FastQCFastQC Report
Thu 26 May 2016
SRR805372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27248453
Sequences flagged as poor quality0
Sequence length53
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2003210.7351646715503446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1056400.042.4330312
AAGCAGT1068900.042.1434671
GGTATCA1058000.042.135638
GTATCAA1063000.041.9639749
TGGTATC1067200.041.8318527
ACGCAGA1075300.041.7658815
ATCAACG1076600.041.645611
GTGGTAT1076550.041.5711336
AGTGGTA1079100.041.4140975
CAACGCA1083550.041.38932413
CGCAGAG1092100.041.08465616
AACGCAG1102700.040.6961114
TATCAAC1099350.040.67476310
AGAGTAC1090650.040.1136519
CAGTGGT1119100.040.0661284
CAGAGTA1121550.039.18657718
GCAGTGG1149900.039.1421433
GCAGAGT1130950.039.04580717
AGCAGTG1156750.038.9550482
GAGTACA765000.038.19287520
Produced by FastQC (version 0.11.5)