##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805374_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17984868
Sequences flagged as poor quality	0
Sequence length	53
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.542376902627254	35.0	35.0	37.0	32.0	37.0
2	35.06217326699312	35.0	35.0	37.0	33.0	37.0
3	37.00306596634459	39.0	37.0	39.0	33.0	39.0
4	37.083382040946866	39.0	37.0	39.0	33.0	39.0
5	37.10612021172466	39.0	37.0	39.0	33.0	39.0
6	37.08706046661004	39.0	37.0	39.0	33.0	39.0
7	36.92433878302582	39.0	37.0	39.0	33.0	39.0
8	37.88283583732725	40.0	38.0	40.0	33.0	40.0
9	37.82231040005409	40.0	37.0	40.0	33.0	40.0
10	37.63711621347457	40.0	37.0	40.0	33.0	40.0
11	37.640366501438876	40.0	37.0	40.0	33.0	40.0
12	37.56381164432233	40.0	37.0	40.0	33.0	40.0
13	37.61314133637233	40.0	37.0	40.0	33.0	40.0
14	37.52243580547825	40.0	37.0	40.0	32.0	40.0
15	37.451581073600316	40.0	37.0	40.0	32.0	40.0
16	37.43261624160934	40.0	37.0	40.0	32.0	40.0
17	37.4047138405464	40.0	37.0	40.0	32.0	40.0
18	37.35220486466734	39.0	37.0	40.0	32.0	40.0
19	37.295965363771366	39.0	37.0	40.0	32.0	40.0
20	37.028868324193425	39.0	36.0	40.0	31.0	40.0
21	37.002577388947195	39.0	36.0	40.0	31.0	40.0
22	36.86442519344596	39.0	36.0	40.0	31.0	40.0
23	36.65154184061846	39.0	36.0	40.0	30.0	40.0
24	36.611157501962204	39.0	36.0	40.0	30.0	40.0
25	36.506092899875604	39.0	36.0	40.0	30.0	40.0
26	36.49743156302287	39.0	36.0	40.0	30.0	40.0
27	36.44468199599797	39.0	36.0	40.0	30.0	40.0
28	36.3802834694144	39.0	36.0	40.0	30.0	40.0
29	36.36004273147849	39.0	35.0	40.0	30.0	40.0
30	36.29093374496827	39.0	35.0	40.0	30.0	40.0
31	36.16598931946567	39.0	35.0	40.0	30.0	40.0
32	36.04887786777195	38.0	35.0	40.0	29.0	40.0
33	35.95118234951738	38.0	35.0	40.0	29.0	40.0
34	35.90079054236039	38.0	35.0	40.0	29.0	40.0
35	35.81974735649992	38.0	35.0	40.0	28.0	40.0
36	35.74012847911922	38.0	35.0	40.0	28.0	40.0
37	35.637906377739334	38.0	34.0	40.0	28.0	40.0
38	35.590073110350325	38.0	34.0	40.0	28.0	40.0
39	35.76829004249573	38.0	35.0	40.0	28.0	40.0
40	35.85299397248843	38.0	35.0	40.0	28.0	40.0
41	35.837762390026995	38.0	35.0	40.0	28.0	40.0
42	35.74913577347357	38.0	35.0	40.0	28.0	40.0
43	35.7059744892206	38.0	34.0	40.0	28.0	40.0
44	35.54255516359642	38.0	34.0	40.0	28.0	40.0
45	35.380901711372026	38.0	34.0	40.0	27.0	40.0
46	35.27733687008434	38.0	34.0	40.0	27.0	40.0
47	35.17939364358971	38.0	34.0	40.0	27.0	40.0
48	35.0507955354468	38.0	34.0	40.0	26.0	40.0
49	34.87824141939768	37.0	33.0	40.0	26.0	40.0
50	34.72616090371083	37.0	33.0	40.0	26.0	40.0
51	34.574055589398824	37.0	33.0	40.0	26.0	40.0
52	34.25819828091037	37.0	33.0	40.0	25.0	40.0
53	33.759776607757146	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	9.0
11	33.0
12	86.0
13	206.0
14	548.0
15	1249.0
16	2634.0
17	5349.0
18	10315.0
19	17835.0
20	28282.0
21	42970.0
22	60337.0
23	80588.0
24	105207.0
25	134115.0
26	168073.0
27	209514.0
28	258951.0
29	317975.0
30	390282.0
31	478252.0
32	587749.0
33	731207.0
34	933108.0
35	1228872.0
36	1696871.0
37	2495746.0
38	3731380.0
39	4267121.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.702577967211102	32.07182838372792	22.0630087471312	24.16258490192978
2	25.735573928037724	28.198666790326175	25.791632165440415	20.274127116195682
3	25.838516023581604	24.407579749820794	24.952326589219336	24.801577637378266
