FastQCFastQC Report
Thu 26 May 2016
SRR805377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17654642
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1612100.9131309487895591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT754600.042.0514831
TCAACGC776700.040.75511612
CAACGCA778750.040.64481413
ATCAACG780750.040.6039111
GTGGTAT782400.040.317046
AGTGGTA783100.040.3050085
GGTATCA786000.040.2101148
GTATCAA788150.040.1242759
AACGCAG790800.040.01953514
CGCAGAG794800.039.7737816
TATCAAC796600.039.71340610
TGGTATC796000.039.6695337
ACGCAGA802200.039.4508215
AGAGTAC790950.039.43852219
GCAGTGG805000.039.3865853
AGCAGTG814500.039.0166362
CAGTGGT817550.038.6727374
CAGAGTA809800.038.62206618
GAGTACT291700.038.56513620
GCAGAGT839000.037.5047717
Produced by FastQC (version 0.11.5)