##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30475548
Sequences flagged as poor quality	0
Sequence length	53
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.125942936284524	35.0	35.0	37.0	33.0	37.0
2	35.39984314638083	37.0	35.0	37.0	33.0	37.0
3	37.20873852703157	39.0	37.0	39.0	34.0	39.0
4	37.20855949825742	39.0	37.0	39.0	34.0	39.0
5	37.200714553188675	39.0	37.0	39.0	34.0	39.0
6	37.224180054120765	39.0	37.0	39.0	34.0	39.0
7	36.969421156922266	39.0	37.0	39.0	33.0	39.0
8	37.989667782183936	40.0	38.0	40.0	34.0	40.0
9	37.94538142513467	40.0	38.0	40.0	33.0	40.0
10	37.598375523879014	40.0	37.0	40.0	33.0	40.0
11	37.70774386075026	40.0	37.0	40.0	33.0	40.0
12	37.70635881592679	40.0	37.0	40.0	33.0	40.0
13	37.78234908195908	40.0	37.0	40.0	33.0	40.0
14	37.730135615608944	40.0	37.0	40.0	33.0	40.0
15	37.6421805442186	40.0	37.0	40.0	33.0	40.0
16	37.61604139161009	40.0	37.0	40.0	33.0	40.0
17	37.56787477619763	40.0	37.0	40.0	32.0	40.0
18	37.52663627246342	40.0	37.0	40.0	32.0	40.0
19	37.46342326641673	40.0	37.0	40.0	32.0	40.0
20	37.255831133865094	40.0	37.0	40.0	32.0	40.0
21	37.133494990803776	39.0	37.0	40.0	32.0	40.0
22	37.099347450618446	39.0	37.0	40.0	32.0	40.0
23	36.63791177766516	39.0	36.0	40.0	30.0	40.0
24	36.72568257017068	39.0	36.0	40.0	30.0	40.0
25	36.72296652385053	39.0	36.0	40.0	30.0	40.0
26	36.7425089452042	39.0	36.0	40.0	30.0	40.0
27	36.73449071366986	39.0	36.0	40.0	30.0	40.0
28	36.65506720338548	39.0	36.0	40.0	30.0	40.0
29	36.626524090723485	39.0	36.0	40.0	30.0	40.0
30	36.56504700095959	39.0	36.0	40.0	30.0	40.0
31	36.404220229280206	39.0	35.0	40.0	30.0	40.0
32	36.291728798445234	39.0	35.0	40.0	30.0	40.0
33	36.14224876284423	39.0	35.0	40.0	30.0	40.0
34	36.11662038037839	39.0	35.0	40.0	30.0	40.0
35	36.05500711586876	38.0	35.0	40.0	29.0	40.0
36	35.95011374364786	38.0	35.0	40.0	29.0	40.0
37	35.85363688948268	38.0	35.0	40.0	29.0	40.0
38	35.75697152353093	38.0	35.0	40.0	29.0	40.0
39	35.9116429670108	38.0	35.0	40.0	29.0	40.0
40	35.99703762504943	38.0	35.0	40.0	29.0	40.0
41	35.98895389838437	38.0	35.0	40.0	29.0	40.0
42	35.91112297636125	38.0	35.0	40.0	29.0	40.0
43	35.88308390713762	38.0	35.0	40.0	29.0	40.0
44	35.734375604993225	38.0	34.0	40.0	28.0	40.0
45	35.578543624547784	38.0	34.0	40.0	28.0	40.0
46	35.49355742511997	38.0	34.0	40.0	28.0	40.0
47	35.42659925262049	38.0	34.0	40.0	28.0	40.0
48	35.31034352524194	38.0	34.0	40.0	27.0	40.0
49	35.143843976160824	38.0	34.0	40.0	27.0	40.0
50	34.96952704509202	38.0	33.0	40.0	26.0	40.0
51	34.78356405600976	37.0	33.0	40.0	26.0	40.0
52	34.431906523879405	37.0	33.0	40.0	25.0	40.0
53	33.927701447731145	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	16.0
12	50.0
13	144.0
14	352.0
15	907.0
16	2216.0
17	5285.0
18	10790.0
19	20635.0
20	35613.0
21	56234.0
22	82386.0
23	114022.0
24	152736.0
25	200482.0
26	256067.0
27	322185.0
28	401921.0
29	497237.0
30	614901.0
31	761097.0
32	948887.0
33	1196824.0
34	1545162.0
35	2063682.0
36	2883340.0
37	4255659.0
38	6244756.0
39	7801958.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.783596606695966	37.71799608000486	24.607737980626304	18.890669332672868
2	27.32455212946458	39.9589434782272	16.051117440119533	16.665386952188683
3	35.10159029790047	24.595921950279614	19.057901764391573	21.244585987428348
4	22.35405578268847	24.520930681869938	19.613458632474796	33.5115549029668
5	20.88890739552903	34.72968558268419	21.667948349936154	22.713458671850624
6	32.16891456718022	23.089133622798187	22.44275312128924	22.299198688732357
