##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805373_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18243047
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.58219183451098	37.0	35.0	37.0	33.0	37.0
2	35.64599258007722	37.0	35.0	37.0	33.0	37.0
3	37.32128081454814	39.0	37.0	39.0	34.0	39.0
4	37.26327948395901	39.0	37.0	39.0	34.0	39.0
5	37.21709476492606	39.0	37.0	39.0	34.0	39.0
6	37.214521839471224	39.0	37.0	39.0	34.0	39.0
7	36.89610419794457	39.0	37.0	39.0	33.0	39.0
8	37.91966764104703	40.0	38.0	40.0	33.0	40.0
9	37.87200471500183	40.0	38.0	40.0	33.0	40.0
10	37.43494559872592	40.0	37.0	40.0	32.0	40.0
11	37.600309970149176	40.0	37.0	40.0	33.0	40.0
12	37.63393702817298	40.0	37.0	40.0	33.0	40.0
13	37.72474329534973	40.0	37.0	40.0	33.0	40.0
14	37.693697385091426	40.0	37.0	40.0	33.0	40.0
15	37.615177497487124	40.0	37.0	40.0	33.0	40.0
16	37.58336592565924	40.0	37.0	40.0	32.0	40.0
17	37.514444763531	40.0	37.0	40.0	32.0	40.0
18	37.46613309717395	40.0	37.0	40.0	32.0	40.0
19	37.388847213954996	40.0	37.0	40.0	32.0	40.0
20	37.182719641077504	39.0	37.0	40.0	32.0	40.0
21	37.03278372302609	39.0	36.0	40.0	31.0	40.0
22	37.02235476343398	39.0	36.0	40.0	31.0	40.0
23	36.44432265070632	39.0	36.0	40.0	30.0	40.0
24	36.61056204043108	39.0	36.0	40.0	30.0	40.0
25	36.624080670295925	39.0	36.0	40.0	30.0	40.0
26	36.610397100879034	39.0	36.0	40.0	30.0	40.0
27	36.630340205778126	39.0	36.0	40.0	30.0	40.0
28	36.53395976012121	39.0	36.0	40.0	30.0	40.0
29	36.49672995963887	39.0	36.0	40.0	30.0	40.0
30	36.439404064463574	39.0	36.0	40.0	30.0	40.0
31	36.26218969890282	39.0	35.0	40.0	30.0	40.0
32	36.126316179528565	39.0	35.0	40.0	30.0	40.0
33	35.976396432021474	38.0	35.0	40.0	29.0	40.0
34	35.93362495859381	38.0	35.0	40.0	29.0	40.0
35	35.866891040734586	38.0	35.0	40.0	28.0	40.0
36	35.765265637916734	38.0	35.0	40.0	28.0	40.0
37	35.60601647301572	38.0	35.0	40.0	28.0	40.0
38	35.44203180532287	38.0	34.0	40.0	28.0	40.0
39	35.63739894985744	38.0	34.0	40.0	28.0	40.0
40	35.735303647466345	38.0	34.0	40.0	29.0	40.0
41	35.69404244806254	38.0	34.0	40.0	28.0	40.0
42	35.626943733686595	38.0	34.0	40.0	28.0	40.0
43	35.60040891195424	38.0	34.0	40.0	28.0	40.0
44	35.451545676552826	38.0	34.0	40.0	27.0	40.0
45	35.28901246595484	38.0	34.0	40.0	27.0	40.0
46	35.20462289002489	38.0	34.0	40.0	27.0	40.0
47	35.103785184569226	38.0	34.0	40.0	27.0	40.0
48	34.96410824354068	37.0	33.0	40.0	26.0	40.0
49	34.76617184618337	37.0	33.0	40.0	26.0	40.0
50	34.57401244430275	37.0	33.0	40.0	26.0	40.0
51	34.34304433902955	37.0	33.0	40.0	25.0	40.0
52	33.90065108092963	36.0	32.0	40.0	23.0	40.0
53	33.37732803078345	36.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	11.0
12	31.0
13	98.0
14	206.0
15	578.0
16	1432.0
17	3281.0
18	7333.0
19	14181.0
20	24424.0
21	38541.0
22	56255.0
23	77467.0
24	103114.0
25	133524.0
26	169624.0
27	209723.0
28	259569.0
29	317861.0
30	387859.0
31	473135.0
32	586410.0
33	736071.0
34	952818.0
35	1275361.0
36	1788569.0
37	2636573.0
38	3638035.0
39	4350956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.510664857685232	41.746984481265656	24.907160519840794	21.83519014120832
2	25.984721740836385	45.427614148009376	13.599153694007365	14.988510417146871
3	37.05556423770656	24.768389841894283	18.576502050342796	19.59954387005636
4	20.333620803586154	23.11304685012323	19.818832895623192	36.73449945066742
5	19.31337456950037	37.17383395438273	22.629273498007212	20.883517978109687