4	22.638870632800863	26.749009222642055	24.36445460706189	26.247665537495184
5	24.307918189891637	29.565882829943487	23.926858957207802	22.199340022957077
6	26.874798302661993	24.478956420475257	25.405835616919738	23.240409659943015
7	23.711494574216502	25.28212606286574	28.27687142324314	22.72950793967462
8	25.9711497465536	24.650650758181822	25.803959195029957	23.57424030023462
9	26.221176602463803	24.478283632662748	25.967758006341775	23.332781758531674
10	22.923804611743606	25.30355518872866	28.892438910310602	22.880201289217137
11	22.729936077373488	28.64158914038179	25.468766298423763	23.159708483820953
12	22.843387007344173	24.843813143360297	28.856519825444366	23.45628002385116
13	23.10435639560991	25.204305085808805	24.99869334598397	26.692645172597317
14	23.046168590172584	28.569895536625566	25.152428141257417	23.231507731944433
15	23.136983824401714	28.401976594990856	24.957458681375922	23.503580899231512
16	23.371152904764163	25.05193254684994	24.84604835576219	26.730866192623708
17	26.869054585221306	24.86933459839683	25.007795442257347	23.253815374124514
18	23.218391149715416	24.78267841609958	24.876874270080826	27.12205616410418
19	23.566722869470045	28.344806311617077	24.726336606974264	23.362134211938614
20	27.01714018696162	24.816929432009175	24.922329149149164	23.243601231880046
21	23.560890188351674	28.196409337004862	24.713814969339783	23.52888550530368
22	27.1355341612738	24.72920568557968	24.812770380077296	23.322489773069226
23	23.339315028611832	24.77312594120791	28.668567375640453	23.218991654539806
24	23.47269938261432	28.13199407412943	24.736856561860783	23.65844998139547
25	23.516063615257004	24.728193723745985	24.698813469189766	27.056929191807242
26	23.417347294403275	26.705728393447203	26.441117054626144	23.43580725752338
27	23.695875888552532	24.554175209959837	28.253651903366762	23.496296998120865
28	25.769774901878623	24.65530467057084	26.16418980667526	23.410730620875285
29	25.73461756850259	24.752347362238076	26.205485633811715	23.307549435447623
30	25.637802846259422	24.552573863761467	26.198190612241355	23.61143267773775
31	25.151677510226932	24.99738113173808	26.165440858392735	23.685500499642252
32	24.56294369244189	25.338562395898595	26.398175399452473	23.700318512207037
33	24.433056723018485	24.9725269042842	26.45969378257322	24.134722590124095
34	24.27892159119544	25.154274137569427	26.50169019867146	24.065114072563667
35	24.140071531245045	25.38317768025876	26.613050482216494	23.8637003062797
36	24.108367100609247	25.10749036356564	26.698633540151643	24.085508995673475
37	24.319544630519392	25.176465014922545	26.54512671430227	23.9588636402558
38	24.276191518336415	25.263677220205345	26.55043117358437	23.909700087873873
39	24.499740559674947	24.99409503589351	26.452954783988407	24.05320962044314
40	24.30965854183639	25.207958156823835	26.41621834533342	24.06616495600635
41	24.237842613023346	25.365879805178444	26.546844825327604	23.849432756470605
42	24.438166574255646	25.10697326218908	26.461473056126962	23.993387107428312
43	24.347951844850904	25.33201244512887	26.375005921644796	23.94502978837543
44	24.37403488310284	25.16288137338567	26.548368328307998	23.914715415203492
45	24.396715060683235	24.94176771272383	26.533644839650755	24.12787238694218