7	21.618039485294897	22.881396587191805	32.783328457293045	22.717235470220256
8	31.569286301266843	23.92897742150527	22.91187019836362	21.589866078864276
9	32.17119180268719	23.82097280088286	22.503506745801584	21.504328650628366
10	21.24799199673128	24.20676734016399	33.50330566656258	21.041934996542146
11	21.120893379833564	34.568776253014384	23.18631645278372	21.124013914368334
12	20.97621673611907	23.609596782312167	33.9144680843803	21.49971839718846
13	21.166972944998395	23.777045781096373	23.343914931406648	31.712066342498584
14	21.33693215295095	34.23547297656469	23.19151406235583	21.236080808128534
15	21.213961435574515	34.00140335458447	23.146402486347416	21.6382327234936
16	21.532757999954587	23.443575157368787	23.097697865843134	31.92596897683349
17	31.887315036960125	23.43793785102732	23.15771975617961	21.517027355832948
18	21.36833765876827	23.428422025421824	23.11123330743716	32.09200700837275
19	21.617501348950313	33.915331071323145	23.028084679560152	21.43908290016639
20	32.150578555634176	23.551110549349268	23.090948192301582	21.207362702714978
21	21.68455182495816	33.72898167409492	23.066482676537923	21.519983824408996
22	32.047952017138456	23.331268727308856	23.27339281971238	21.347386435840303
23	21.686806091230913	23.444746588314015	33.52188121440835	21.346566106046723
24	21.639827444612315	33.57400825081144	23.092444473845063	21.69371983073118
25	21.52476798776514	23.4754794237006	23.061416319732793	31.938336268801464
26	21.64994703294589	30.273060225200872	26.642451187424093	21.434541554429146
27	21.825819178050548	23.2280220194892	33.40024599393586	21.545912808524395
28	28.636331658416776	23.278485427070912	26.59351687457761	21.491666039934703
29	28.709895552985625	23.413623275945685	26.43435320670854	21.44212796436015
30	28.623777331255862	23.305185521192268	26.48333017670429	21.587706970847577
31	26.53185760597316	24.230248460175353	26.996216770244786	22.241677163606706
32	24.896750010861165	25.112572216913048	27.331964629479344	22.65871314274644
33	24.53247436272516	24.52996415355681	27.8349580457093	23.102603438008728
34	23.781331183938022	24.557497702748446	28.04115614262293	23.6200149706906
35	23.437567061960625	24.80608388075581	28.438107167096717	23.318241890186847
36	23.7435139804541	24.686371513319465	28.4448404340424	23.125274072184034
37	24.316684969865022	24.531824661528645	28.176792752012204	22.97469761659413
38	24.167355415561353	24.75029161083502	28.14810089715204	22.934252076451585
39	24.290929895665865	24.6422246451483	28.077283466732084	22.98956199245375
40	23.728305066081173	25.295128409175778	27.81220209723546	23.164364427507586
41	24.071340735201872	25.386296581114802	27.738254288323215	22.80410839536011
42	24.023384255469335	25.360075559592893	27.79233699095419	22.824203193983582
43	23.80765064503516	25.090613628998565	28.367604743317493	22.73413098264878
44	24.212352145398665	24.779866140553075	27.956452169457297	23.051329544590963
45	24.32413028307153	24.426192434669264	27.919625924364016	23.330051357895186
46	23.812982788693414	24.61919962850217	27.69949206491709	23.868325517887325
47	23.666537513943968	24.722072265935957	28.030964365267526	23.580425854852553
48	23.61268122233602	24.531286525184058	27.986213734368288	23.869818518111636
49	23.31669638885575	25.431463939549175	27.652890770003545	23.598948901591534
50	24.10192262990644	25.172823799591725	27.545952578112786	23.179300992389045