6	33.8887577278072	22.75842955401036	21.357797302172166	21.995015416010276
7	21.365296049503137	22.366313039702195	35.355349355839515	20.913041554955157
8	33.40853093236015	23.62234225455868	22.580443935708765	20.388682877372403
9	33.91524453124525	22.943469914866743	22.62294780033182	20.51833775355619
10	20.42306309905357	23.747968198514208	35.65922403203807	20.169744670394152
11	20.101132228623868	37.00667986000365	22.65566163371722	20.236526277655262
12	20.043296495371635	23.223006551482325	36.19202428190861	20.541672671237432
13	20.164931877882022	23.44194475846058	22.778163099618173	33.614960264039226
14	20.418157120353854	36.88301082598757	22.462716891536815	20.236115162121767
15	20.21615687335564	36.73812823044308	22.435813491024827	20.609901405176448
16	20.49629647942035	23.188862036040362	22.53426743898648	33.78057404555281
17	33.99891476462238	22.99368082535774	22.48595862303046	20.52144578698942
18	20.47234762920909	22.977104647047174	22.516041317001488	34.034506406742246
19	20.61007681447074	36.496112738184586	22.37254006964955	20.52127037769513
20	34.208567242084065	23.23959917441423	22.34796632382737	20.20386725967433
21	20.819493585693223	36.38615851836593	22.38780615979337	20.406541736147478
22	33.885901845234514	23.00125083271451	22.666635677691342	20.44621164435963
23	20.75124292559242	23.021834017091553	35.93246237868049	20.294460678635538
24	20.60577380522015	36.17299785501841	22.59266228936427	20.628566050397172
25	20.399985813773323	23.16110899675915	22.507084479911715	33.93182070955581
26	20.60589439910997	30.33850650058622	28.488914160008466	20.566684940295335
27	20.826340029711048	22.878009358853266	35.78157749634697	20.514073115088724
28	28.1979375484808	22.877812023397187	28.397937033215996	20.526313394906015
29	28.37204223614619	22.95950890221354	28.233216742795214	20.435232118845057
30	28.20595156061375	22.89960114667248	28.307091463394247	20.58735582931952
31	25.277350872362497	23.8713796001293	29.13653623761425	21.714733289893953
32	23.83855065439452	25.11933450590792	29.477548350338623	21.56456648935893
33	23.507761614603087	24.63854859333531	29.826892404541848	22.02679738751975
34	22.695600137411258	24.707687262988472	30.091272581822544	22.50544001777773
35	22.467513239427603	25.051264736641855	30.145622055350728	22.33559996857981
36	22.5857391037802	24.82117159485474	30.43908728624116	22.1540020151239
37	22.84415536505497	24.51243479228004	30.49891281867552	22.14449702398947
38	22.98454309743323	24.627119581504118	30.18711183499116	22.20122548607149
39	23.33121215989851	24.521232664696857	30.048554937122073	22.099000238282564
40	22.570160565830914	24.948063774653434	30.094643729197212	22.38713193031844
41	22.871935812038416	25.145048412142994	29.972339598752335	22.010676177066255
42	22.97983445418959	25.08191202927888	29.917727011282707	22.02052650524882
43	22.761608847469393	25.00288466066003	30.443488963219796	21.792017528650778
44	22.90210072911614	24.78872087541078	30.047113291984612	22.262065103488467
45	22.923325253725434	24.549369411809334	30.13413822811507	22.393167106350162
46	22.518984904221316	24.838021850187637	29.934730749748113	22.708262495842938
47	22.670127418955836	24.85265208163965	29.776319712381383	22.70090078702313
48	22.91061356142973	24.491308935398784	29.989146001761657	22.608931501409824
49	22.38727445037005	25.351598337711895	29.725653834033317	22.535473377884735