46	24.183980666413564	25.331033844674312	26.30424087627443	24.180744612637692
47	24.17683021081945	25.2421758113543	26.541401360299115	24.039592617527134
48	24.353061696088066	24.927572445902857	26.53108157368739	24.188284284321686
49	24.20858468352395	25.396822484324044	26.23917506650591	24.1554177656461
50	24.313962159744516	25.29590987267741	26.4015671396643	23.988560827913776
51	24.327673686568062	25.073978858226813	26.418464678195026	24.179882777010096
52	24.54874286539106	25.208925636818684	26.175960813279254	24.066370684511
53	24.522431857715052	25.278534154379113	26.08928239006258	24.109751597843253
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	9265.0
1	5713.0
2	2161.0
3	2017.5
4	1874.0
5	2116.5
6	2359.0
7	2733.0
8	3681.0
9	4255.0
10	5343.5
11	6432.0
12	8375.5
13	10319.0
14	13140.5
15	15962.0
16	21098.0
17	26234.0
18	33955.0
19	41676.0
20	56405.5
21	71135.0
22	177578.0
23	284021.0
24	228890.5
25	184550.5
26	195341.0
27	216397.5
28	237454.0
29	258011.0
30	278568.0
31	300006.0
32	321444.0
33	362128.5
34	402813.0
35	446550.5
36	490288.0
37	540372.5
38	590457.0
39	645131.5
40	699806.0
41	753546.5
42	867162.5
43	927038.0
44	1010644.5
45	1094251.0
46	1150122.0
47	1205993.0
48	1298665.0
49	1391337.0
50	1428298.0
51	1465259.0
52	1446533.0
53	1427807.0
54	1362728.5
55	1297650.0
56	1217161.5
57	1136673.0
58	1056513.5
59	896724.0
60	817094.0
61	698553.0
62	580012.0
63	490974.0
64	401936.0
65	329314.0
66	256692.0
67	205616.5
68	154541.0
69	121584.5
70	88628.0
71	67989.0
72	47350.0
73	33787.0
74	20224.0
75	14932.5
76	7216.5
77	4792.0
78	3624.5
79	2457.0
80	2053.5
81	1650.0
82	1262.5
83	875.0
84	598.5
85	322.0
86	227.0
87	132.0
88	101.0
89	70.0
90	55.0
91	40.0
92	27.5
93	10.5
94	6.0
95	5.0
96	4.0
97	4.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.7984868E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.69188103446708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.94941341681294	35.46220975895388
2	11.741216800818986	10.964389961274525
3	4.528332145370348	6.343090374485562
4	2.188867415590092	4.088093478758159
5	1.293140033192215	3.0189570295358856
6	0.8137527120821303	2.2797386894408262
7	0.5886465078895625	1.9239508902413758
8	0.4378378145435925	1.6354776919248384
9	0.3341252635438266	1.4040843350398482
>10	1.969114333094924	16.191507155927685
>50	0.08231029077214277	2.621988891230738
>100	0.05501558370669727	5.530132195585519
>500	0.01363563588736645	4.460948748055891
>1k	0.004474983319019015	2.7685264317151055
>5k	8.194436357993694E-5	0.28280067873893266
>10k+	3.511901296283008E-5	1.024103689091089
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	162172	0.9017135961186926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.007572365835546E-4	0.0	0.0	0.0	1.6680689566362123E-5
2	9.174379261499167E-4	0.0	0.0	0.0	2.7801149277270202E-5
3	9.229981560053707E-4	0.0	0.0	0.0	4.448183884363232E-5
4	9.396788455717329E-4	0.0	0.0	0.0	7.228298812090252E-5
5	9.396788455717329E-4	0.0	0.0	0.0	8.896367768726464E-5
6	0.0010564436725362678	0.0	0.0	5.56022985545404E-6	1.0564436725362677E-4
7	0.0010564436725362678	0.0	0.0	5.56022985545404E-6	1.1120459710908081E-4
8	0.0010620039023917218	0.0	0.0	5.56022985545404E-6	1.2788528667544293E-4
9	0.0010731243621026297	0.0	0.0	1.6680689566362123E-5	1.4456597624180504E-4
