##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805376_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18715681
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.53206468949754	37.0	35.0	37.0	33.0	37.0
2	35.616710928124924	37.0	35.0	37.0	33.0	37.0
3	37.299875382573575	39.0	37.0	39.0	34.0	39.0
4	37.26204384441047	39.0	37.0	39.0	34.0	39.0
5	37.22150815671628	39.0	37.0	39.0	34.0	39.0
6	37.189738754363255	39.0	37.0	39.0	34.0	39.0
7	36.963756648769554	39.0	37.0	39.0	33.0	39.0
8	37.95183060664477	40.0	38.0	40.0	33.0	40.0
9	37.879236133593004	40.0	38.0	40.0	33.0	40.0
10	37.608248986504954	40.0	37.0	40.0	33.0	40.0
11	37.685521996234066	40.0	37.0	40.0	33.0	40.0
12	37.63663208407966	40.0	37.0	40.0	33.0	40.0
13	37.72999785580872	40.0	37.0	40.0	33.0	40.0
14	37.67138577538269	40.0	37.0	40.0	33.0	40.0
15	37.59311707653064	40.0	37.0	40.0	32.0	40.0
16	37.57890845649699	40.0	37.0	40.0	32.0	40.0
17	37.523579932784706	40.0	37.0	40.0	32.0	40.0
18	37.48432819516426	40.0	37.0	40.0	32.0	40.0
19	37.43507676797868	40.0	37.0	40.0	32.0	40.0
20	37.18891778503812	39.0	37.0	40.0	32.0	40.0
21	37.15045869824347	39.0	37.0	40.0	32.0	40.0
22	37.04391835915562	39.0	36.0	40.0	31.0	40.0
23	36.70129513320942	39.0	36.0	40.0	30.0	40.0
24	36.75130068737547	39.0	36.0	40.0	30.0	40.0
25	36.70619236350523	39.0	36.0	40.0	30.0	40.0
26	36.67752121870425	39.0	36.0	40.0	30.0	40.0
27	36.653545655111344	39.0	36.0	40.0	30.0	40.0
28	36.561598533336834	39.0	36.0	40.0	30.0	40.0
29	36.52637432749575	39.0	36.0	40.0	30.0	40.0
30	36.46002477815261	39.0	36.0	40.0	30.0	40.0
31	36.32565205615548	39.0	35.0	40.0	30.0	40.0
32	36.196107478002006	39.0	35.0	40.0	30.0	40.0
33	36.066792012537505	38.0	35.0	40.0	29.0	40.0
34	36.044716833974675	38.0	35.0	40.0	29.0	40.0
35	35.97843017307252	38.0	35.0	40.0	29.0	40.0
36	35.89658666441259	38.0	35.0	40.0	29.0	40.0
37	35.79885653105543	38.0	35.0	40.0	28.0	40.0
38	35.70141759736127	38.0	35.0	40.0	28.0	40.0
39	35.89354376151207	38.0	35.0	40.0	29.0	40.0
40	35.99408715076945	38.0	35.0	40.0	29.0	40.0
41	35.97740926445583	38.0	35.0	40.0	29.0	40.0
42	35.90976518567505	38.0	35.0	40.0	29.0	40.0
43	35.88838808483646	38.0	35.0	40.0	28.0	40.0
44	35.745957734586305	38.0	35.0	40.0	28.0	40.0
45	35.594944474636	38.0	34.0	40.0	28.0	40.0
46	35.524907963541374	38.0	34.0	40.0	28.0	40.0
47	35.433420670078746	38.0	34.0	40.0	27.0	40.0
48	35.31476081474139	38.0	34.0	40.0	27.0	40.0
49	35.14859673019646	38.0	34.0	40.0	27.0	40.0
50	35.00941622161651	38.0	34.0	40.0	26.0	40.0
51	34.83681373923824	37.0	33.0	40.0	26.0	40.0
52	34.481115755285636	37.0	33.0	40.0	25.0	40.0
53	33.99446213044559	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	7.0
12	30.0
13	113.0
14	286.0
15	815.0
16	1939.0
17	4181.0
18	8126.0
19	14977.0
20	25524.0
21	39381.0
22	57080.0
23	77244.0
24	101645.0
25	130744.0
26	164255.0
27	205065.0
28	252126.0
29	311308.0
30	379160.0
31	465023.0
32	572619.0
33	716047.0
34	919842.0
35	1222824.0
36	1710260.0
37	2542649.0
38	3848750.0
39	4943660.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.469572333488692	32.21511950326574	30.406630675100736	22.908677488144836
2	26.02541152523384	34.87815912228895	20.136323118565656	18.960106233911553
3	30.970419938232546	24.275905322387146	22.371005361760545	22.382669377619763
4	21.581175699671306	24.14588600863629	22.99077976377135	31.28215852792105
5	22.609580704009648	32.0197058284975	23.941116542860502	21.429596924632342
6	30.340151662127603	24.096285889890943	23.009929481059224	22.55363296692223