51	23.905200982768218	24.764762228393728	27.875794062833588	23.454242726004466
52	24.3844212415803	24.670975563753604	27.590900088162485	23.353703106503616
53	24.73994233015925	24.88262065049659	26.988020034947365	23.389416984396803
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9326.0
1	5646.5
2	1967.0
3	1818.0
4	1669.0
5	1892.0
6	2115.0
7	2424.5
8	3334.5
9	3935.0
10	5317.5
11	6700.0
12	9191.5
13	11683.0
14	15211.0
15	18739.0
16	26056.5
17	33374.0
18	44519.0
19	55664.0
20	78981.5
21	102299.0
22	492022.5
23	881746.0
24	623265.0
25	362310.0
26	359836.0
27	388425.0
28	417014.0
29	451676.0
30	486338.0
31	520577.0
32	554816.0
33	620269.0
34	685722.0
35	770157.0
36	854592.0
37	944860.0
38	1035128.0
39	1129687.5
40	1224247.0
41	1321670.0
42	1518896.0
43	1618699.0
44	1737976.5
45	1857254.0
46	1947748.5
47	2038243.0
48	2139322.0
49	2240401.0
50	2268047.0
51	2295693.0
52	2290264.5
53	2284836.0
54	2237065.0
55	2189294.0
56	2134723.5
57	2080153.0
58	1865679.5
59	1454153.5
60	1257101.0
61	1082033.0
62	906965.0
63	770791.5
64	634618.0
65	517806.0
66	400994.0
67	317066.5
68	233139.0
69	182477.0
70	131815.0
71	98423.5
72	65032.0
73	47563.0
74	30094.0
75	22486.0
76	10613.5
77	6349.0
78	4582.5
79	2816.0
80	2114.5
81	1413.0
82	1003.5
83	594.0
84	422.0
85	250.0
86	171.0
87	92.0
88	71.5
89	51.0
90	41.0
91	31.0
92	20.5
93	6.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	3.0475548E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.198979993107955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.03210535538898	30.818803527466116
2	13.609090998012519	11.485795174990317
3	4.775497686732797	6.045633940187118
4	2.2834262492943025	3.8543303443883126
5	1.333605417824715	2.8138394172742762
6	0.8790478397615341	2.225695332184905
7	0.6181178481923546	1.8258759896476482
8	0.47199791290067655	1.5934264386627488
9	0.37191770294655946	1.4125092935152077
>10	2.3259428554690755	18.69825981256911
>50	0.19558160332265426	5.674058197154687
>100	0.09715865244664713	7.36188308359962
>500	0.005007928454289418	1.416432239462678
>1k	0.001250463505429975	0.9073308848047416
>5k	1.4602398253513987E-4	0.41556611319643266
>10k+	1.0546176507522844E-4	3.450560210896056
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	655253	2.1500942329240478	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	97953	0.32141505708117213	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	55593	0.1824183768574071	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	44610	0.14637964836596212	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	33868	0.11113171779552579	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.018813640365056E-4	0.0	0.0	0.0	9.84395752292953E-6
2	3.5438247082546307E-4	0.0	0.0	0.0	2.2969234220168905E-5
3	6.037627280730112E-4	0.0	0.0	0.0	5.2501106788957496E-5
4	6.136066855959407E-4	0.0	0.0	3.2813191743098435E-6	6.562638348619687E-5
5	6.136066855959407E-4	3.2813191743098435E-6	0.0	6.562638348619687E-6	7.875166018343624E-5
6	6.595451540362785E-4	3.2813191743098435E-6	0.0	6.562638348619687E-6	1.0172089440360514E-4
7	6.595451540362785E-4	3.2813191743098435E-6	0.0	6.562638348619687E-6	1.2469012862377404E-4
8	6.595451540362785E-4	3.2813191743098435E-6	0.0	6.562638348619687E-6	1.5422200119256265E-4
9	6.661077923848982E-4	3.2813191743098435E-6	0.0	6.562638348619687E-6	1.903165121099709E-4
10	6.726704307335179E-4	3.2813191743098435E-6	0.0	6.562638348619687E-6	2.0016046963290045E-4
11	6.792330690821376E-4	3.2813191743098435E-6	0.0	6.562638348619687E-6	2.1328574633013982E-4