10	0.0010842448218135378	0.0	0.0	1.6680689566362123E-5	1.5568643595271312E-4
11	0.001089805051668992	0.0	0.0	1.6680689566362123E-5	1.668068956636212E-4
12	0.001089805051668992	0.0	0.0	1.6680689566362123E-5	1.779273553745293E-4
13	0.001106485741235354	0.0	0.0	1.6680689566362123E-5	2.1128873450725354E-4
14	0.0011287266606571703	0.0	0.0	2.224091942181616E-5	2.2240919421816162E-4
15	0.0011342868905126243	0.0	0.0	2.224091942181616E-5	2.613308032063399E-4
16	0.0011509675800789865	0.0	0.0	2.224091942181616E-5	3.0581264204997225E-4
17	0.0011787687293562566	0.0	0.0	2.224091942181616E-5	3.336137913272424E-4
18	0.0011954494189226187	0.0	0.0	3.336137913272425E-5	3.447342510381505E-4
19	0.0012121301084889809	0.0	0.0	3.336137913272425E-5	3.502944808936046E-4
20	0.001228810798055343	0.0	0.0	3.336137913272425E-5	3.7253540031542074E-4
21	0.0012399312577662512	0.0	0.0	3.336137913272425E-5	4.003365495926909E-4
22	0.0013010937861762455	0.0	0.0	3.336137913272425E-5	4.2257746901450707E-4
23	0.0013122142458871537	0.0	0.0	3.336137913272425E-5	4.4481838843632324E-4
24	0.0013455756250198778	0.0	0.0	3.336137913272425E-5	4.5593884814723135E-4
25	0.0014122983832853262	0.0	0.0	3.336137913272425E-5	4.837399974245015E-4
26	0.0014567802221289586	0.0	0.0	3.336137913272425E-5	4.948604571354096E-4
27	0.001512382520683499	0.0	0.0	3.336137913272425E-5	5.171013765572258E-4
28	0.001640267807358942	0.0	0.0	3.336137913272425E-5	5.33782066123588E-4
29	0.0017125507954798445	0.0	0.0	3.336137913272425E-5	5.44902525834496E-4
30	0.0018237553925889254	0.0	0.0	3.336137913272425E-5	5.838241348226742E-4
31	0.001929399759842552	5.56022985545404E-6	0.0	3.336137913272425E-5	6.116252840999445E-4
32	0.0020128032076743626	5.56022985545404E-6	0.0	3.336137913272425E-5	6.394264333772147E-4
33	0.0021462487242052595	5.56022985545404E-6	0.0	3.336137913272425E-5	6.505468930881228E-4
34	0.0022574533213143406	5.56022985545404E-6	0.0	3.336137913272425E-5	6.561071229435768E-4
35	0.002363097688567967	5.56022985545404E-6	0.0	3.336137913272425E-5	6.672275826544848E-4
36	0.002457621596110686	5.56022985545404E-6	0.0	3.336137913272425E-5	6.89468502076301E-4
37	0.0025577057335088586	5.56022985545404E-6	0.0	3.336137913272425E-5	6.950287319317551E-4
38	0.00275231377844975	5.56022985545404E-6	0.0	3.336137913272425E-5	7.117094214981172E-4
39	0.002874638835269739	5.56022985545404E-6	0.0	3.336137913272425E-5	7.283901110644793E-4
40	0.0030803673399215385	5.56022985545404E-6	0.0	3.336137913272425E-5	7.617514901972035E-4
41	0.003269415155006976	5.56022985545404E-6	0.0	3.336137913272425E-5	7.951128693299278E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	71920	0.0	42.29151	12
AAGCAGT	72335	0.0	42.20481	1
GGTATCA	71970	0.0	41.955193	8
GTATCAA	72095	0.0	41.915043	9
ATCAACG	72585	0.0	41.777782	11
TGGTATC	72245	0.0	41.766216	7
ACGCAGA	72830	0.0	41.70823	15
CGCAGAG	74240	0.0	40.928745	16
AGAGTAC	73180	0.0	40.49399	19
GTGGTAT	74785	0.0	40.426228	6
AGTGGTA	74915	0.0	40.274513	5
TATCAAC	75595	0.0	39.98062	10
CAGAGTA	74890	0.0	39.73568	18
CAGTGGT	76595	0.0	39.593643	4
GCAGAGT	75995	0.0	39.43312	17
CAACGCA	77850	0.0	39.187798	13
AACGCAG	78620	0.0	38.783073	14
AGCAGTG	79250	0.0	38.519245	2
GCAGTGG	79450	0.0	38.431156	3
GTACATG	45785	0.0	38.079395	22
>>END_MODULE