7	22.891120018555565	23.78228182025543	31.73153036750306	21.595067793685946
8	29.529692240426623	24.45859704490582	23.999062604240798	22.01264811042676
9	29.45821207360822	23.988253486474793	24.49178312026156	22.061751319655425
10	21.994625789999304	24.38861294975053	31.64809765671898	21.968663603531176
11	21.752791148769848	32.05478870899755	24.14048946442291	22.051930677809693
12	21.63859279285643	24.298538749404845	31.93742723013926	22.125441227599467
13	21.767725149835584	24.3328842802995	24.20996596383535	29.68942460602956
14	22.04038955355138	31.990366794561204	24.005372820791294	21.963870831096127
15	21.81881599713096	31.8785835257611	24.068779543741954	22.233820933365983
16	21.990565024056565	24.168653013481048	24.054695097656346	29.786086864806045
17	29.868547129009094	23.98127003767589	23.998416087557807	22.151766745757207
18	22.131281250198697	24.00812452402881	23.983401939795833	29.877192285976662
19	22.143153647468132	31.687385567215	23.96634672283632	22.203114062480548
20	29.99044491087447	24.04661096756244	23.93913959101996	22.02380453054313
21	22.344150875407635	31.61036993524307	23.94372398204479	22.101755207304503
22	29.797505097463457	23.902956029224907	24.19968047115144	22.0998584021602
23	22.35211211390064	23.94276222168993	31.63905176627022	22.066073898139212
24	22.206512282401054	31.491800912828126	24.04051447553525	22.261172329235578
25	22.076647918929588	24.06722469783493	24.024730919489386	29.831396463746096
26	22.308271871058285	28.970722465295278	26.54105933949184	22.179946324154596
27	22.38007262466164	23.931734036287537	31.569179876489667	22.119013462561156
28	27.42138530786029	23.855717566462047	26.546466569931386	22.176430555746272
29	27.560723010827125	23.938519789902383	26.33866755903779	22.1620896402327
30	27.421112809093078	23.945081132767758	26.38711890847039	22.246687149668773
31	25.77749107820335	24.56158554957204	26.883643721005928	22.777279651218677
32	24.73908376617447	25.306629237803314	26.937641221818215	23.016645774203994
33	24.349506705099323	24.99119321386168	27.29943409486409	23.359865986174906
34	23.733814441483588	24.926301105474067	27.606299765421305	23.733584687621036
35	23.69932998964879	25.093161183929137	27.70864175340454	23.49886707301754
36	23.79164829748915	25.08674410511699	27.755661148531008	23.365946448862854
37	24.112699933280545	24.859047341103967	27.668824874713348	23.35942785090214
38	24.22946298347359	25.018389659451877	27.52001383225115	23.232133524823382
39	24.255179386739922	24.94718733451377	27.536027142159565	23.26160613658675
40	23.76439307765504	25.311705195231742	27.39399116708604	23.52991056002718
41	24.102435813048963	25.470716240568535	27.25042171855782	23.176426227824678
42	24.019521384233894	25.431631368369658	27.351588221662894	23.197259025733555
43	23.830658366104874	25.226851216367706	27.785016211806557	23.15747420572086
44	24.20694710494371	24.97972689318652	27.38465140541774	23.428674596452034
45	24.187204302103673	24.78398194540717	27.443254669707184	23.585559082781973
46	23.805492303486044	24.923218129225432	27.31700759379261	23.954281973495913
47	23.877741878588335	25.003968597242064	27.356840501823044	23.761449022346557
48	23.81998282616593	24.857722249059492	27.411238736116523	23.911056188658055
49	23.521895890403346	25.50051478222994	27.20524035433175	23.772348973034966
50	24.165425773179187	25.27816113129947	27.09411963155388	23.462293463967463
