FastQCFastQC Report
Thu 26 May 2016
SRR805374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17984868
Sequences flagged as poor quality0
Sequence length53
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1621720.9017135961186926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC719200.042.2915112
AAGCAGT723350.042.204811
GGTATCA719700.041.9551938
GTATCAA720950.041.9150439
ATCAACG725850.041.77778211
TGGTATC722450.041.7662167
ACGCAGA728300.041.7082315
CGCAGAG742400.040.92874516
AGAGTAC731800.040.4939919
GTGGTAT747850.040.4262286
AGTGGTA749150.040.2745135
TATCAAC755950.039.9806210
CAGAGTA748900.039.7356818
CAGTGGT765950.039.5936434
GCAGAGT759950.039.4331217
CAACGCA778500.039.18779813
AACGCAG786200.038.78307314
AGCAGTG792500.038.5192452
GCAGTGG794500.038.4311563
GTACATG457850.038.07939522
Produced by FastQC (version 0.11.5)