12	6.857957074307573E-4	3.2813191743098435E-6	0.0	6.562638348619687E-6	2.2969234220168903E-4
13	6.923583457793769E-4	3.2813191743098435E-6	0.0	6.562638348619687E-6	2.493802572475481E-4
14	6.956396649536868E-4	3.2813191743098435E-6	0.0	6.562638348619687E-6	2.789121298163367E-4
15	7.022023033023065E-4	3.2813191743098435E-6	0.0	6.562638348619687E-6	2.953187256878859E-4
16	7.022023033023065E-4	6.562638348619687E-6	0.0	9.84395752292953E-6	3.182879599080548E-4
17	7.218902183481656E-4	6.562638348619687E-6	0.0	9.84395752292953E-6	3.412571941282237E-4
18	7.317341758710951E-4	6.562638348619687E-6	0.0	9.84395752292953E-6	3.707890666970123E-4
19	7.54703410091264E-4	6.562638348619687E-6	0.0	9.84395752292953E-6	4.2000885431165997E-4
20	7.645473676141935E-4	6.562638348619687E-6	0.0	9.84395752292953E-6	4.3969676935751904E-4
21	7.842352826600526E-4	6.562638348619687E-6	0.0	9.84395752292953E-6	4.5610336522906825E-4
22	8.17048474403151E-4	6.562638348619687E-6	0.0	9.84395752292953E-6	4.856352377978568E-4
23	8.400177086233199E-4	6.562638348619687E-6	0.0	9.84395752292953E-6	5.184484295409552E-4
24	8.826748578893478E-4	6.562638348619687E-6	0.0	9.84395752292953E-6	5.414176637611242E-4
25	9.384572838526152E-4	6.562638348619687E-6	0.0	9.84395752292953E-6	5.709495363299127E-4
26	9.811144331186431E-4	6.562638348619687E-6	0.0	9.84395752292953E-6	5.873561322014619E-4
27	0.0010368968590819105	6.562638348619687E-6	0.0	9.84395752292953E-6	6.037627280730112E-4
28	0.0010795540083479386	6.562638348619687E-6	0.0	9.84395752292953E-6	6.234506431188702E-4
29	0.0011583056685313747	6.562638348619687E-6	0.0	9.84395752292953E-6	6.431385581647293E-4
30	0.0012436199670634307	6.562638348619687E-6	0.0	9.84395752292953E-6	6.628264732105884E-4
31	0.0013059650313753178	6.562638348619687E-6	0.0	9.84395752292953E-6	6.661077923848982E-4
32	0.0014536243942192607	6.562638348619687E-6	0.0	9.84395752292953E-6	7.022023033023065E-4
33	0.0015750332036687249	6.562638348619687E-6	0.0	9.84395752292953E-6	7.284528566967852E-4
34	0.0016898793747695694	6.562638348619687E-6	0.0	9.84395752292953E-6	7.415781333940246E-4
35	0.001781756311650245	6.562638348619687E-6	0.0	1.6406595871549218E-5	7.579847292655738E-4
36	0.0018966024827510896	6.562638348619687E-6	0.0	1.6406595871549218E-5	8.072045168802215E-4
37	0.0020311365688977932	6.562638348619687E-6	0.0	1.6406595871549218E-5	8.367363894490101E-4
38	0.0021689519742188064	9.84395752292953E-6	0.0	1.968791504585906E-5	8.498616661462494E-4
39	0.0023756750822003267	9.84395752292953E-6	0.0	1.968791504585906E-5	8.59705623669179E-4
40	0.0025528663176130582	9.84395752292953E-6	0.0	1.968791504585906E-5	8.958001345865873E-4
41	0.0027497454680716488	9.84395752292953E-6	0.0	1.968791504585906E-5	9.089254112838266E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	331120	0.0	45.176746	1
GTGGTAT	330630	0.0	45.043976	6
AGTGGTA	330245	0.0	44.992596	5
GGTATCA	330875	0.0	44.990738	8
GTATCAA	330715	0.0	44.98621	9
TCAACGC	332555	0.0	44.962727	12
TGGTATC	330850	0.0	44.952225	7
ATCAACG	333555	0.0	44.739864	11
ACGCAGA	334710	0.0	44.63111	15
CAACGCA	335130	0.0	44.62286	13
TATCAAC	334520	0.0	44.473816	10
AACGCAG	337335	0.0	44.31934	14
CGCAGAG	337300	0.0	44.273777	16
CAGTGGT	337810	0.0	44.15685	4
AGAGTAC	330120	0.0	44.119095	19
GCAGTGG	341045	0.0	44.000526	3
AGCAGTG	340515	0.0	43.97723	2
GCAGAGT	336670	0.0	43.669083	17
CAGAGTA	335215	0.0	43.66444	18
GAGTACA	223900	0.0	43.154354	20
>>END_MODULE