51	23.983888163086345	25.048551532802893	27.339031905918894	23.628528398191868
52	24.239224851075416	24.973352559279036	27.165936414496482	23.621486175149066
53	24.590737574550452	25.139699698878175	26.61177544113944	23.657787285431933
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4930.0
1	3070.0
2	1210.0
3	1126.0
4	1042.0
5	1145.5
6	1249.0
7	1514.0
8	2251.0
9	2723.0
10	3610.0
11	4497.0
12	6145.0
13	7793.0
14	9954.5
15	12116.0
16	17198.5
17	22281.0
18	30265.5
19	38250.0
20	53395.5
21	68541.0
22	247604.0
23	426667.0
24	316499.5
25	215132.0
26	223932.0
27	247173.0
28	270414.0
29	292527.0
30	314640.0
31	337456.0
32	360272.0
33	401108.0
34	441944.0
35	498071.0
36	554198.0
37	612673.5
38	671149.0
39	730751.0
40	790353.0
41	848385.5
42	964501.0
43	1022584.0
44	1090926.0
45	1159268.0
46	1208910.5
47	1258553.0
48	1315940.0
49	1373327.0
50	1387815.5
51	1402304.0
52	1394414.5
53	1386525.0
54	1348336.0
55	1310147.0
56	1258577.5
57	1207008.0
58	1094332.0
59	872994.0
60	764332.0
61	660606.0
62	556880.0
63	473242.0
64	389604.0
65	319446.5
66	249289.0
67	200015.5
68	150742.0
69	118070.5
70	85399.0
71	64870.0
72	44341.0
73	32582.5
74	20824.0
75	15680.5
76	7742.5
77	4948.0
78	3656.0
79	2364.0
80	1845.0
81	1326.0
82	999.5
83	673.0
84	425.0
85	177.0
86	120.5
87	64.0
88	50.0
89	36.0
90	32.5
91	29.0
92	18.5
93	5.0
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8715681E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.67789587417371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.05741348313366	39.557854727055705
2	11.466275621008732	11.62173443972114
3	3.8447811530243623	5.8453625679586265
4	1.8710143526398832	3.792762821686742
5	1.0549287013996436	2.6730783442104213
6	0.6910314687845047	2.101201249250307
7	0.46298959387249583	1.6424336900367424
8	0.36656288446586516	1.4861308552238306
9	0.2733466120695931	1.2467368029618895
>10	1.7094368409968501	16.063127722240093
>50	0.13682012101524124	4.7474683394741675
>100	0.06296782071645331	5.73141151252367
>500	0.0018510588446744866	0.5868507601351339
>1k	4.642773520617694E-4	0.5268361323727112
>5k	5.273212543256112E-5	0.18593698829834393
>10k+	6.327855051907286E-5	2.191073046850505
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	293303	1.5671510964522213	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	39640	0.21180100259242501	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	24679	0.1318626877643405	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	24490	0.13085283939173786	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4578320179746596E-4	5.343113082553608E-6	0.0	0.0	2.1372452330214434E-5
2	5.075957428425928E-4	5.343113082553608E-6	0.0	0.0	2.6715565412768042E-5
3	5.075957428425928E-4	5.343113082553608E-6	0.0	0.0	2.6715565412768042E-5
4	5.075957428425928E-4	5.343113082553608E-6	0.0	5.343113082553608E-6	5.3431130825536084E-5
5	8.388687539609165E-4	5.343113082553608E-6	0.0	5.343113082553608E-6	9.083292240341134E-5
6	8.816136586213453E-4	5.343113082553608E-6	0.0	1.0686226165107217E-5	9.083292240341134E-5
7	8.86956771703899E-4	5.343113082553608E-6	0.0	1.0686226165107217E-5	9.617603548596495E-5
8	8.86956771703899E-4	5.343113082553608E-6	0.0	1.0686226165107217E-5	1.1754848781617938E-4
9	9.029861109515598E-4	5.343113082553608E-6	0.0	1.0686226165107217E-5	1.5495027939405463E-4
10	9.136723371166671E-4	5.343113082553608E-6	0.0	1.0686226165107217E-5	1.7097961864171547E-4
11	9.350447894468815E-4	5.343113082553608E-6	0.0	1.0686226165107217E-5	1.7632273172426906E-4