50	22.913913448778594	25.174237615021216	29.700285264846382	22.211563671353804
51	22.887596573094395	24.961625105718362	29.64177530211921	22.509003019068032
52	23.19898644124526	24.88809024062702	29.34389194962881	22.569031368498912
53	23.588959673238797	25.14183074790083	28.506477015599422	22.76273256326095
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24952.0
1	13978.5
2	3005.0
3	2408.0
4	1811.0
5	1825.0
6	1839.0
7	2055.5
8	2669.5
9	3067.0
10	4202.0
11	5337.0
12	7282.0
13	9227.0
14	11657.5
15	14088.0
16	19885.0
17	25682.0
18	34331.5
19	42981.0
20	61833.0
21	80685.0
22	485783.0
23	890881.0
24	597286.5
25	291242.0
26	278792.0
27	293917.5
28	309043.0
29	326282.0
30	343521.0
31	360096.0
32	376671.0
33	413373.5
34	450076.0
35	496053.0
36	542030.0
37	595155.0
38	648280.0
39	700785.5
40	753291.0
41	796689.0
42	894283.5
43	948480.0
44	1022180.0
45	1095880.0
46	1134975.0
47	1174070.0
48	1246072.5
49	1318075.0
50	1325348.0
51	1332621.0
52	1307568.0
53	1282515.0
54	1227960.0
55	1173405.0
56	1112393.0
57	1051381.0
58	953182.0
59	769686.5
60	684390.0
61	591410.5
62	498431.0
63	428707.5
64	358984.0
65	296629.0
66	234274.0
67	184733.0
68	135192.0
69	104829.5
70	74467.0
71	58448.0
72	42429.0
73	29231.0
74	16033.0
75	11778.0
76	5589.0
77	3655.0
78	2790.0
79	1925.0
80	1680.5
81	1436.0
82	1139.0
83	842.0
84	604.0
85	366.0
86	279.0
87	192.0
88	136.0
89	80.0
90	68.5
91	57.0
92	46.0
93	22.0
94	9.0
95	6.5
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.8243047E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.856237051575434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.87287624206085	26.761408056853348
2	15.623222610570553	12.141192825317253
3	6.1369575315604115	7.153772298652878
4	2.864241140070257	4.451745308457784
5	1.5488078225499624	3.009042195016786
6	0.9311240512899721	2.170798611680787
7	0.6430572274632445	1.7490748847628486
8	0.4571354165452542	1.4210049687962463
9	0.346198556632065	1.2106775865067905
>10	2.3214594419633956	17.051581676718957
>50	0.14648327600442693	3.9137577437958515
>100	0.08929715589815415	6.971195402945702
>500	0.014569416899232293	3.891449099801187
>1k	0.004268409978639418	2.4172906053348733
>5k	1.1493352894588909E-4	0.2690751202207657
>10k+	1.867669845370697E-4	5.4169336151379355
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	694435	3.8065735400451475	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	40683	0.22300550998964153	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTG	32924	0.18047423766435508	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	29891	0.16384872548977153	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	28605	0.15679946447542453	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATG	24995	0.13701110346314407	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23602	0.12937531762100923	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	22378	0.1226659121143524	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTC	21732	0.11912483698583905	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.318618759245646E-5	0.0	0.0	0.0	1.6444621339845257E-5
2	0.0030806257309976782	0.0	0.0	0.0	2.1926161786460344E-5
3	0.0030915888118909084	0.0	0.0	1.0963080893230172E-5	2.7407702233075428E-5
4	0.0033601842937750477	0.0	0.0	1.6444621339845257E-5	2.7407702233075428E-5
5	0.0033601842937750477	0.0	0.0	1.6444621339845257E-5	3.2889242679690514E-5
