.8403414250487523E-5	3.6806828500975046E-5
25	0.0013925250116202225	2.4537885667316696E-5	6.134471416829174E-6	1.8403414250487523E-5	3.6806828500975046E-5
26	0.0015274833827904643	2.4537885667316696E-5	6.134471416829174E-6	1.8403414250487523E-5	3.6806828500975046E-5
27	0.0016747106967943644	2.4537885667316696E-5	6.134471416829174E-6	1.8403414250487523E-5	3.6806828500975046E-5
28	0.001699248582461681	2.4537885667316696E-5	6.134471416829174E-6	1.8403414250487523E-5	3.6806828500975046E-5
29	0.0019323584963011897	3.6806828500975046E-5	6.134471416829174E-6	1.8403414250487523E-5	3.6806828500975046E-5
30	0.0019937032104694816	3.6806828500975046E-5	6.134471416829174E-6	2.4537885667316696E-5	3.6806828500975046E-5
31	0.002110258167389236	3.6806828500975046E-5	6.134471416829174E-6	3.067235708414587E-5	3.6806828500975046E-5
32	0.0022022752386416733	3.6806828500975046E-5	6.134471416829174E-6	3.067235708414587E-5	3.6806828500975046E-5
33	0.002245216538559478	3.6806828500975046E-5	6.134471416829174E-6	3.067235708414587E-5	3.6806828500975046E-5
34	0.002337233609811915	3.6806828500975046E-5	6.134471416829174E-6	3.067235708414587E-5	3.6806828500975046E-5
35	0.0024476540953148403	3.6806828500975046E-5	6.134471416829174E-6	3.067235708414587E-5	3.6806828500975046E-5
36	0.0025887469379019114	3.6806828500975046E-5	6.134471416829174E-6	3.067235708414587E-5	4.2941299917804216E-5
37	0.0026071503521523987	3.6806828500975046E-5	6.134471416829174E-6	3.067235708414587E-5	4.2941299917804216E-5
38	0.002650091652070203	3.6806828500975046E-5	6.134471416829174E-6	3.067235708414587E-5	4.2941299917804216E-5
39	0.002711436366238495	3.6806828500975046E-5	6.134471416829174E-6	3.6806828500975046E-5	4.2941299917804216E-5
40	0.002785050023240445	4.2941299917804216E-5	6.134471416829174E-6	3.6806828500975046E-5	4.2941299917804216E-5
41	0.002846394737408737	4.2941299917804216E-5	6.134471416829174E-6	3.6806828500975046E-5	4.2941299917804216E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGTA	3430	0.0	36.586006	10
CTCGTAT	3550	0.0	35.415493	11
TATGCCG	3615	0.0	34.843704	15
ATGCCGT	3670	0.0	34.19346	16
TCGTATG	3755	0.0	33.60719	12
CGTATGC	3765	0.0	33.51793	13
TGCCGTC	4015	0.0	31.138233	17
GCCGTCT	4135	0.0	30.120918	18
AGCTCGT	4505	0.0	28.116539	9
GAGCTCG	4540	0.0	28.003304	8
ATCGGAA	4670	0.0	27.173447	1
CCGTCTT	4600	0.0	27.127176	19
GTATGCC	5025	0.0	25.534328	14
CGGAAGA	5150	0.0	24.549517	3
TCGGAAG	5250	0.0	24.126667	2
CGTCTTC	6060	0.0	20.863035	20
TCAACGC	15170	0.0	18.325972	11
CAACGCA	15180	0.0	18.313898	12
ATCAACG	15595	0.0	17.977236	10
GCTTGAA	7070	0.0	17.915842	28
>>END_MODULE