FastQCFastQC Report
Thu 26 May 2016
SRR805375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30475548
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6552532.1500942329240478No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG979530.32141505708117213No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG555930.1824183768574071No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC446100.14637964836596212No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG338680.11113171779552579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT3311200.045.1767461
GTGGTAT3306300.045.0439766
AGTGGTA3302450.044.9925965
GGTATCA3308750.044.9907388
GTATCAA3307150.044.986219
TCAACGC3325550.044.96272712
TGGTATC3308500.044.9522257
ATCAACG3335550.044.73986411
ACGCAGA3347100.044.6311115
CAACGCA3351300.044.6228613
TATCAAC3345200.044.47381610
AACGCAG3373350.044.3193414
CGCAGAG3373000.044.27377716
CAGTGGT3378100.044.156854
AGAGTAC3301200.044.11909519
GCAGTGG3410450.044.0005263
AGCAGTG3405150.043.977232
GCAGAGT3366700.043.66908317
CAGAGTA3352150.043.6644418
GAGTACA2239000.043.15435420
Produced by FastQC (version 0.11.5)