6	0.0033601842937750477	0.0	0.0	1.6444621339845257E-5	3.2889242679690514E-5
7	0.0033656658342216626	0.0	0.0	2.7407702233075428E-5	3.2889242679690514E-5
8	0.003371147374668278	0.0	0.0	3.2889242679690514E-5	3.2889242679690514E-5
9	0.003371147374668278	0.0	0.0	4.385232357292069E-5	4.385232357292069E-5
10	0.0033766289151148927	0.0	0.0	4.933386401953577E-5	4.385232357292069E-5
11	0.0033766289151148927	0.0	0.0	4.933386401953577E-5	4.933386401953577E-5
12	0.0034149996982411985	0.0	0.0	5.4815404466150855E-5	5.4815404466150855E-5
13	0.0034149996982411985	0.0	0.0	5.4815404466150855E-5	7.126002580599611E-5
14	0.003436925860027659	0.0	0.0	6.0296944912765945E-5	7.67415662526112E-5
15	0.0034424074004742737	0.0	0.0	6.0296944912765945E-5	8.222310669922628E-5
16	0.0034424074004742737	0.0	0.0	6.0296944912765945E-5	9.318618759245646E-5
17	0.003458852021814119	0.0	0.0	6.577848535938103E-5	9.318618759245646E-5
18	0.0034698151027073494	0.0	0.0	7.126002580599611E-5	9.866772803907154E-5
19	0.0034752966431539642	0.0	0.0	7.126002580599611E-5	9.866772803907154E-5
20	0.0034917412644938097	0.0	0.0	7.126002580599611E-5	1.0963080893230171E-4
21	0.0035081858858336547	0.0	0.0	7.126002580599611E-5	1.151123493789168E-4
22	0.0035246305071735	0.0	0.0	7.126002580599611E-5	1.3155697071876206E-4
23	0.0035355935880667304	0.0	0.0	7.126002580599611E-5	1.3155697071876206E-4
24	0.0035630012902998056	0.0	0.0	7.126002580599611E-5	1.3703851116537714E-4
25	0.003568482830746421	0.0	0.0	7.126002580599611E-5	1.4252005161199222E-4
26	0.003595890532979496	0.0	0.0	7.126002580599611E-5	1.4252005161199222E-4
27	0.003634261316105802	0.0	0.0	7.126002580599611E-5	1.480015920586073E-4
28	0.003661669018338877	0.0	0.0	7.67415662526112E-5	1.6444621339845256E-4
29	0.003705521341911798	0.0	0.0	8.222310669922628E-5	1.6992775384506767E-4
30	0.003771299827271179	0.0	0.0	8.770464714584138E-5	1.6992775384506767E-4
31	0.0038206336912907147	0.0	0.0	8.770464714584138E-5	1.8089083473829784E-4
32	0.0038699675553102507	0.0	0.0	8.770464714584138E-5	1.973354560781431E-4
33	0.003946709121562862	0.0	0.0	8.770464714584138E-5	2.0281699652475817E-4
34	0.004017969147368858	0.0	0.0	8.770464714584138E-5	2.0281699652475817E-4
35	0.004067303011388393	0.0	0.0	1.0414926848568663E-4	2.0829853697137325E-4
36	0.004144044577641005	0.0	0.0	1.0414926848568663E-4	2.302246987578336E-4
37	0.004215304603447001	0.0	0.0	1.0963080893230171E-4	2.4666932009767887E-4
38	0.004341380033719148	0.0	0.0	1.0963080893230171E-4	2.5763240099090903E-4
39	0.00446197392354468	0.0	0.0	1.0963080893230171E-4	2.631139414375241E-4
40	0.0045661231920303665	0.0	0.0	1.0963080893230171E-4	2.631139414375241E-4
41	0.0046647909200694376	0.0	0.0	1.2059388982553189E-4	2.9052164367059953E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	253010	0.0	45.498104	1
GGTATCA	252510	0.0	45.12659	8
GTATCAA	252775	0.0	45.08579	9
TGGTATC	252485	0.0	45.05287	7
TCAACGC	255325	0.0	44.963165	12
GTGGTAT	254020	0.0	44.849087	6
ATCAACG	255735	0.0	44.83135	11
ACGCAGA	255950	0.0	44.800117	15
AGTGGTA	254080	0.0	44.76913	5
CGCAGAG	256875	0.0	44.61501	16
AGAGTAC	251310	0.0	44.436893	19
CAGTGGT	257080	0.0	44.389297	4
TATCAAC	257185	0.0	44.33645	10
AGCAGTG	260230	0.0	44.26196	2
GCAGTGG	260130	0.0	44.244648	3
CAACGCA	259760	0.0	44.214485	13
CAGAGTA	253700	0.0	44.192413	18
GCAGAGT	255035	0.0	44.147213	17
GAGTACT	110220	0.0	44.0577	20
AACGCAG	260580	0.0	44.046494	14
>>END_MODULE