FastQCFastQC Report
Thu 26 May 2016
SRR805376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18715681
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2933031.5671510964522213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG396400.21180100259242501No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC246790.1318626877643405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG244900.13085283939173786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1523350.044.55952512
GTATCAA1520800.044.399379
AAGCAGT1534050.044.3850631
GGTATCA1522250.044.352448
GTGGTAT1528900.044.2071766
ACGCAGA1535450.044.16399415
TGGTATC1528850.044.1609767
AGTGGTA1527150.044.1424265
ATCAACG1537600.044.08542611
CAACGCA1540300.044.07375313
CGCAGAG1550200.043.75438716
AACGCAG1552350.043.7195214
TATCAAC1545700.043.66436410
AGAGTAC1519900.043.51806319
CAGTGGT1567450.043.164924
AGCAGTG1581000.043.1279262
GCAGTGG1585700.042.9556353
CAGAGTA1548500.042.92373718
GCAGAGT1560150.042.85777317
GTACATG1032150.042.3462222
Produced by FastQC (version 0.11.5)