FastQCFastQC Report
Thu 26 May 2016
SRR805373_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805373_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18243047
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6944353.8065735400451475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC406830.22300550998964153No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG329240.18047423766435508No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA298910.16384872548977153No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG286050.15679946447542453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG249950.13701110346314407No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA236020.12937531762100923No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG223780.1226659121143524No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC217320.11912483698583905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2530100.045.4981041
GGTATCA2525100.045.126598
GTATCAA2527750.045.085799
TGGTATC2524850.045.052877
TCAACGC2553250.044.96316512
GTGGTAT2540200.044.8490876
ATCAACG2557350.044.8313511
ACGCAGA2559500.044.80011715
AGTGGTA2540800.044.769135
CGCAGAG2568750.044.6150116
AGAGTAC2513100.044.43689319
CAGTGGT2570800.044.3892974
TATCAAC2571850.044.3364510
AGCAGTG2602300.044.261962
GCAGTGG2601300.044.2446483
CAACGCA2597600.044.21448513
CAGAGTA2537000.044.19241318
GCAGAGT2550350.044.14721317
GAGTACT1102200.044.057720
AACGCAG2605800.044.04649414