12	9.93819033354971E-4	5.343113082553608E-6	0.0	1.0686226165107217E-5	1.976951840544835E-4
13	0.0011113675211711505	5.343113082553608E-6	0.0	1.0686226165107217E-5	2.1906763638469793E-4
14	0.0011380830865839187	5.343113082553608E-6	0.0	2.1372452330214434E-5	2.2975386254980515E-4
15	0.0012235728959047764	5.343113082553608E-6	0.0	2.1372452330214434E-5	2.3509697563235877E-4
16	0.001255631574400098	5.343113082553608E-6	0.0	2.1372452330214434E-5	2.4578320179746596E-4
17	0.0012876902528954196	5.343113082553608E-6	0.0	2.6715565412768042E-5	2.7784188029278764E-4
18	0.0013304351575558484	5.343113082553608E-6	0.0	2.6715565412768042E-5	2.885281064578948E-4
19	0.0013518076098860629	5.343113082553608E-6	0.0	4.274490466042887E-5	3.045574457055557E-4
20	0.0013571507229686165	5.343113082553608E-6	0.0	4.8088017742982476E-5	3.312730111183237E-4
21	0.001405238740711599	5.343113082553608E-6	0.0	4.8088017742982476E-5	3.4730235036598456E-4
22	0.0014266111930418134	5.343113082553608E-6	0.0	4.8088017742982476E-5	3.7401791577875256E-4
23	0.0014693560977022424	5.343113082553608E-6	0.0	4.8088017742982476E-5	4.1676282043918143E-4
24	0.0015014147761975639	5.343113082553608E-6	0.0	5.3431130825536084E-5	4.2744904660428867E-4
25	0.0015548459070231	5.343113082553608E-6	0.0	5.877424390808969E-5	4.488214989345031E-4
26	0.0016136201509311897	5.343113082553608E-6	0.0	6.41173569906433E-5	4.7019395126471754E-4
27	0.0016723943948392793	5.343113082553608E-6	0.0	6.41173569906433E-5	4.7553706434727116E-4
28	0.001720482412582262	5.343113082553608E-6	0.0	6.41173569906433E-5	4.7553706434727116E-4
29	0.0018220015611507805	5.343113082553608E-6	0.0	6.41173569906433E-5	4.8088017742982473E-4
30	0.0019128344835541919	5.343113082553608E-6	0.0	6.946047007319691E-5	5.022526297600392E-4
31	0.002019696745205264	5.343113082553608E-6	0.0	6.946047007319691E-5	5.236250820902537E-4
32	0.0021319021199388897	5.343113082553608E-6	0.0	7.480358315575052E-5	5.396544213379144E-4
33	0.0022334212685074083	5.343113082553608E-6	0.0	8.014669623830413E-5	5.823993259983433E-4
34	0.0023402835301584803	5.343113082553608E-6	0.0	8.014669623830413E-5	5.930855521634505E-4
35	0.0025005769226350886	5.343113082553608E-6	0.0	8.014669623830413E-5	6.14458004493665E-4
36	0.002602096071203607	5.343113082553608E-6	0.0	8.014669623830413E-5	6.41173569906433E-4
37	0.0027730756898453226	5.343113082553608E-6	0.0	8.548980932085773E-5	6.518597960715403E-4
38	0.002933369082321931	5.343113082553608E-6	0.0	9.083292240341134E-5	6.518597960715403E-4
39	0.0031150349271287536	5.343113082553608E-6	0.0	9.617603548596495E-5	6.572029091540939E-4
40	0.0033768474681738804	5.343113082553608E-6	0.0	1.0686226165107217E-4	6.732322484017546E-4
41	0.003697434253127097	5.343113082553608E-6	0.0	1.2289160089873298E-4	6.892615876494155E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	152335	0.0	44.559525	12
GTATCAA	152080	0.0	44.39937	9
AAGCAGT	153405	0.0	44.385063	1
GGTATCA	152225	0.0	44.35244	8
GTGGTAT	152890	0.0	44.207176	6
ACGCAGA	153545	0.0	44.163994	15
TGGTATC	152885	0.0	44.160976	7
AGTGGTA	152715	0.0	44.142426	5
ATCAACG	153760	0.0	44.085426	11
CAACGCA	154030	0.0	44.073753	13
CGCAGAG	155020	0.0	43.754387	16
AACGCAG	155235	0.0	43.71952	14
TATCAAC	154570	0.0	43.664364	10
AGAGTAC	151990	0.0	43.518063	19
CAGTGGT	156745	0.0	43.16492	4
AGCAGTG	158100	0.0	43.127926	2
GCAGTGG	158570	0.0	42.955635	3
CAGAGTA	154850	0.0	42.923737	18
GCAGAGT	156015	0.0	42.857773	17
GTACATG	103215	0.0	42.34622	22
>>END_MODULE